Donkey anti-Mouse IgG Secondary Antibody [HRP]

Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB41642) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cytokeratin 10 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Cytokeratin 10 Monoclonal Antibody (Catalog # MAB11806) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021) Tissue was stained with a 30 min incubation of Donkey anti Mouse HRP conjugated secondary antibodies (Catalog # HAF018) at 1:20 dilution + DAB chromogen (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Donkey
• Conjugate: HRP

Donkey anti-Mouse IgG Secondary Antibody [HRP] Summary

• Immunogen: Mouse IgG
• Specificity: Detects mouse IgG₁, IgG_2A, IgG_2B, and IgG₃ heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Donkey
• Gene: IGHG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 dilution
  • Western Blot

Packaging, Storage & Formulations

• Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Mouse IgG Secondary Antibody [HRP]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (HAF018)(33)

Publications using HAF018

• Gai, D;Caviness, PC;Lazarenko, OP;Chen, JF;Randolph, CE;Zhang, Z;Cheng, Y;Sun, F;Xu, H;Blackburn, ML;Tricot, G;Shaughnessy, JD;Chen, JR;Zhan, F; Cystatin M/E Ameliorates Multiple Myeloma-Induced Hyper Osteolytic Bone Resorption Cancers 2025-02-27 [PMID: 40075680] (Western Blot, Mouse)
• Khorramdelazad, H;Bagherzadeh, K;Rahimi, A;Darehkordi, A;Najafi, A;Karimi, M;Khoshmirsafa, M;Hassanshahi, G;Safari, E;Falak, R; A1, an innovative fluorinated CXCR4 inhibitor, redefines the therapeutic landscape in colorectal cancer Cancer cell international 2025-01-05 [PMID: 39757159] (Western Blot, Mouse)
• Song, B;Korolkova, O; Bafilomycin A1 Inhibits HIV-1 Infection by Disrupting Lysosomal Cholesterol Transport Viruses 2024-08-29 [PMID: 39339852] (Western Blot, Human)
• Nakanishi, R;Hashimoto, N;Takuwa, M;Xing, J;Uemura, M;Un Nisa, B;Tanaka, M;Hirabayashi, T;Tanaka, M;Fujino, H; High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats Acta histochemica et cytochemica 2023-12-28 [PMID: 38318105]
• Nakanishi, R;Tanaka, M;Nisa, BU;Shimizu, S;Hirabayashi, T;Tanaka, M;Maeshige, N;Roy, RR;Fujino, H; Alternating current electromagnetic field exposure lessens intramyocellular lipid accumulation due to high-fat feeding via enhanced lipid metabolism in mice PloS one 2023-11-27 [PMID: 38011220] (Mouse)
• Manish Mishra, Manisha Yadav, Sandeep Kumar, Raj Kumar, Pradip Sen TIM-3 increases the abundance of type-2 dendritic cells during Leishmania donovani infection by enhancing IL-10 production via STAT3 Cell Death & Disease 5/18/2023 [PMID: 37202419]
• Kanokwan Sriwattanapong, Thanakorn Theerapanon, Lawan Boonprakong, Anucharte Srijunbarl, Thantrira Porntaveetus, Vorasuk Shotelersuk Novel ITGB6 variants cause hypoplastic-hypomineralized amelogenesis imperfecta and taurodontism: characterization of tooth phenotype and review of literature BDJ Open 4/11/2023 [PMID: 37041139]
• Rahul Mallick, Erika Gurzeler, Pyry I. Toivanen, Tiina Nieminen, Seppo Ylä-Herttuala Novel Designed Proteolytically Resistant VEGF-B186R127S Promotes Angiogenesis in Mouse Heart by Recruiting Endothelial Progenitor Cells Frontiers in Bioengineering and Biotechnology 8/4/2022 [PMID: 35992336]
• MN Akhtar, S Kumar, P Sen Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 Receptor MBio, 2022-08-04;13(4):e0330921. 2022-08-04 [PMID: 35924848] (Western Blot, Mouse)
• Mai M. Abdelmoaty, Jatin Machhi, Pravin Yeapuri, Farah Shahjin, Vikas Kumar, Katherine E. Olson et al. Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease Clinical and Translational Medicine 7/8/2022 [PMID: 35802825]
• Barrett P, Quick TJ, Mudera V, Player DJ. Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro Frontiers in Cell and Developmental Biology 1/11/2022 [PMID: 35087826]
• Barrett P, Quick TJ, Mudera V, Player DJ. Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro Frontiers in Cell and Developmental Biology 2022-01-11 [PMID: 35087826]
• Henna Korpela, Olli-Pekka Hätinen, Tiina Nieminen, Rahul Mallick, Pyry Toivanen, Jonna Airaksinen et al. Adenoviral VEGF-B186R127S gene transfer induces angiogenesis and improves perfusion in ischemic heart iScience 12/17/2021 [PMID: 34917905]
• Mai M. Abdelmoaty, Pravin Yeapuri, Jatin Machhi, Katherine E. Olson, Farah Shahjin, Vikas Kumar et al. Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions Frontiers in Immunology 10/4/2021 [PMID: 34671355]
• Garofalo M, Bertinato L, Staniszewska M et al. Combination Therapy of Novel Oncolytic Adenovirus with Anti-PD1 Resulted in Enhanced Anti-Cancer Effect in Syngeneic Immunocompetent Melanoma Mouse Model Pharmaceutics 4/14/2021 [PMID: 33919827]
• A Tarar, EM Alyami, CA Peng Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System Methods and protocols, 2020-12-01;3(4):. 2020-12-01 [PMID: 33271819] (Western Blot, N/A)
• Alexander Jorge Cortez, Katarzyna Aleksandra Kujawa, Agata Małgorzata Wilk, Damian Robert Sojka, Joanna Patrycja Syrkis, Magdalena Olbryt et al. Evaluation of the Role of ITGBL1 in Ovarian Cancer Cancers (Basel) 9/19/2020 [PMID: 32961775]
• A Singhal, ES Krystofiak, WG Jerome, B Song 2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy Sci Rep, 2020-05-26;10(1):8663. 2020-05-26 [PMID: 32457374] (Western Blot, Human)
• Joanne A. Matsubara, Yuan Tian, Jing Z. Cui, Matthew R. Zeglinski, Sho Hiroyasu, Christopher T. Turner et al. Retinal Distribution and Extracellular Activity of Granzyme B: A Serine Protease That Degrades Retinal Pigment Epithelial Tight Junctions and Extracellular Matrix Proteins Frontiers in Immunology 4/7/2020 [PMID: 32318066]
• Joanne A. Matsubara, Yuan Tian, Jing Z. Cui, Matthew R. Zeglinski, Sho Hiroyasu, Christopher T. Turner et al. Retinal Distribution and Extracellular Activity of Granzyme B: A Serine Protease That Degrades Retinal Pigment Epithelial Tight Junctions and Extracellular Matrix Proteins Frontiers in Immunology 2020-04-07 [PMID: 32318066] (Immunohistochemistry, Human)
• E Tkaczynski, A Arulselvan, J Tkaczynski, S Avery, L Xiao, B Torok-Stor, K Abrams, NV Rao, G Johnson, TP Kennedy, M Poncz, MP Lambert 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia Blood Adv, 2018-04-10;2(7):754-761. 2018-04-10 [PMID: 29599195] (ELISA Capture, Mouse)
• MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity Nat Commun, 2017-09-15;8(1):552. 2017-09-15 [PMID: 28916756] (Western Blot, Human)
• R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study Cell Death Dis, 2017-08-24;8(8):e3017. 2017-08-24 [PMID: 28837142] (Western Blot, Human)
• Y Xia, M Sun, Y Xie, R Shu mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress Oxid Med Cell Longev, 2017-07-19;2017(0):6292630. 2017-07-19 [PMID: 28804534] (Western Blot, Human)
• Céline Mongaret Kossmann, Maxime Annereau, Audrey Thomas-Schoemann, Carole Nicco-Overney, Christiane Chéreau, Frédéric Batteux et al. ADAM9 expression promotes an aggressive lung adenocarcinoma phenotype Tumour Biol 7/5/2017 [PMID: 28675123]
• P Bokiniec, S Shahbazian, SJ McDougall, BA Berning, D Cheng, IJ Llewellyn-, PG Burke, S McMullan, M Mühlenhoff, H Hildebrand, F Braet, M Connor, NH Packer, AK Goodchild Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract J. Neurosci., 2017-06-02;0(0):. 2017-06-02 [PMID: 28576943] (Functional Assay, Rat)
• T Poobalasin, LL Yates, SA Walker, M Pereira, NY Gross, A Ali, M Kolatsi-Jo, MR Jarvelin, J Pekkanen, E Papakrivop, DA Long, M Griffiths, D Wagner, M Königshoff, M Hind, C Minelli, CM Lloyd, CH Dean Heterozygous Vangl2(Looptail) mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair Dis Model Mech, 2017-02-24;0(0):. 2017-02-24 [PMID: 28237967] (Western Blot, Mouse)
• Kathy Ye Morgan, Demetra Sklaviadis, Zachary L. Tochka, Kristin M. Fischer, Keith Hearon, Thomas D. Morgan et al. Multi-Material Tissue Engineering Scaffold with Hierarchical Pore Architecture Advanced Functional Materials 8/23/2016 [PMID: 27942257]
• Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target Sci Rep, 2016-11-17;6(0):37360. 2016-11-17 [PMID: 27853274] (Western Blot, Human)
• Lynam-Lennon , Niamh, Maher , Stephen, Maguire , Aoife, Phelan , James, Muldoon , Cian, Reynolds , John V, O'Sullivan , Jacintha Altered mitochondrial function and energy metabolism is associated with a radioresistant phenotype in oesophageal adenocarcinoma. PLoS ONE, 2014-06-26;9(6):e100738. 2014-06-26 [PMID: 24968221] (Western Blot, Human)
• Hamish T. J. Gilbert, Navraj S. Nagra, Anthony J. Freemont, Sarah J. Millward-Sadler, Judith A. Hoyland Integrin – Dependent Mechanotransduction in Mechanically Stimulated Human Annulus Fibrosus Cells: Evidence for an Alternative Mechanotransduction Pathway Operating with Degeneration PLoS ONE 9/5/2013 [PMID: 24039840]
• Krishnatry AS, Brazeau DA, Fung HL Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin. Nitric Oxide, 2009-10-15;22(1):11-7. 2009-10-15 [PMID: 19836459] (Western Blot, Human)
• Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W Sleeping beauty transposition from nonintegrating lentivirus. Mol. Ther., 2009-05-05;17(7):1197-204. 2009-05-05 [PMID: 19417741] (Western Blot, Human)

Review for IgG Antibody (HAF018) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Pig.

reviewed by: Verified Customer WB Pig 08/14/2019

Summary

• Application: Western Blot
• Sample Tested: Muscle tissue
• Species: Pig
• Lot: WVA091611

Bioinformatics

• Gene Symbol: IGHG1