IgG Antibody (IG266)

Images

 
Immunocytochemistry/ Immunofluorescence: IgG Antibody (IG266) [NBP2-34250] - Multiple myeloma LP-1 and H929 cells stained with NBP2-34250 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: IgG Antibody (IG266) [NBP2-34250] - Formalin-fixed, paraffin embedded human Tonsil stained with IgG Monoclonal Antibody (IG266).
Immunohistochemistry-Paraffin: IgG Antibody (IG266) [NBP2-34250] - Formalin-paraffin human tonsil stained with IgG MAb (IG266)
Protein Array: IgG Antibody (IG266) [NBP2-34250] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-IgG Antibody (IG266). Z- and S- Score: The Z-score represents the strength ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PA
Clone
IG266
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

IgG Antibody (IG266) Summary

Immunogen
Purified human Ig Gamma Chain
Localization
Cytoplasm, Cell Surface and Secreted
Marker
B-Cell Marker
Specificity
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), u (IgM), Epsilon (IgE), or Delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHG1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • Protein Array
Application Notes
ELISA (For coating, order NBP2-34648 (BSA free version of NBP2-34250)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34250 in the following applications:

Publications
Read Publication using
NBP2-34250 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for IgG Antibody (IG266)

  • IgG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NBP2-34250)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Filter By Species
Human
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Review for IgG Antibody (NBP2-34250) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34250:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry IgG NBP2-34250
Enlarge
reviewed by:
Natalie Erdmann
ICC Human 05/28/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedLP-1 (multiple myeloma) and H929 (multiple myeloma)
SpeciesHuman
Lot3535-4P 190321

Comments

Comments1. Cells immersion-fixed in 2%PFA 10 minutes, wash PBS X 3
2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3
3. Block 10% donkey serum 1 hour
4. 1/200 NBP2-34250 O/N @4oC; wash PBS X 3
5. 1/1000 donkey F(ab’)2 Alexa Fluor 647 anti-mouse IgG (H+L) 1 hour @room temp (Jackson #715-606-150); wash PBS X 3
6. Olympus/BIOVIEW Cy5 filter 600ms exposure

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Antibody (NBP2-34250) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34648AF350
Alexa Fluor 405 NBP2-34648AF405
Alexa Fluor 488 NBP2-34648AF488
Alexa Fluor 532 NBP2-34648AF532
Alexa Fluor 594 NBP2-34648AF594
Alexa Fluor 647 NBP2-34648AF647
Alexa Fluor 700 NBP2-34648AF700
Alexa Fluor 750 NBP2-34648AF750
Allophycocyanin NBP2-34648APC
Allophycocyanin/Cy7 NBP2-34648APCCY7
Biotin NBP2-34648B
DyLight 350 NBP2-34648UV
DyLight 405 NBP2-34648V
DyLight 488 NBP2-34648G
DyLight 550 NBP2-34648R
DyLight 594 NBP2-34648DL594
DyLight 650 NBP2-34648C
DyLight 680 NBP2-34648FR
DyLight 755 NBP2-34648IR
FITC NBP2-34648F
HRP NBP2-34648H
Janelia Fluor 549 NBP2-34648JF549
Janelia Fluor 646 NBP2-34648JF646
PE NBP2-34648PE
PE/Atto594 NBP2-34648PEATT594
PE/Cy5.5 NBP2-34648PECY55
PE/Cy7 NBP2-34648PECY7

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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Natalie Erdmann
05/28/2019
Application: ICC
Species: Human

Bioinformatics

Gene Symbol IGHG1
Uniprot
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