IGFBP-rP10/KAZALD1 Antibody [Unconjugated]

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IGFBP-rP10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IGFBP-rP10 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

IGFBP-rP10/KAZALD1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rP10 isoform 1
  Arg31-Tyr304
  Accession # Q96I82
• Specificity: Detects human IGFBP-rP10 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse IGFBP-rP10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KAZALD1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-rP10/KAZALD1 Antibody [Unconjugated]

• bone- and odontoblast-expressed gene 1
• Bono1
• FKSG28
• FKSG40
• FLJ24094
• IGFBP-related protein 10
• IGFBPrP10
• IGFBP-rP10
• KAZALD1
• Kazal-type serine peptidase inhibitor domain 1
• Kazal-type serine protease inhibitor domain 1
• kazal-type serine protease inhibitor domain-containing protein 1
• novel kazal-type serine protease inhibitor domain and immunoglobulin domain containing protein
• UNQ2945/PRO21184

Background

IGFBP-rP10 (IGF binding protein-related protein 10; also Kazal-type serine protease inhibitor domain-contain protein 1 and BONO1) is a 37 kDa (predicted), secreted member of the IGFBP superfamily. It is expressed by osteoblasts and odontoblasts, is associated with regions of mineralization, and stimulates the proliferation of osteoblasts in vitro. Mature human IGFBP-rP10 is 274 amino acids (aa) in length. It contains an IGF binding domain (aa 49‑124), a follistatin-like serine protease inhibitor region (aa 125‑168) and a C2-type Ig‑like domain (aa 172‑269). There is one splice variant that shows a 70 aa substitution for aa 184‑304. Mature human IGFBP‑rP10 is 87% aa identical to mouse IGFBP-rP10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KAZALD1
• Uniprot:
  • Q96I82()