Mouse IGFBP-5 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. Igfbp5 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Igfbp5. |
| Sensitivity |
5 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IGFBP5 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent B (Item E): 5x concentrated buffer. For detection antibody andHRP-Streptavidin diluent.
- Assay Diluent C (Item L): Diluent buffer. For Standard/Sample(serum/plasma samples/cell culture medium) diluent.
- Detection Antibody IGFBP-5 (Item F): Biotinylated anti-Mouse IGFBP-5(each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 200x concentrated HRP-conjugatedstreptavidin.
- IGFBP-5 Microplate (Item A): Coated with anti-Mouse IGFBP-5.
- Standards (Item C): Recombinant Mouse IGFBP-5.
- Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’- tetramethylbenzidine(TMB) in buffer solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse IGFBP-5 ELISA Kit (Colorimetric)
Background
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for IGFBP-5 ELISA Kit (KA1789). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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