Human IGFBP-4 ELISA Kit (Colorimetric) Summary
| Sensitivity |
0.25 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 15% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IGFBP4 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
IGFBP4 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP4. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): Diluent buffer, 0.09% sodium azide as preservative.
- Assay Diluent B (Item E): 5X concentrated buffer.
- Detection Antibody IGFBP-4 (Item F): Biotinylated anti-Human IGFBP-4. Eachvial is enough to assay half the microplate.
- HRP-Streptavidin Concentrate (Item G): 600x concentrated HRP-conjugatedstreptavidin.
- IGFBP-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated withanti-human IGFBP-4.
- Standard Protein (Item C): Human IGFBP-4. 1 vial is enough to run eachstandard in duplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine (TMB)in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human IGFBP-4 ELISA Kit (Colorimetric)
Background
IGFBP4 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IGFBP-4 ELISA Kit (KA1873). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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