IGF2BP1 Antibody

Western Blot: IGF2BP1 Antibody [NBP1-52044] - (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP1-52044] - 3.75ug/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IGF2BP1 Antibody Summary

• Description: Novus Biologicals Goat IGF2BP1 Antibody (NBP1-52044) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-IGF2BP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKVFAEHKISYSGQ corresponding to internal region according to NP_001153895.1.
• Epitope: C-EKVFAEHKISYSGQ
• Specificity: This antibody is expected to recognize both reported isoforms (NP_006537.3; NP_001153895.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGF2BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.3-1ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:100-1:2000
• Application Notes: Western blot: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Breast.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IGF2BP1 Antibody

• Coding region determinant-binding protein
• CRD-BP
• CRDBPIGF-II mRNA-binding protein 1
• IGF II mRNA binding protein 1
• IMP1
• IMP-1ZBP-1
• insulin-like growth factor 2 mRNA binding protein 1
• insulin-like growth factor 2 mRNA-binding protein 1
• VICKZ family member 1
• VICKZ1
• ZBP1IGF2 mRNA-binding protein 1
• Zip code-binding protein 1
• Zipcode-binding protein 1

Background

The IGF2BP1 gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF2BP1 Antibody (NBP1-52044)(1)

Publication using NBP1-52044

• Noubissi FK, Goswami S, Sanek NA et al. Wnt signaling stimulates transcriptional outcome of the Hedgehog pathway by stabilizing GLI1 mRNA. Cancer Res 2009-11-01 [PMID: 19887615]

Bioinformatics

• Gene Symbol: IGF2BP1
• Entrez:
  • 10642(Human)
  • 140486(Mouse)
  • 303477(Rat)
• Uniprot:
  • NP_006537.3(Human)