Recombinant Mouse IGF-II/IGF2 Animal-Free Protein

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SDS-Page: Recombinant Mouse IGF-II Animal-Free Protein [NBP2-76217] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Animal Free
Concentration
LYOPH

Order Details

View Available Formulations

Recombinant Mouse IGF-II/IGF2 Animal-Free Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Mouse IGF-II

Source: E. coli

Amino Acid Sequence: AYGPGETLCG GELVDTLQFV CSDRGFYFSR PSSRANRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
Specificity
IGF-II is a monomer, 7.4 kDa (67 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
FDC-P1 cell proliferation, >2.0 x 10^4 units/mg (ED50 acceptance criteria are <50 ng/mL).
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IGF2
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
7.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Mouse IGF-II/IGF2 Animal-Free Protein

  • C11orf43
  • chromosome 11 open reading frame 43
  • FLJ22066
  • FLJ44734
  • GRDF
  • IGF2
  • IGF-2
  • IGFII
  • IGF-II
  • insulin-like growth factor 2 (somatomedin A)
  • insulin-like growth factor II
  • insulin-like growth factor type 2
  • MSA
  • PEG2
  • PP9974
  • somatomedin-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IGF2