IgA Antibody (7D5F12) - BSA Free

Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against HEK293 (1) and IghA1 (AA: 207-353)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of breast cancer tissues using IghA1 mouse mAb with DAB staining.

Flow Cytometry: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green) and negative control (red).

Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against human IghA1 (AA: 207-353) recombinant protein. (Expected MW is 41.7 kDa)

Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mouse mAb against L1210 (1), THP-1 (2), HepG2 (3), and COS7 (4) cell lysate.

ELISA: IgA Antibody (7D5F12) [NBP2-61794] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 7D5F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IgA Antibody (7D5F12) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human IgA (AA: 207-353) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGHA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 37.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for IgA Antibody (7D5F12) - BSA Free

• IgA
• Immunoglobulin A

Background

IGHA1 (Immunoglobulin Heavy Constant Alpha 1) is a Protein Coding gene. Diseases associated with IGHA1 include pseudotumor cerebri. Among its related pathways are Vesicle-mediated transport and Regulation of nuclear SMAD2/3 signaling. GO annotations related to this gene include antigen binding and immunoglobulin receptor binding. An important paralog of this gene is IGHG4.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHA1