IgA Antibody (2217A) [Unconjugated]

Western blot shows human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human IgA Monoclonal Antibody (Catalog # MAB4787) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgA at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 2217A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IgA Antibody (2217A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Purified IgA from human serum
• Specificity: Detects human IgA in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IgA Antibody (2217A) [Unconjugated]

• IgA
• Immunoglobulin A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgA Antibody (MAB4787)(1)

Publication using MAB4787

• MC Edman, SR Janga, Z Meng, M Bechtold, AF Chen, C Kim, L Naman, A Sarma, N Teekappana, AY Kim, S Madrigal, S Singh, E Ortiz, S Christiana, DG Arkfeld, WJ Mack, M Heur, W Stohl, SF Hamm-Alvar Increased Cathepsin S activity associated with decreased protease inhibitory capacity contributes to altered tear proteins in Sj�gren's Syndrome patients Sci Rep, 2018-07-23;8(1):11044. 2018-07-23 [PMID: 30038391] (Western Blot, Human)