IFT74 Antibody - BSA Free

Western Blot: IFT74 Antibody [NBP3-35263] - Western blot analysis of various lysates using IFT74 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IFT74 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human IFT74 (NP_001092694.1).
  
  Sequence:
  MASNHKSSAARPVSRGGVGLTGRPPSGIRPLSGNIRVATAMPPGTARPGSRGCPIGTGGVLSSQIKVAHRPVTQQGLTGMKTGTKGPQRQILDKSYYLGLLRSKISELTTEVNKLQKGIEMYNQENSVYLSYEKRAETLAVEIKELQGQLADYNMLVDKLNTNTEMEEVMNDYNMLKAQNDRETQSLDVIFTERQAKEKQIRSVEEEIEQEKQATDDIIKNMSFENQVKYLEMKTTNEKLLQELDTLQQQLDSQNMKKESLEAEIAHSQVKQEAVLLHEKLYELESHRDQMIAEDKSIGSPMEEREKLLKQIKDDNQEIASMERQLTDTKEKINQFIEEIRQLDMDLEEHQDPTNYGWKILEKNTGSFKKQV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFT74
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:1000 - 1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for IFT74 Antibody - BSA Free

• Capillary morphogenesis gene 1 protein
• CCDC2capillary morphogenesis protein 1
• CMG1Coiled-coil domain-containing protein 2
• CMG-1MGC111562
• coiled-coil domain containing 2
• FLJ22621
• intraflagellar transport 74 homolog (Chlamydomonas)
• intraflagellar transport protein 74 homolog

Background

The IFT74 gene codes for an intraflagellar transport protein 74 homolog. Isoform 1 is 600 amino acids long at 69 kDA while isoform 2 exists at 409 amino acids long at 46 kDA. IFT74 has been linked to dementia, lateral sclerosis, neuronitis, amyotrphic lateral sclerosis, and frontotemporal dementia. It is known to interact with other genes such as: IFT27, IFT52, IFT81, IFT88, and DYNC2LI1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFT74