IFN-gamma R2 Overexpression Lysate (Native)

Images

 
Western Blot: IFN gamma Receptor beta Overexpression Lysate (Adult Normal) [NBL1-11853] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFN gamma Receptor beta.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IFN-gamma R2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200674 and based on accession number NM_005534. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens interferon gamma receptor 2 (interferon gamma transducer 1) (IFNGR2), mRNA.
Gene
IFNGR2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for IFN-gamma R2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IFN-gamma R2 Overexpression Lysate (Native)

  • AF-1
  • AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1
  • IFGR2
  • IFN-gamma R2
  • IFN-gamma receptor 2
  • IFNgammaR2
  • IFN-gamma-R2
  • IFNGR2
  • IFN-gR2
  • IFNGT1
  • interferon gamma receptor 2 (interferon gamma transducer 1)
  • interferon gamma receptor 2
  • interferon gamma receptor accessory factor-1
  • interferon gamma receptor beta chain
  • Interferon gamma transducer 1

Background

This gene (IFNGR2) encodes the non-ligand-binding beta chain of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
MAB6731
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NB100-1756
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB200-310
Species: Hu, Mu, Rt, Mk
Applications: WB, ChIP, IP, IF
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-381
Species: Hu
Applications: WB, ChIP, IP, PLA
NB200-305
Species: Hu, Mu, Mk
Applications: WB, ChIP, DB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-314
Species: Hu
Applications: WB, IP, PLA
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
AF5540
Species: Mu
Applications: WB
NBP2-16468
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NBP1-89146
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB200-322
Species: Hu, Mu, Rt
Applications: WB, IHC-Fr

Publications for IFN-gamma R2 Lysate (NBL1-11853) (0)

There are no publications for IFN-gamma R2 Lysate (NBL1-11853).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IFN-gamma R2 Lysate (NBL1-11853) (0)

There are no reviews for IFN-gamma R2 Lysate (NBL1-11853). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IFN-gamma R2 Lysate (NBL1-11853) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IFN-gamma R2 Products

Bioinformatics Tool for IFN-gamma R2 Lysate (NBL1-11853)

Discover related pathways, diseases and genes to IFN-gamma R2 Lysate (NBL1-11853). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IFN-gamma R2 Lysate (NBL1-11853)

Discover more about diseases related to IFN-gamma R2 Lysate (NBL1-11853).
 

Pathways for IFN-gamma R2 Lysate (NBL1-11853)

View related products by pathway.

PTMs for IFN-gamma R2 Lysate (NBL1-11853)

Learn more about PTMs related to IFN-gamma R2 Lysate (NBL1-11853).

Blogs on IFN-gamma R2

There are no specific blogs for IFN-gamma R2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IFN-gamma R2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol IFNGR2
Entrez