IFN-beta Antibody (1020369) [Unconjugated]

Recombinant Human IFN beta ( 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC). Inhibition of anti-viral activity elicited by Recombinant Human IFN beta (50 pg/mL) is neutralized by Human IFN beta Antibody (Catalog # MAB11127). The ND50 for this effect is typically 2.00-20.0 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 1020369
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-beta Antibody (1020369) [Unconjugated] Summary

• Immunogen: CHO-expressed recombinant Human IFN-beta protein
  Met22-Asn187
  Accession # P01574
• Specificity: Detects human INFB in direct ELISAs
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-beta Antibody (1020369) [Unconjugated]

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01574()