IFN-alpha/beta R1 Antibody (85228)

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 85228
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-alpha/beta R1 Antibody (85228) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
  Lys28-Lys436
  Accession # AAA52730
• Specificity: Detects human IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) IFN‑ gamma R1, rhIFN-gamma R2, recombinant mouse IFN-alpha / beta R2, rhIL-10 R beta , rhIL-10 R alpha , or rhIL-20 R beta .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNAR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha/beta R1 Antibody (85228)

• alpha-type antiviral protein
• AVP
• beta-type antiviral protein
• CRF2-1
• Cytokine receptor class-II member 1
• Cytokine receptor family 2 member 1
• human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC
• IFN-alpha/beta R1
• IFN-alpha/beta receptor 1
• IFN-alpha-REC
• IFNAR
• IFNAR1
• IFN-aR1
• IFNBR
• IFNbR1
• IFN-bR1
• IFN-R-1
• interferon (alpha, beta and omega) receptor 1
• interferon alpha/beta receptor 1
• interferon-alpha/beta receptor alpha chain
• interferon-beta receptor 1
• Type I interferon receptor 1

Background

Type I interferons (IFN-alpha , IFN-beta , IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-alpha/beta R1 Antibody (MAB245)(6)

Publications using MAB245

• Chandler, F;Reddy, PAN;Bhutda, S;Ross, RL;Walden, M;Walker, K;Di Donato, S;Cassel, JA;Prakesch, MA;Aman, A;Datti, A;Campbell, LJ;Foglizzo, M;Bell, L;Stein, DN;Ault, JR;Al-Awar, RS;Calabrese, AN;Sicheri, F;Del Galdo, F;Salvino, JM;Greenberg, RA;Zeqiraj, E; Molecular glues that inhibit specific Zn2+-dependent DUB activity and inflammation bioRxiv : the preprint server for biology 2024-09-07 [PMID: 39282282] (Flow Cytometry, Human)
• Lan-Juan Zhao Inhibition of STAT Pathway Impairs Anti-Hepatitis C Virus Effect of Interferon Alpha Cell. Physiol. Biochem, 2016-11-18;40(2):77-90. 2016-11-18 [PMID: 27855377] (Flow Cytometry, Neutralization, Human)
• He S, Wang W, Ren H, Zhao L, Qi Z Interferon alpha and ribavirin collaboratively regulate p38 mitogen-activated protein kinase signaling in hepatoma cells. Cytokine, 2013-02-12;61(3):801-7. 2013-02-12 [PMID: 23410505] (Flow Cytometry, Functional Assay, Human)
• Madrid , Alexis S, Ganem , Don Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44. J Virol, 2012-06-06;86(16):8693-704. 2012-06-06 [PMID: 22674989] (Flow Cytometry, Human)
• Hideyuki Konishi, Koichi Okamoto, Yusuke Ohmori, Hitoshi Yoshino, Hiroshi Ohmori, Motooki Ashihara et al. An orally available, small-molecule interferon inhibits viral replication Scientific Reports 2012-02-10 [PMID: 22355771]
• Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection. J. Hepatol., 2009-05-03;51(2):322-32. 2009-05-03 [PMID: 19501422] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IFNAR1
• Uniprot:
  • AAA52730()