IFN-alpha 4 Antibody (1020125) [Unconjugated]

IFN-alpha 4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse IFN-alpha 4 Monoclonal Antibody (Catalog # MAB10259) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: IHC
• Clone: 1020125
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IFN-alpha 4 Antibody (1020125) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived mouse IFN-alpha 4
  Leu23-Glu186
  Accession # P07351
• Specificity: Detects mouse IFN-alpha 4 in direct ELISAs. In direct ELISAS, no cross-reactivity with recombinant mouse IFNA-1, -2, -6, -7, -9, -11, -12, -13, -15 or -16 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha 4 Antibody (1020125) [Unconjugated]

• IFNA4
• IFNalpha 4
• IFN-alpha 4
• IFN-alpha-4
• IFN-alpha4a
• INFA4
• interferon alpha-4
• Interferon alpha-4B
• Interferon alpha-76
• Interferon alpha-M1
• interferon, alpha 4
• MGC142200

Background

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them (4, 5). The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle (6). mIFN-A4 is a strong activator of Mx gene and  was shown to be very effective in reducing splenomegaly (7).

1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
2. Fung, K.Y. et al. (2013) Science 339:1088.
3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
6. Yeow, W.S. et al. (1998) J. Immunol. 15:2932.
7. Gerlach, N. et al. (2009) European J. Immunol. 39:136.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P07351()