Recombinant Human IFITM3/Fragilis GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP

Recombinant Human IFITM3/Fragilis GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-133 of Human IFITM3
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MNHTVQTFFSPVNSGQPPNYEMLKEEHEVAVLGAPHNPAPPTSTVIHIRSETSVPDHVVWSLFNTLFMNPCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGILMTILLIVIPVLIFQAYG

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: IFITM3
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 40.37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human IFITM3/Fragilis GST (N-Term) Protein

• 1-8U
• Fragilis
• IFITM3
• interferon induced transmembrane protein 3 (1-8U)
• interferon-induced transmembrane protein 3
• Interferon-inducible protein 1-8U
• IP15
• mil-1

Background

IFITM3( AAH06794, 1 a.a. - 134 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for IFITM3/Fragilis Recombinant Protein (H00010410-P01)(3)

Publications using H00010410-P01

• Kazi R, Siddhartha D, Andrew B et al. Cholesterol Binds the Amphipathic Helix of IFITM3 and Regulates Antiviral Activity. J Mol Biol. 2022-07-21 [PMID: 35872070]
• J Lee, ME Robinson, N Ma, D Artadji, MA Ahmed, G Xiao, T Sadras, G Deb, J Winchester, KN Cosgun, H Geng, LN Chan, K Kume, TP Miettinen, Y Zhang, MA Nix, L Klemm, CW Chen, J Chen, V Khairnar, AP Wiita, A Thomas-Tik, M Farzan, JU Jung, DM Weinstock, SR Manalis, MS Diamond, N Vaidehi, M Müschen IFITM3 functions as a PIP3 scaffold to amplify PI3K signalling in B�cells Nature, 2020-11-04;0(0):. 2020-11-04 [PMID: 33149299]
• Yao L, Dong H, Zhu H et al. Identification of the IFITM3 gene as an inhibitor of hepatitis C viral translation in a stable STAT1 cell line. Journal of Viral Hepatitis. 2011-01-01 [PMID: 21914072]

Bioinformatics

• Gene Symbol: IFITM3