IFI35 Antibody (3H6) - Azide and BSA Free

Western Blot: IFI35 Antibody (3H6) [H00003430-M01] - IFI35 monoclonal antibody (M01), clone 3H6 Analysis of IFI35 expression in A-431.

Sandwich ELISA: IFI35 Antibody (3H6) [H00003430-M01] - Detection limit for recombinant GST tagged IFI35 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IFI35 Antibody (3H6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: IFI35 (NP_005524, 189 a.a. ~ 288 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVAQRLCQIGQFTVPLGGQQVPLRVSPYVNGEIQKAEIRSQPVPRSVLVLNIPDILDGPELHDVLEIHFQKPTRGGGEVEALTVVPQGQQGLAVFTSESG
• Specificity: IFI35 - interferon-induced protein 35
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFI35
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IFI35 Antibody (3H6) - Azide and BSA Free

• FLJ21753
• ifi-35
• IFP 35
• IFP35interferon-induced 35 kDa protein
• interferon-induced protein 35

Background

IFI35, also known as Interferon-induced 35 kDa protein, consists of 2 isoforms, a 286 amino acid isoform 1 that is 31.5 kDa and a 188 amino acid isoform that is 32 kDa, has nucleus subcellular location and can be found in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Its function is not yet known. Studies of this protein are being performed on research about diverticulitis and ovarian cancer. This protein plays a role in interferon alpha/beta signaling, cytokine signaling in immune system, expression of IFN-induced genes, interferon signaling, and immune system pathways where it interacts with CLEC4G, BATF, PLEKHO1, NMI, and MAPK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFI35 Antibody (H00003430-M01)(1)

Publication using H00003430-M01

• Schmeisser H, Mejido J, Balinsky CA et al. Identification of Interferon-{alpha} induced genes associated with antiviral activity in Daudi cells, and characterization of IFIT3 as a novel antiviral gene. J Virol. 2010-08-04 [PMID: 20686046]

Bioinformatics

• Gene Symbol: IFI35
• Entrez:
  • 3430(Human)
• OMIM:
  • 600735(Human)
• Uniprot:
  • NP_005524(Human)