Recombinant Human IDI1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human IDI1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-228 of Human IDI1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MMPEINTNHL DKQQVQLLAE MCILIDENDN KIGAETKKNC HLNENIEKGL LHRAFSVFLF NTENKLLLQQ RSDAKITFPG CFTNTCCSHP LSNPAELEES DALGVRRAAQ RRLKAELGIP LEEVPPEEIN YLTRIHYKAQ SDGIWGEHEI DYILLVRKNV TLNPDPNEIK SYCYVSKEEL KELLKKAASG EIKITPWFKI IAATFLFKWW DNLNHLNQFV DHEKIYRM

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IDI1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
28.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human IDI1 Protein

  • EC 5.3.3.2
  • IPP isomerase 1
  • IPP1
  • IPPI1
  • isopentenyl diphosphate dimethylallyl diphosphate isomerase 1
  • Isopentenyl pyrophosphate isomerase 1
  • isopentenyl-diphosphate delta isomerase 1
  • isopentenyl-diphosphate delta isomerase
  • isopentenyl-diphosphate Delta-isomerase 1

Background

Isopentenyl-diphosphate isomerase 1, also known as IDI1, is a member of the IPP isomerase type I family and is involved in cholesterol biosynthesis. IDI1 is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which is the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. Recombinant human IDI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IDI1