ID2 Antibody (A4-D4)

Western Blot: ID2 Antibody (A4-D4) [NBP2-66898] - Analysis on recombinant protein lysate using anti-ID2 Mouse mAb.

Immunocytochemistry/Immunofluorescence: ID2 Antibody (A4-D4) [NBP2-66898] - Staining of F9 cells using anti-ID2 Mouse mAb.

Immunohistochemistry-Paraffin: ID2 Antibody (A4-D4) [NBP2-66898] - Analysis of paraffin- embedded human pancreatic cancer tissue using anti-ID2 Mouse mAb.

Product Discontinued

ID2 Antibody (A4-D4) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein with Human ID2 aa 1-80 / 134. (SwissProt: Q02363 Human)
• Localization: Cytoplasm Nucleus
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ID2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:1000-1:10000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for ID2 Antibody (A4-D4)

• BHLHB26
• bHLHb26cell growth-inhibiting gene 8
• Class B basic helix-loop-helix protein 26
• DNA-binding protein inhibitor ID2
• DNA-binding protein inhibitor ID-2
• GIG8
• helix-loop-helix protein ID2
• ID2
• ID2A
• ID2H
• inhibitor of differentiation 2
• Inhibitor of DNA binding 2
• inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
• MGC26389

Background

ID2 is encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ID2 Antibody (NBP2-66898)(1)

Publication using NBP2-66898

• Sa JK, Hwang JR, Cho YJ et al. Pharmacogenomic analysis of patient-derived tumor cells in gynecologic cancers Genome Biol. 2019-11-26 [PMID: 31771620] (IHC-P, Human)

Bioinformatics

• Gene Symbol: ID2