ID1 Antibody (JM92-13)

Western Blot: ID1 Antibody (JM92-13) [NBP2-66897] - Western blot analysis of ID1 on different cells lysates using anti-ID1 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2:HepG2

Immunocytochemistry/Immunofluorescence: ID1 Antibody (JM92-13) [NBP2-66897] - Staining Id1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ID1 Antibody (JM92-13) [NBP2-66897] - Analysis of paraffin-embedded human tonsil tissue using anti-Id1 antibody. Counter stained with hematoxylin.

Flow Cytometry: ID1 Antibody (JM92-13) [NBP2-66897] - Analysis of HepG2 cells with Id1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: ID1 Antibody (JM92-13) [NBP2-66897] - Analysis of Id1 on 293T cell using anti-Id1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ID1 Antibody (JM92-13) [NBP2-66897] - Staining Id1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ID1 Antibody (JM92-13) [NBP2-66897] - Staining Id1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ID1 Antibody (JM92-13) [NBP2-66897] - Analysis of paraffin-embedded human colon tissue using anti-Id1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ID1 Antibody (JM92-13) [NBP2-66897] - Analysis of paraffin-embedded human liver tissue using anti-Id1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ID1 Antibody (JM92-13) [NBP2-66897] - Analysis of paraffin-embedded human placenta tissue using anti-Id1 antibody. Counter stained with hematoxylin.

Product Discontinued

ID1 Antibody (JM92-13) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ID1 aa 11-60 / 155. (SwissProt: P41134 Human)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ID1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ID1 Antibody (JM92-13)

• BHLHB24
• bHLHb24DNA-binding protein inhibitor ID-1
• Class B basic helix-loop-helix protein 24
• dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein)
• dJ857M17.1.2
• ID
• ID1
• inhibitor of differentiation 1
• Inhibitor of DNA binding 1
• inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Background

Id1 is encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ID1 Antibody (NBP2-66897)(1)

Publication using NBP2-66897

• Shaw A Thermogenic regulation of human abdominal and neck area derived adipocytes by mitophagy, irisin and BMP7 Thesis 2022-01-01 (WB, Human)
  
  Details:
  Dilution used for WB 1:1002

Bioinformatics

• Gene Symbol: ID1