Hydrogen Potassium ATPase Beta Antibody (2G11)

Images

 
Immunocytochemistry/ Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human stomach tissues.
Immunocytochemistry/ Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) ...read more
Immunocytochemistry/ Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human gastric carcinoma tissues.
Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human gastric carcinoma tissues.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ft, RbSpecies Glossary
Applications WB, ChIP, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC, IF
Clone
2G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Hydrogen Potassium ATPase Beta Antibody (2G11) Summary

Immunogen
Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.
Epitope
This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.
Specificity
Detects the beta-subunit of hydrogen/potassium ATPase.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATP4B
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:4000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Dot Blot 1:100 - 1:2000
  • Flow Cytometry 1:50
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:2000
  • Immunohistochemistry-Paraffin 1:2000
  • Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry 1:10 - 1:500
  • Immunofluorescence 1:2000
Application Notes
DB usage was reported in scientific literature (PMID: 26686297).
Publications
Read Publications using
NB300-583 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Hydrogen Potassium ATPase Beta Antibody (2G11)

  • ATP6B
  • ATPase, H+/K+ exchanging, beta polypeptide
  • ATPase, H+/K+ transporting, beta polypeptide
  • Gastric H(+)/K(+) ATPase subunit beta
  • gastric H+/K+ ATPase beta subunit
  • gastric hydrogen-potassium ATPase, beta
  • potassium-transporting ATPase beta chain
  • potassium-transporting ATPase subunit beta
  • Proton pump beta chain

Background

The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha-subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Hydrogen Potassium ATPase Beta Antibody (NB300-583) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP4B
Entrez