HVCN1 Antibody

Western Blot: HVCN1 Antibody [NBP1-06585] - HVCN1 expression in human peripheral blood naive (CD27-) B cells and memory (CD27+) B cells

Immunohistochemistry-Paraffin: HVCN1 Antibody [NBP1-06585] - Immunohistochemistry of paraffin-embedded tonsil sections stained with anti-HVCN1

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

HVCN1 Antibody Summary

• Description: Novus Biologicals Rabbit HVCN1 Antibody (NBP1-06585) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-HVCN1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide derived from the first cytoplasmic domain at the N-termunus of human HVCN1 protein.
• Specificity: Reacts specifically with human 31 kDa protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HVCN1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:100-1:1000

Reactivity Notes

May cross react with monkeys protein due to sequence similarity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for HVCN1 Antibody

• hydrogen voltage-gated channel 1
• VSOP

Background

HVCN1 is a voltage gated proton channel highly expressed in immune tissues. Channels like HVCN1 mediate the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HVCN1 Antibody (NBP1-06585)(2)

Publications using NBP1-06585

• Yu Y, Luo X, Li C, Ding F et Al. Microglial Hv1 proton channels promote white matter injuries after chronic hypoperfusion in mice J Neurochem 2019-11-27 [PMID: 31769505]
• HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species Melania Capasso1, Mandeep K Bhamrah1, Tom Henley2, Robert S Boyd1, Claudia Langlais1, Kelvin Cain1, David Dinsdale1, Karen Pulford3, Mahmood Khan4, Boris Musset5, Vladimir V Cherny5, Deri Morgan5, Randy D Gascoyne6, Elena Vigorito2, Thomas E DeCoursey5, Ian C M MacLennan4 & Martin J S Dyer1. [PMID: 20139987]

FAQs for HVCN1 Antibody (NBP1-06585). (Showing 1 - 1 of 1 FAQs).

1. Could this product react with cultured cells from mice?
   • NBP1-06585 has not been tested on mouse cells and is not predicted to cross-react. NBP1-76278 has been validated in mouse cells so this is probably a better choice.

Bioinformatics

• Gene Symbol: HVCN1
• Entrez:
  • 84329(Human)
  • 74096(Mouse)
• Uniprot:
  • Q96D96(Human)