HUWE1 Antibody - BSA Free

Western Blot: HUWE1 Antibody [NBP1-76795] - Daudi cell lysate with HUWE1 antibody at 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody - BSA Free [NBP1-76795] - Immunofluorescence of HUWE1 in Daudi cells with HUWE1 antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody - BSA Free [NBP1-76795] - Immunocytochemistry of HUWE1 in Daudi cells with HUWE1 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HUWE1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HUWE1 Antibody - BSA Free (NBP1-76795) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1. The immunogen is located within amino acids 4250 - 4300 of ARF-BP1. Amino Acid Squence: IDIDDLKSNTEYHKYQSNS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HUWE1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5-20 ug/mL
  • Western Blot 1 ug/ml
• Theoretical MW: 481.89 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  HUWE1 Protein (NBP1-76795PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for HUWE1 Antibody - BSA Free

• arf-bp1
• EC 6.3.2
• EC 6.3.2.6
• HECT, UBA and WWE domain containing 1
• HECTH9
• HSPC272
• KIAA0312MULE
• Mcl-1 ubiquitin ligase E3
• Mule
• UREB1UBA and WWE domain-containing protein 1
• URE-binding protein 1

Background

The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HUWE1
• Entrez:
  • 10075(Human)
• Uniprot:
  • Q7Z6Z7(Human)