Human Vitamin D BP Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Vitamin D BP Quantikine ELISA Kit Summary

• Background:
  The Quantikine^® Human Vitamin D BP immunoassay is a 3.5 hour sandwich-type solid phase ELISA designed to measure human Vitamin D BP in cell culture supernates, serum, plasma, and urine. It contains HEK293-expressed recombinant human Vitamin D BP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Vitamin D BP showed linear... curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Vitamin D BP.
  Read more about the Vitamin D BP specificity in this White Paper.
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• Specificity: Natural and recombinant human Vitamin D BP
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Vitamin D BP Quantikine ELISA Kit

• DBP
• DBP/GC
• DBP-MAF
• DBPVDBG
• EC 6.3.1.5
• Gc
• gc-globulin
• Gc-MAF
• GRD3
• group-specific component (vitamin D binding protein)
• Group-specific component
• hDBP
• VDB
• VDBG
• VDBP
• Vitamin D BP
• vitamin D-binding alpha-globulin
• vitamin D-binding protein

Background

Vitamin D binding protein (VitD BP), also known as DBP and Gc-globulin, is a 58 kDa glycoprotein that circulates at high concentration in the serum and serves as a carrier protein for vitamin D. The transport of vitamin D by VitD BP is important for the function of a wide variety of tissues. VitD BP binds both the 25(OH) and the hormonally active 1,25(OH)2 forms of vitamin D. VitD BP is primarily expressed in hepatocytes and to a lesser extent in the kidney. It delivers vitamin D into cells by Megalin-mediated endocytosis. A selectively deglycosylated form of VitD BP known as macrophage activating factor (MAF) blocks the angiogenic effects of FGF basic, VEGF, and Angiopoietin 2. VitD BP enhances the chemotaxis of monocytes and neutrophils to the activated complement component C5a or C5a des Arg (a C-terminally processed form of C5a). The chemotactic cofactor property of VitD BP is eliminated by binding to 1,25(OH)2 vitamin D, but it is not altered by binding to 25(OH) vitamin D or actin.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Vitamin D BP (DVDBP0B)(35)

Publications using DVDBP0B

• A Hidalgo-Br, B Rivera-Par, G León-Reyes, N Patiño, M Castillejo, J Salmerón, R Velázquez- Unravelling the Contribution of the rs7041 and rs4588 Polymorphisms of the GC Gene and Serum VDBP Levels for Developing Metabolic Syndrome in the Mexican Population International Journal of Molecular Sciences, 2022-09-13;23(18):. 2022-09-13 [PMID: 36142493] (Human)
• SS Lee, KH Ling, M Tusimin, R Subramania, KF Rahim, SP Loh Interplay between Maternal and Neonatal Vitamin D Deficiency and Vitamin-D-Related Gene Polymorphism with Neonatal Birth Anthropometry Nutrients, 2022-01-27;14(3):. 2022-01-27 [PMID: 35276923] (Human)
• B Rivera-Par, A Hidalgo-Br, G León-Reyes, LS León-Maldo, A Aquino-Gál, M Castillejo, E Denova-Gut, YN Flores, J Salmerón, R Velázquez- Total, Bioavailable, and Free 25-Hydroxyvitamin D Equally Associate with Adiposity Markers and Metabolic Traits in Mexican Adults Nutrients, 2021-09-23;13(10):. 2021-09-23 [PMID: 34684322] (Human)
• L Karc?o?lu, A Özayd?n, ME Mavi?, GG Gürsu, L Harbige, N Hekim Vitamin-D Binding Protein Gene Polymorphisms and Serum 25-Hydroxyvitamin-D in a Turkish Population Metabolites, 2021-10-12;11(10):. 2021-10-12 [PMID: 34677411] (Human)
• MC Cho, KS Park, JK Shin, SA Lee, IA Cho, HC Jo, SC Kim, WJ Choi Correlational analysis of bone health status and vitamin D-related biomarkers in women working in agriculture Medicine, 2021-08-27;100(34):e27071. 2021-08-27 [PMID: 34449504] (Human)
• B Rivera-Par, A Hidalgo-Br, G León-Reyes, B Antuna-Pue, YN Flores, J Salmerón, R Velázquez- Association of GC Variants with Bone Mineral Density and Serum VDBP Concentrations in Mexican Population Genes, 2021-07-29;12(8):. 2021-07-29 [PMID: 34440350] (Human)
• S Alharazy, MI Naseer, E Alissa, MD Robertson, S Lanham-New, MH Alqahtani, AG Chaudhary Association of SNPs in GC and CYP2R1 with total and directly measured free 25-hydroxyvitamin D in multi-ethnic postmenopausal women in Saudi Arabia Saudi journal of biological sciences, 2021-05-04;28(8):4626-4632. 2021-05-04 [PMID: 34354449] (Human)
• JA George, SA Norris, T Snyman, L Khambule, M Toman, NJ Crowther Longitudinal changes in vitamin D and its metabolites in pregnant South Africans The Journal of steroid biochemistry and molecular biology, 2021-07-07;212(0):105949. 2021-07-07 [PMID: 34242778] (Human)
• ND Palmer, L Lu, TC Register, L Lenchik, JJ Carr, PJ Hicks, SC Smith, J Xu, L Dimitrov, J Keaton, M Guan, MCY Ng, YI Chen, AJ Hanley, CD Engelman, JM Norris, CD Langefeld, LE Wagenknech, DW Bowden, BI Freedman, J Divers Genome-wide association study of vitamin D concentrations and bone mineral density in the African American-Diabetes Heart Study PLoS ONE, 2021-05-20;16(5):e0251423. 2021-05-20 [PMID: 34014961] (Human)
• NZ Abidin, SR Mitra Total vs. Bioavailable: Determining a Better 25(OH)D Index in Association with Bone Density and Muscle Mass in Postmenopausal Women Metabolites, 2020-12-31;11(1):. 2020-12-31 [PMID: 33396337] (Human)
• U Basile, C Napodano, K Pocino, A Barini, M Marino, SA Santini, A Barini, A Stefanile, V Basile, CA Callà, P Cattani, A Gasbarrini, GL Rapaccini, F Gulli Lack of association between Vitamin D status and free light chains profile with different chronic HCV-related liver and extrahepatic disorders Eur Rev Med Pharmacol Sci, 2019-10-01;23(19):8506-8514. 2019-10-01 [PMID: 31646582] (Human)
• C Bourdon, N Lelijveld, D Thompson, PS Dalvi, GB Gonzales, D Wang, M Alipour, E Wine, E Chimwezi, JC Wells, M Kerac, R Bandsma, MJ Nyirenda Metabolomics in plasma of Malawian children 7?years after surviving severe acute malnutrition: "ChroSAM" a cohort study EBioMedicine, 2019-06-27;0(0):. 2019-06-27 [PMID: 31255658] (Human)
• F Bacha, SK Bartz, A Tomsa, S Sharma Free Vitamin D: Relationship to Insulin Sensitivity and Vascular Health in Youth J. Pediatr., 2019-06-11;0(0):. 2019-06-11 [PMID: 31201030] (Human)
• H Makino, S Seki, I Kitajima, H Motomura, M Nogami, Y Yahara, N Ejiri, T Kimura Differential proteome analysis in adolescent idiopathic scoliosis patients with thoracolumbar/lumbar curvatures BMC Musculoskelet Disord, 2019-05-24;20(1):247. 2019-05-24 [PMID: 31122237] (Human)
• KA Sauder, AV Stamatoiu, E Leshchinsk, BM Ringham, DH Glueck, D Dabelea Cord Blood Vitamin D Levels and Early Childhood Blood Pressure: The Healthy Start Study J Am Heart Assoc, 2019-05-07;8(9):e011485. 2019-05-07 [PMID: 31020895] (Human)
• C Triantos, I Aggeletopo, M Kalafateli, PI Spantidea, G Vourli, G Diamantopo, D Tapratzi, M Michalaki, S Manolakopo, C Gogos, V Kyriazopou, A Mouzaki, K Thomopoulo Prognostic significance of vitamin D receptor (VDR) gene polymorphisms in liver cirrhosis Sci Rep, 2018-09-14;8(1):14065. 2018-09-14 [PMID: 30218108] (Human)
• B Petrov, A Aldoori, C James, K Yang, GP Algorta, A Lee, L Zhang, T Lin, RA Awadhi, JR Parquette, A Samogyi, LE Arnold, MA Fristad, B Gracious, O Ziouzenkov Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein Transl Psychiatry, 2018-03-13;8(1):61. 2018-03-13 [PMID: 29531242] (Human)
• C Li, P Chen, X Duan, J Wang, B Shu, X Li, Q Ba, J Li, Y Wang, H Wang Bioavailable 25(OH)D but Not Total 25(OH)D Is an Independent Determinant for Bone Mineral Density in Chinese Postmenopausal Women EBioMedicine, 2016-11-28;15(0):184-192. 2016-11-28 [PMID: 27919752] (Human)
• Yeo Min Yun Clinical Utility of Measurement of Vitamin D-Binding Protein and Calculation of Bioavailable Vitamin D in Assessment of Vitamin D Status Ann Lab Med, 2017-01-01;37(1):34-38. 2017-01-01 [PMID: 27834063] (Human)
• Genetically Low Vitamin D Levels, Bone Mineral Density, and Bone Metabolism Markers: a Mendelian Randomisation Study Sci Rep, 2016-09-14;6(0):33202. 2016-09-14 [PMID: 27625044] (Human)
• Pharmacokinetics and effects of demographic factors on blood 25(OH)D3 levels after a single orally administered high dose of vitamin D3 Acta Pharmacol Sin, 2016-08-29;0(0):. 2016-08-29 [PMID: 27569392] (Human)
• Chaykovska L, Heunisch F, von Einem G, Alter M, Hocher C, Tsuprykov O, Dschietzig T, Kretschmer A, Hocher B Urinary Vitamin D Binding Protein and KIM-1 Are Potent New Biomarkers of Major Adverse Renal Events in Patients Undergoing Coronary Angiography. PLoS ONE, 2016-01-11;11(1):e0145723. 2016-01-11 [PMID: 26751954] (Human)
• Chao C, Lee S, Yang W, Yen C, Chiang C, Huang J, Hung K Serum vitamin D levels are positively associated with varicella zoster immunity in chronic dialysis patients. Sci Rep, 2014-12-09;4(0):7371. 2014-12-09 [PMID: 25487609] (Human)
• Ashraf A, Alvarez J, Dudenbostel T, Calhoun D, Griffin R, Wang X, Hanks L, Gower B Associations between vascular health indices and serum total, free and bioavailable 25-hydroxyvitamin D in adolescents. PLoS ONE, 2014-12-05;9(12):e114689. 2014-12-05 [PMID: 25479358] (Human)
• Anic G, Weinstein S, Mondul A, Mannisto S, Albanes D Serum vitamin D, vitamin D binding protein, and risk of colorectal cancer. PLoS ONE, 2014-07-18;9(7):e102966. 2014-07-18 [PMID: 25036524] (Human)
• Ikeda T, Kasai M, Tatsukawa E, Kamitakahara M, Shibata Y, Yokoi T, Nemoto T, Ioku K A bone substitute with high affinity for vitamin D-binding protein--relationship with niche of osteoclasts. J Cell Mol Med, 2013-11-28;18(1):170-80. 2013-11-28 [PMID: 24286277] (Human)
• Mondul A, Weinstein S, Moy K, Mannisto S, Albanes D Vitamin D-binding protein, circulating vitamin D and risk of renal cell carcinoma. Int J Cancer, 2014-01-30;134(11):2699-706. 2014-01-30 [PMID: 24214881] (Human)
• Larcombe L, Mookherjee N, Slater J, Slivinski C, Singer M, Whaley C, Denechezhe L, Matyas S, Turner-Brannen E, Nickerson P, Orr P Vitamin D in a northern Canadian first nation population: dietary intake, serum concentrations and functional gene polymorphisms. PLoS ONE, 2012-11-21;7(11):e49872. 2012-11-21 [PMID: 23185470] (Human)
• Weinstein S, Mondul A, Kopp W, Rager H, Virtamo J, Albanes D Circulating 25-hydroxyvitamin D, vitamin D-binding protein and risk of prostate cancer. Int J Cancer, 2012-12-27;132(12):2940-7. 2012-12-27 [PMID: 23180681] (Human)
• Influence of vitamin D binding protein on the association between circulating vitamin D and risk of bladder cancer. Br. J. Cancer, 2012-09-18;107(9):1589-94. 2012-09-18 [PMID: 22990651] (Human)
• Bhan I, Powe CE, Berg AH Bioavailable vitamin D is more tightly linked to mineral metabolism than total vitamin D in incident hemodialysis patients. Kidney Int., 2012-03-07;82(1):84-9. 2012-03-07 [PMID: 22398410] (Human)
• Heijboer AC, Blankenstein MA Accuracy of 6 routine 25-hydroxyvitamin D assays: influence of vitamin D binding protein concentration. Clin. Chem., 2012-01-12;58(3):543-8. 2012-01-12 [PMID: 22247500] (Human)
• Kim MJ, Frankel AH, Donaldson M, Darch SJ, Pusey CD, Hill PD, Mayr M, Tam FW Oral cholecalciferol decreases albuminuria and urinary TGF-beta1 in patients with type 2 diabetic nephropathy on established renin-angiotensin-aldosterone system inhibition. Kidney Int., 2011-08-10;80(8):851-60. 2011-08-10 [PMID: 21832985] (Human)
• Hollis BW, Johnson D, Hulsey TC, Ebeling M, Wagner CL Vitamin D supplementation during pregnancy: double-blind, randomized clinical trial of safety and effectiveness. J. Bone Miner. Res., 2011-10-01;26(10):2341-57. 2011-10-01 [PMID: 21706518] (Human)
• Nakayama S, Yasui T, Suto M, Sato M, Kaji T, Uemura H, Maeda K, Irahara M Differences in bone metabolism between singleton pregnancy and twin pregnancy. Bone, 2011-05-27;49(3):513-9. 2011-05-27 [PMID: 21640859] (Human)