Human Total Cathepsin B DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Total Cathepsin B DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CTSB

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total Cathepsin B DuoSet ELISA, 15 Plate

• APP secretase
• APPS
• Cathepsin B
• Cathepsin B1
• CPSBamyloid precursor protein secretase
• CTSB
• cysteine protease
• EC 3.4.22
• EC 3.4.22.1

Background

Cathepsin B is the first described member of the family of lysosomal cysteine proteases. Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and SLPI. Cathepsin B is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme.

Publications for Cathepsin B (DY2176)(8)

Publications using DY2176

• Duggins-Warf, M;Ghalali, A;Sesen, J;Martinez, T;Fehnel, KP;Pineda, S;Zurakowski, D;Smith, ER; Disease specific urinary biomarkers in the central nervous system Scientific reports 2023-11-07 [PMID: 37935834] (Human)
• West, PW;Tontini, C;Atmoko, H;Kiss, O;Garner, T;Bahri, R;Warren, RB;Griffiths, CEM;Stevens, A;Bulfone-Paus, S; Human Mast Cells Upregulate Cathepsin B, a Novel Marker of Itch in Psoriasis Cells 2023-08-30 [PMID: 37681909] (Human)
• Kjølle, S;Finne, K;Birkeland, E;Ardawatia, V;Winge, I;Aziz, S;Knutsvik, G;Wik, E;Paulo, JA;Vethe, H;Kleftogiannis, D;Akslen, LA; Hypoxia induced responses are reflected in the stromal proteome of breast cancer Nature communications 2023-06-22 [PMID: 37349288] (Human)
• S Li, DM Pritchard, LG Yu Galectin-3 promotes secretion of proteases that decrease epithelium integrity in human colon cancer cells Cell Death & Disease, 2023-04-13;14(4):268. 2023-04-13 [PMID: 37055381] (Human)
• LJ Rosario-Ro, Y Gerena, LA García-Req, LJ Cartagena-, JC Cuadrado-R, G Borges-Vél, LM Meléndez Cannabinoid receptor type 2 agonist JWH-133 decreases cathepsin B secretion and neurotoxicity from HIV-infected macrophages Scientific Reports, 2022-01-07;12(1):233. 2022-01-07 [PMID: 34996989] (Human)
• Bharani Thangavelu, Angela M. Boutté Single Molecule Assay for Ultrasensitive Detection of Cathepsin B in Human Blood ACS Omega 2021-04-13 [PMID: 33869941] (Human)
• H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells Oncol Lett, 2017-04-05;13(6):4224-4230. 2017-04-05 [PMID: 28599423] (Human)
• Huang Z, Tan N, Guo W, Wang L, Li H, Zhang T, Liu X, Xu Q, Li J, Guo Z Overexpression of EMMPRIN isoform 2 is associated with head and neck cancer metastasis. PLoS ONE, 2014-04-04;9(4):e91596. 2014-04-04 [PMID: 24705283] (Human)

FAQs for Cathepsin B (DY2176). (Showing 1 - 2 of 2 FAQs).

1. What's the difference between the Cathepsin B DuoSet ELISA (DY2176) and the other Cathepsin B ELISA (KA0552)? The DuoSet ELISA is much cheaper per plate.
   • The duoset contains just the antibodies.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CTSB