Human TIMP-3 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human TIMP-3 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TIMP3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TIMP-3 DuoSet ELISA, 15 Plate

• HSMRK222
• K222
• K222TA2
• metalloproteinase inhibitor 3
• MIG-5 protein
• Protein MIG-5
• pseudoinflammatory)
• SFD
• TIMP metallopeptidase inhibitor 3
• TIMP3
• TIMP-3
• tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy
• Tissue inhibitor of metalloproteinases 3

Background

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Publications for TIMP-3 (DY973)(8)

Publications using DY973

• K Du, M Wang, N Zhang, P Yu, P Wang, Y Li, X Wang, L Zhang, C Bachert Involvement of the extracellular matrix proteins periostin and tenascin C in nasal polyp remodeling by regulating the expression of MMPs Clinical and translational allergy, 2021-09-06;11(7):e12059. 2021-09-06 [PMID: 34504680] (Human)
• S Zhou, L Li, C Chen, Y Chen, L Zhou, FH Zhou, J Dong, L Wang Injectable gelatin microspheres loaded with platelet rich plasma improve wound healing by regulating early inflammation International Journal of Medical Sciences, 2021-03-03;18(9):1910-1920. 2021-03-03 [PMID: 33850460] (Rat)
• RJ Weiss, PN Spahn, AWT Chiang, Q Liu, J Li, KM Hamill, S Rother, TM Clausen, MA Hoeksema, BM Timm, K Godula, CK Glass, Y Tor, PLSM Gordts, NE Lewis, JD Esko Genome-wide screens uncover KDM2B as a modifier of protein binding to heparan sulfate Nature Chemical Biology, 2021-04-12;0(0):. 2021-04-12 [PMID: 33846619] (Human)
• DF Boyd, EK Allen, AG Randolph, XJ Guo, Y Weng, CJ Sanders, R Bajrachary, NK Lee, CS Guy, P Vogel, W Guan, Y Li, X Liu, T Novak, MM Newhams, TP Fabrizio, N Wohlgemuth, PM Mourani, PALISI Ped, TN Wight, S Schultz-Ch, SA Cormier, K Shaw-Salib, A Pekosz, RE Rothman, KF Chen, Z Yang, RJ Webby, N Zhong, JC Crawford, PG Thomas Exuberant fibroblast activity compromises lung function via ADAMTS4 Nature, 2020-10-28;0(0):. 2020-10-28 [PMID: 33116313] (Mouse)
• G Ruiz-Gómez, S Vogel, S Möller, MT Pisabarro, U Hempel Glycosaminoglycans influence enzyme activity of MMP2 and MMP2/TIMP3 complex formation - Insights at cellular and molecular level Sci Rep, 2019-03-20;9(1):4905. 2019-03-20 [PMID: 30894640] (Human)
• VS Simons, G Lochnit, J Wilhelm, B Ishaque, M Rickert, J Steinmeyer Comparative Analysis of Peptide Composition and Bioactivity of Different Collagen Hydrolysate Batches on Human Osteoarthritic Synoviocytes Sci Rep, 2018-12-07;8(1):17733. 2018-12-07 [PMID: 30531866] (Human)
• Fischer , Marlene, Dietmann , Anelia, Beer , Ronny, Broessner , Gregor, Helbok , Raimund, Pfausler , Bettina, Schmutzhard , Erich, Lackner , Peter Differential regulation of matrix-metalloproteinases and their tissue inhibitors in patients with aneurysmal subarachnoid hemorrhage. PLoS ONE, 2013-03-28;8(3):e59952. 2013-03-28 [PMID: 23555845] (Human)
• Lamblin N, Ratajczak P, Hot D, Dubois E, Chwastyniak M, Beseme O, Drobecq H, Lemoine Y, Koussa M, Amouyel P, Pinet F Profile of macrophages in human abdominal aortic aneurysms: a transcriptomic, proteomic, and antibody protein array study. J. Proteome Res., 2010-07-02;9(7):3720-9. 2010-07-02 [PMID: 20513153] (Human)

FAQs for TIMP-3 (DY973). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TIMP3