Human Thrombospondin-1 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Thrombospondin-1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: THBS1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Thrombospondin-1 DuoSet ELISA, 15 Plate

• THBS
• THBS1
• THBS-1
• thrombospondin 1
• Thrombospondin1
• Thrombospondin-1
• thrombospondin-1p180
• TSP-1
• TSP1thrombospondin-1
• TSPTSP-1

Background

Thrombospondins (TSP) are secreted multidomain glycoproteins with many putative functions including modulating cell adhesion, proliferation, migration, and angiogenesis. At least five TSPs exist: TSP-1, -2, -3, -4, and cartilage oligomeric glycoprotein (COMP)/TSP-5. The family can be divided into two subgroups. The first, TSP-1 and -2, are homotrimeric molecules, each monomer containing an N-terminal/heparin-binding domain, a coiled-coil oligomerization domain, a von Willebrand factor-type/procollagen homology domain, three type 1 thrombospondin repeats (TSR), three EGF-like repeats, seven TSP type 3 repeats, and a C-terminal globular domain. TSP-1 and -2 are thought to mediate interactions between cells and the extracellular matrix, and as such are matricellular proteins. In contrast, TSP-3, -4, and COMP/TSP-5 are homopentamers that lack the N-terminal structure of TSP-1 and -2, but have a similar arrangement of EGF and TSP type 3 repeats, and the C-terminal domain characteristic of the TSP family.

Publications for Thrombospondin-1 (DY3074)(12)

Publications using DY3074

• Lengvenyte, A;Cognasse, F;Hamzeh-Cognasse, H;Sénèque, M;Strumila, R;Olié, E;Courtet, P; Baseline circulating biomarkers, their changes, and subsequent suicidal ideation and depression severity at 6 months: A prospective analysis in patients with mood disorders Psychoneuroendocrinology 2024-07-06 [PMID: 39003840] (Human)
• Zisman, D;Sabtan, H;Rahat, MM;Simanovich, E;Haddad, A;Gazitt, T;Feld, J;Slobodin, G;Kibari, A;Elias, M;Rahat, MA; Tofacitinib Regulates Endostatin via Effects on CD147 and Cathepsin S International journal of molecular sciences 2024-07-02 [PMID: 39000375] (Human)
• J Jiang, X Cong, S Alageel, U Dornseifer, AF Schilling, E Hadjipanay, HG Machens, P Moog In Vitro Comparison of Lymphangiogenic Potential of Hypoxia Preconditioned Serum (HPS) and Platelet-Rich Plasma (PRP) International Journal of Molecular Sciences, 2023-01-19;24(3):. 2023-01-19 [PMID: 36768283] (Human)
• S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis Journal of ovarian research, 2022-07-26;15(1):87. 2022-07-26 [PMID: 35883110] (Human)
• GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion Redox Biology, 2021-12-05;48(0):102205. 2021-12-05 [PMID: 34891098] (Human)
• M Tutter, C Schug, KA Schmohl, S Urnauer, C Kitzberger, N Schwenk, M Petrini, C Zach, S Ziegler, P Bartenstei, WA Weber, G Multhoff, E Wagner, LH Lindner, PJ Nelson, C Spitzweg Regional hyperthermia enhances mesenchymal stem cell recruitment to tumor stroma: Implications for mesenchymal stem cell-based tumor therapy Mol Ther, 2020-10-15;0(0):. 2020-10-15 [PMID: 33068779] (Human)
• MA Rahat, M Safieh, E Simanovich, E Pasand, T Gazitt, A Haddad, M Elias, D Zisman The role of EMMPRIN/CD147 in regulating angiogenesis in patients with psoriatic arthritis Arthritis Res Ther, 2020-10-14;22(1):240. 2020-10-14 [PMID: 33054815] (Human)
• JH Ko, HJ Kim, HJ Jeong, HJ Lee, JY Oh Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis Cell Rep, 2020-03-17;30(11):3806-3820.e6. 2020-03-17 [PMID: 32187551] (Human)
• S Esteban, C Clemente, A Koziol, P Gonzalo, C Rius, F Martínez, PM Linares, M Chaparro, A Urzainqui, V Andrés, M Seiki, JP Gisbert, AG Arroyo Endothelial MT1-MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis EMBO Mol Med, 2019-12-03;0(0):e10862. 2019-12-03 [PMID: 31793743] (Human)
• Dong Hyun Kim, Hoon Lim, Dahm Lee, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Jeong Su Oh, Hyun Ho Hwang, Hong Bae Jeon Thrombospondin-1 secreted by human umbilical cord blood-derived mesenchymal stem cells rescues neurons from synaptic dysfunction in Alzheimer's disease model. Scientific Reports, 2018-01-10;0(0):2045-2322. 2018-01-10 [PMID: 29321508] (Human)
• S Sabrkhany, MJE Kuijpers, SMJ van Kuijk, L Sanders, S Pineda, SWM Olde Damin, AC Dingemans, AW Griffioen, MGA Oude Egbri A combination of platelet features allows detection of early-stage cancer Eur. J. Cancer, 2017-05-17;80(0):5-13. 2017-05-17 [PMID: 28527393] (Human)
• Ramon LA, Braza-Boils A, Gilabert-Estelles J, Gilabert J, Espana F, Chirivella M, Estelles A microRNAs expression in endometriosis and their relation to angiogenic factors. Hum. Reprod., 2011-02-18;26(5):1082-90. 2011-02-18 [PMID: 21335415] (Human)

FAQs for Thrombospondin-1 (DY3074). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: THBS1