Human Pro-Cathepsin B Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Pro-Cathepsin B Quantikine ELISA Kit Summary

• Background: The Quantikine Human Pro-Cathepsin B Immunoassay is a 4.5 hour solid phase ELISA designed to measure the pro-form of cathepsin B in cell culture supernates, cell lysates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human Pro-Cathepsin B and antibodies raised against the recombinant factor. Natural human Pro-Cathepsin B showed dose-response curves that were paralle...l to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human Pro-Cathepsin B.
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• Specificity: Natural and recombinant human Pro-Cathepsin B
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CTSB

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Pro-Cathepsin B Quantikine ELISA Kit

• APP secretase
• APPS
• Cathepsin B
• Cathepsin B1
• CPSBamyloid precursor protein secretase
• CTSB
• cysteine protease
• EC 3.4.22
• EC 3.4.22.1

Background

Cathepsin B is the first described member of the family of lysosomal cysteine proteases. Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and SLPI. Cathepsin B is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Cathepsin B (DCATB0)(9)

Publications using DCATB0

• Rodriguez-Franco EJ, Cantres-Rosario YM, Plaud-Valentin M, Romeu R, Rodriguez Y, Skolasky R, Melendez V, Cadilla CL, Melendez LM Dysregulation of macrophage-secreted cathepsin B contributes to HIV-1-linked neuronal apoptosis. PLoS ONE, 2012-05-31;7(5):e36571. 2012-05-31 [PMID: 22693552] (Human)
• Noda S, Asano Y, Akamata K, Aozasa N, Taniguchi T, Takahashi T, Ichimura Y, Toyama T, Sumida H, Yanaba K, Tada Y, Sugaya M, Kadono T, Sato S A possible contribution of altered cathepsin B expression to the development of skin sclerosis and vasculopathy in systemic sclerosis. PLoS ONE, 2012-02-23;7(2):e32272. 2012-02-23 [PMID: 22384200] (Human)
• Chang KP, Wu CC, Chen HC Identification of candidate nasopharyngeal carcinoma serum biomarkers by cancer cell secretome and tissue transcriptome analysis: potential usage of cystatin A for predicting nodal stage and poor prognosis. Proteomics, 2010-07-01;10(14):2644-60. 2010-07-01 [PMID: 20461718] (Human)
• Elsaid KA, Fleming BC, Oksendahl HL, Machan JT, Fadale PD, Hulstyn MJ, Shalvoy R, Jay GD Decreased lubricin concentrations and markers of joint inflammation in the synovial fluid of patients with anterior cruciate ligament injury. Arthritis Rheum., 2008-06-01;58(6):1707-15. 2008-06-01 [PMID: 18512776] (Human)
• Tsai HT, Wang PH, Tee YT, Lin LY, Hsieh YS, Yang SF Imbalanced serum concentration between cathepsin B and cystatin C in patients with pelvic inflammatory disease. Fertil. Steril., 2008-03-07;91(2):549-55. 2008-03-07 [PMID: 18314113] (Human)
• Potapova IA, Gaudette GR, Brink PR, Robinson RB, Rosen MR, Cohen IS, Doronin SV Mesenchymal stem cells support migration, extracellular matrix invasion, proliferation, and survival of endothelial cells in vitro. Stem Cells, 2007-03-29;25(7):1761-8. 2007-03-29 [PMID: 17395769] (Human)
• Ma DH, Yao JY, Kuo MT, See LC, Lin KY, Chen SC, Chen JK, Chao AS, Wang SF, Lin KK Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane. Invest. Ophthalmol. Vis. Sci., 2007-02-01;48(2):644-51. 2007-02-01 [PMID: 17251461] (Human)
• Rofstad EK, Mathiesen B, Kindem K, Galappathi K Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice. Cancer Res., 2006-07-01;66(13):6699-707. 2006-07-01 [PMID: 16818644] (Human)
• Miyake H, Hara I, Eto H Serum level of cathepsin B and its density in men with prostate cancer as novel markers of disease progression. Anticancer Res., 2004-07-01;24(4):2573-7. 2004-07-01 [PMID: 15330217] (Human)

FAQs for Cathepsin B (DCATB0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CTSB