Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA, 2 Plate

Lysates prepared from human VEGF R3 transfected NS0 cells (NS0-hVEGF R3) were diluted in a series and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-VEGF R3 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-VEGF R3. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGloTM Chemiluminescent Detection Substrate (Catalog # AR004). Human Phospho-VEGF R3 can be detected in this DuoSet IC ELISA by using approximately 10 to 15 times less lysate than is needed for a conventional IP-Western blot.

The indicated amounts of recombinant extracellular domains of human VEGF R3 (Catalog #349-F4), human VEGF R1 (Catalog #321-FL), human VEGF R2 (Catalog #357-KD) or human Tie-2 (Catalog #313-TI) were added to 20 μg NS0-hVEGF R3 cell lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human VEGF R3.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA, 2 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FLT4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA, 2 Plate

• EC 2.7.10
• EC 2.7.10.1
• FLT4
• Flt-4
• fms-related tyrosine kinase 4
• LMPH1A
• PCLFLT41
• soluble VEGFR3 variant 1
• soluble VEGFR3 variant 2
• soluble VEGFR3 variant 3
• Tyrosine-protein kinase receptor FLT4
• vascular endothelial growth factor receptor 3
• VEGF R3
• VEGFR3
• VEGFR-3
• VEGFR3Fms-like tyrosine kinase 4

Background

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Publications for VEGFR3/Flt-4 (DYC2724-2)(6)

Publications using DYC2724-2

• Mondal, DK;Xie, C;Buraschi, S;Iozzo, RV; Decorin suppresses tumor lymphangiogenesis: A mechanism to curtail cancer progression bioRxiv : the preprint server for biology 2023-08-29 [PMID: 37693608] (Human)
• A Dumond, C Montemagno, V Vial, R Grépin, G Pagès Anti-Vascular Endothelial Growth Factor C Antibodies Efficiently Inhibit the Growth of Experimental Clear Cell Renal Cell Carcinomas Cells, 2021-05-17;10(5):. 2021-05-17 [PMID: 34067671] (Human)
• Proteolytic activation defines distinct lymphangiogenic mechanisms for VEGFC and VEGFD J Clin Invest, 2016-05-09;0(0):. 2016-05-09 [PMID: 27159393] (Human)
• Harris , Nicole C, Davydova , Natalia, Roufail , Sally, Paquet-Fifield , Sophie, Paavonen , Karri, Karnezis , Tara, Zhang , You-Fang, Sato , Teruhiko, Rothacker , Julie, Nice , Edouard, Stacker , Steven A, Achen , Marc G The propeptides of VEGF-D determine heparin binding, receptor heterodimerization, and effects on tumor biology. J Biol Chem, 2013-02-12;288(12):8176-86. 2013-02-12 [PMID: 23404505] (Human)
• Lara Planas-Paz, Boris Strilić, Axel Goedecke, Georg Breier, Reinhard Fässler, Eckhard Lammert Mechanoinduction of lymph vessel expansion The EMBO Journal 2012-02-15 [PMID: 22157817] (Human)
• Schomber T, Zumsteg A, Strittmatter K, Crnic I, Antoniadis H, Littlewood-Evans A, Wood J, Christofori G Differential effects of the vascular endothelial growth factor receptor inhibitor PTK787/ZK222584 on tumor angiogenesis and tumor lymphangiogenesis. Mol. Cancer Ther., 2009-01-01;8(1):55-63. 2009-01-01 [PMID: 19139113] (Transgenic Mouse)

Bioinformatics

• Gene Symbol: FLT4