Human PDGF-AB Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human PDGF-AB Quantikine ELISA Kit Summary

• Background: The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves ...obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.
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• Specificity: Natural and recombinant human PDGF-AB
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human PDGF-AB Quantikine ELISA Kit

• PDGFAB
• PDGF-AB

Background

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for PDGF-AB (DHD00C)(45)

Publications using DHD00C

• Sánchez, M;Mercader Ruiz, J;Marijuán Pinel, D;Sánchez, P;Fiz, N;Guadilla, J;Azofra, J;Beitia, M;Delgado, D; Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration Scientific reports 2025-02-06 [PMID: 39915642] (Human)
• Michalak, A;Guz, M;Kozicka, J;Cybulski, M;Jeleniewicz, W;Telejko, I;Szczygie?, K;Tywanek, E;Cicho?-Lach, H; miRNAs and Hematological Markers in Non-Alcoholic Fatty Liver Disease-A New Diagnostic Path? Biomedicines 2025-01-18 [PMID: 39857813] (Human)
• Michalak, A;Guz, M;Kozicka, J;Cybulski, M;Jeleniewicz, W;Szczygie?, K;Tywanek, E;Cicho?-Lach, H; microRNAs and Other Serological Markers of Liver Fibrosis in Patients with Alcohol-Related Liver Cirrhosis Biomedicines 2024-09-16 [PMID: 39335621] (Human)
• Guo, CY;Mo, R;Kim, H; Surface topography modulates initial platelet adhesion to titanium substrata Journal of oral biology and craniofacial research 2024-06-07 [PMID: 38962718] (Human)
• Smoczer, C;Yuth, KR;Askar, MA;Young, LA;Paurazas, SB; Growth Factors Released from Advanced Platelet-Rich Fibrin in the Presence of Calcium-Based Silicate Materials and Their Impact on the Viability and Migration of Stem Cells of Apical Papilla Dentistry journal 2023-09-19 [PMID: 37754340] (Human)
• B Walters, PA Turner, B Rolauffs, ML Hart, JP Stegemann Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells Cells, 2021-11-11;10(11):. 2021-11-11 [PMID: 34831345] (Human)
• L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells Leukemia, 2021-09-23;0(0):. 2021-09-23 [PMID: 34556797] (Human)
• N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel International Journal of Molecular Sciences, 2021-03-16;22(6):. 2021-03-16 [PMID: 33809553] (Human)
• A Michalak, H Cicho?-Lac, M Guz, J Kozicka, M Cybulski, W Jeleniewic Plateletcrit and Mean Platelet Volume in the Evaluation of Alcoholic Liver Cirrhosis and Nonalcoholic Fatty Liver Disease Patients BioMed Research International, 2021-02-15;2021(0):8867985. 2021-02-15 [PMID: 33644233] (Human)
• A Michalak, H Cicho?-Lac, M Guz, J Kozicka, M Cybulski, W Jeleniewic, A Stepulak Towards an evaluation of alcoholic liver cirrhosis and nonalcoholic fatty liver disease patients with hematological scales World Journal of Gastroenterology, 2020-12-21;26(47):7538-7549. 2020-12-21 [PMID: 33384553] (Human)
• S Pauly, F Klatte-Sch, K Stahnke, M Scheibel, B Wildemann The effect of autologous platelet rich plasma on tenocytes of the human rotator cuff BMC Musculoskelet Disord, 2018-11-30;19(1):422. 2018-11-30 [PMID: 30497435] (Human)
• F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures PLoS ONE, 2018-09-06;13(9):e0203048. 2018-09-06 [PMID: 30188924] (Human)
• S Rossi, M Mori, B Vigani, MC Bonferoni, G Sandri, F Riva, C Caramella, F Ferrari A novel dressing for the combined delivery of platelet lysate and vancomycin hydrochloride to chronic skin ulcers: Hyaluronic acid particles in alginate matrices Eur J Pharm Sci, 2018-03-21;118(0):87-95. 2018-03-21 [PMID: 29574078] (Human)
• K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy Sci Rep, 2017-08-16;7(1):8484. 2017-08-16 [PMID: 28814814] (Human)
• B Shen, Z Zhang, NF Zhou, YF Huang, YJ Bao, DS Wu, YD Zhang Preparing Platelet-Rich Plasma with Whole Blood Harvested Intraoperatively During Spinal Fusion Med. Sci. Monit., 2017-07-22;23(0):3578-3584. 2017-07-22 [PMID: 28734116] (Human)
• M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro J Transl Med, 2017-05-01;15(1):90. 2017-05-01 [PMID: 28460641] (Human)
• E Tóth-Zsámb, Z Horváth, L Hajtman, S Leé, É Pállinger, E Kuklis, Á Tahy, G Fekete, L Kohut, RG Kiss Cardiac rehabilitation programme as a non-pharmacological platelet inhibitory tool in acute coronary syndrome survivors Eur J Prev Cardiol, 2017-04-25;0(0):2047487317704. 2017-04-25 [PMID: 28438028] (Human)
• Anne Fischer-Ni Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates Cytotherapy, 2016-11-22;0(0):. 2016-11-22 [PMID: 27887865] (Human)
• Gorka Orive Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts Eur. J. Obstet. Gynecol. Reprod. Biol., 2016-09-22;206(0):125-130. 2016-09-22 [PMID: 27693932] (Human)
• Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling Sci Rep, 2016-07-04;6(0):29029. 2016-07-04 [PMID: 27374240] (Human)
• Chou M, Burnouf T, Wang T Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate. PLoS ONE, 2014-06-19;9(6):e99145. 2014-06-19 [PMID: 24945500] (Human)
• Kageyama Y, Doi T, Matsushima-Nishiwaki R, Iida Y, Akamatsu S, Kondo A, Kuroyanagi G, Yamamoto N, Mizutani J, Otsuka T, Tokuda H, Iida H, Kozawa O, Ogura S Involvement of Rac in thromboxane A2induced human platelet activation: regulation of sCD40 ligand release and PDGFAB secretion. Mol Med Rep, 2014-04-15;10(1):107-12. 2014-04-15 [PMID: 24737161] (Human)
• Iudicone , Paola, Fioravanti , Daniela, Bonanno , Giuseppi, Miceli , Michelin, Lavorino , Claudio, Totta , Pierange, Frati , Luigi, Nuti , Marianna, Pierelli , Luca Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells. J Transl Med, 2014-01-27;12(0):28. 2014-01-27 [PMID: 24467837] (Human)
• Barsotti M, Losi P, Briganti E, Sanguinetti E, Magera A, Al Kayal T, Feriani R, Di Stefano R, Soldani G Effect of platelet lysate on human cells involved in different phases of wound healing. PLoS ONE, 2013-12-27;8(12):e84753. 2013-12-27 [PMID: 24386412] (Human)
• Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol, 2013-10-30;14(0):48. 2013-10-30 [PMID: 24168020] (Human)
• Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX, 2011-01-01;28(4):305-16. 2011-01-01 [PMID: 22130485] (Human)
• Ding W, Knox TR, Tschumper RC Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch. Blood, 2010-07-06;116(16):2984-93. 2010-07-06 [PMID: 20606160] (Human)
• Mathew P, Thall PF, Wen S, Bucana C, Jones D, Horne E, Oh WK, Morris MJ, Lee YC, Logothetis CJ, Lin SH, Fidler IJ Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer. Br. J. Cancer, 2008-10-07;99(9):1426-32. 2008-10-07 [PMID: 18841158] (Human)
• Kanter J, Khan SY, Kelher M, Gore L, Silliman CC Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates. Clin. Cancer Res., 2008-06-15;14(12):3942-7. 2008-06-15 [PMID: 18559616] (Human)
• Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC. Arterioscler. Thromb. Vasc. Biol., 2007-11-08;28(1):135-41. 2007-11-08 [PMID: 17991872] (Porcine)
• Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma. Int. J. Cancer, 2007-02-01;120(3):686-93. 2007-02-01 [PMID: 17096355] (Human)
• Pegorier S, Wagner LA, Gleich GJ, Pretolani M Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells. J. Immunol., 2006-10-01;177(7):4861-9. 2006-10-01 [PMID: 16982928] (Human)
• Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Clin. Cancer Res., 2006-05-01;12(9):2676-88. 2006-05-01 [PMID: 16675559] (Human)
• Zhang CQ, Yuan T, Zeng BF Experimental study of the effect of platelet-rich plasma on osteogenesis in rabbit. Chin. Med. J., 2004-12-01;117(12):1853-5. 2004-12-01 [PMID: 15603719] (Rabbit)
• Nagineni CN, Kutty V, Detrick B, Hooks JJ Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J. Cell. Physiol., 2005-04-01;203(1):35-43. 2005-04-01 [PMID: 15368539] (Human)
• Saika S, Kono-Saika S, Tanaka T, Yamanaka O, Ohnishi Y, Sato M, Muragaki Y, Ooshima A, Yoo J, Flanders KC, Roberts AB Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice. Lab. Invest., 2004-10-01;84(10):1245-58. 2004-10-01 [PMID: 15273699] (Human)
• Thorn JJ, Sorensen H, Weis-Fogh U, Andersen M Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery. Int J Oral Maxillofac Surg, 2004-01-01;33(1):95-100. 2004-01-01 [PMID: 14690664] (Human)
• Seeger FH, U£3+jV—I¬, Blessing E, Gu L, Bornhold R, Denger S, Kreuzer J Fibrinogen induces chemotactic activity in endothelial cells. 2002-10-01;176(2):109-15. 2002-10-01 [PMID: 12354170] (Porcine)
• Bracamonte MP, Rud KS, Owen WG, Miller VM Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle. Am. J. Physiol. Heart Circ. Physiol., 2002-09-01;283(3):H853-60. 2002-09-01 [PMID: 12181111] (Porcine)
• Skutek M, van Griensven M, Zeichen J, Brauer N, Bosch U Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts. Eur. J. Appl. Physiol., 2001-11-01;86(1):48-52. 2001-11-01 [PMID: 11820322] (Human)
• Westphal JR, Van't Hullenaar R, Peek R, Willems RW, Crickard K, Crickard U, Askaa J, Clemmensen I, Ruiter DJ, De Waal RM Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo. Int. J. Cancer, 2000-06-15;86(6):768-76. 2000-06-15 [PMID: 10842189] (Human)
• Landesberg R, Roy M, Glickman RS Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J. Oral Maxillofac. Surg., 2000-03-01;58(3):297. 2000-03-01 [PMID: 10716112] (Human)
• Vieweg U, 49315, Schramm J, Urbach H Platelet-derived growth factor (PDGF-AB) like immune reactivity in serum and in cerebral spinal fluid following experimental subarachnoid haemorrhage in dogs. CCN1 promotes IL-1? production in keratinocytes by activating p38 MAPK signaling in psoriasis, 1999-01-01;141(8):861-5. 1999-01-01 [PMID: 10536723] (Canine)
• Soubrier M, Sauron C, Souweine B, Larroche C, Wechsler B, Guillevin L, Piette JC, Rousset H, Deteix P Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome. Am. J. Kidney Dis., 1999-10-01;34(4):633-8. 1999-10-01 [PMID: 10516342] (Human)
• Gu JW, Adair TH Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells. Am. J. Physiol., 1997-08-01;273(2):H628-33. 1997-08-01 [PMID: 9277477] (Canine)

FAQs for PDGF-AB (DHD00C). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.