Human Oncostatin M (OSM) DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Oncostatin M (OSM) DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: OSM

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Oncostatin M (OSM) DuoSet ELISA, 15 Plate

• MGC20461
• oncostatin M
• oncostatin-M
• OSM

Background

Oncostatin M (OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM. OSM initiates its biological activities by binding to specific cell surface receptors. The gp130, a signal transducing component (beta subunit) of the IL-6, LIF and CNTF receptor complexes, was identified as a low-affinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R, a gp130-related protein) has now been identified to be a component of a high-affinity OSM receptor that will transduce OSM signals. Since OSM is also active on cells that do not express LIF R, a specific OSM receptor that does not involve LIF R must also exist. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells, as well as on other solid tumor cells, OSM also has growth stimulatory activities on normal fibroblasts, AIDS-Kaposis sarcoma cells, and a human erythroleukemia cell line, TF-1. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells, regulation of IL-6 expression in human endothelial cells, and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells.

Human Oncostatin M (OSM) signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta) and gp130 and the type II complex made up of OSM receptor beta (OSM R beta) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Human and mouse OSM R beta are 55% identical at the amino acid sequence level.

Publications for Oncostatin M/OSM (DY295)(19)

Publications using DY295

• Louault, K;Blavier, L;Lee, MH;Kennedy, RJ;Fernandez, GE;Pawel, BR;Asgharzadeh, S;DeClerck, YA; Nuclear factor-?B activation by transforming growth factor-?1 drives tumour microenvironment-mediated drug resistance in neuroblastoma British journal of cancer 2024-05-28 [PMID: 38806726] (Human)
• Adir, O;Sagi-Assif, O;Meshel, T;Ben-Menachem, S;Pasmanik-Chor, M;Hoon, DSB;Witz, IP;Izraely, S; Heterogeneity in the Metastatic Microenvironment: JunB-Expressing Microglia Cells as Potential Drivers of Melanoma Brain Metastasis Progression Cancers 2023-10-13 [PMID: 37894348] (Human)
• Kleuskens, MTA;Bek, MK;Al Halabi, Y;Blokhuis, BRJ;Diks, MAP;Haasnoot, ML;Garssen, J;Bredenoord, AJ;C A M van Esch, B;Redegeld, FA; Mast cells disrupt the function of the esophageal epithelial barrier Mucosal immunology 2023-06-09 [PMID: 37302713] ( Human)
• S Nikanfar, K Hamdi, S Haiaty, N Samadi, V Shahnazi, A Fattahi, M Nouri Oncostatin M and its receptor in women with polycystic ovary syndrome and association with assisted reproductive technology outcomes Reproductive biology, 2022-03-09;22(2):100633. 2022-03-09 [PMID: 35278823] (Human)
• MM Jones, ST Vanyo, W Ibraheem, A Maddi, MB Visser Treponema denticola stimulates Oncostatin M cytokine release and de novo synthesis in neutrophils and macrophages J. Leukoc. Biol., 2020-07-17;0(0):. 2020-07-17 [PMID: 32678942] (Human)
• D Parashar, A Geethadevi, MR Aure, J Mishra, J George, C Chen, MK Mishra, A Tahiri, W Zhao, B Nair, Y Lu, LS Mangala, C Rodriguez-, G Lopez-Bere, AKS Camara, M Liang, JS Rader, R Ramchandra, M You, AK Sood, VN Kristensen, GB Mills, S Pradeep, P Chaluvally miRNA551b-3p Activates an Oncostatin Signaling Module for the Progression of Triple-Negative Breast Cancer Cell Rep, 2019-12-24;29(13):4389-4406.e10. 2019-12-24 [PMID: 31875548] (Mouse)
• K Tawara, C Bolin, J Koncinsky, S Kadaba, H Covert, C Sutherland, L Bond, J Kronz, JR Garbow, CL Jorcyk OSM potentiates preintravasation events, increases CTC counts, and promotes breast cancer metastasis to the lung Breast Cancer Res., 2018-06-14;20(1):53. 2018-06-14 [PMID: 29898744] (Xenograft)
• K Shien, VA Papadimitr, D Ruder, C Behrens, L Shen, N Kalhor, J Song, JJ Lee, J Wang, X Tang, RS Herbst, S Toyooka, L Girard, JD Minna, JM Kurie, II Wistuba, JG Izzo JAK1/STAT3 activation through a proinflammatory cytokine pathway leads to resistance to molecularly targeted therapy in non-small cell lung cancer Mol. Cancer Ther., 2017-07-20;0(0):. 2017-07-20 [PMID: 28729401] (Human)
• JA Kucia-Tran, V Tulkki, S Smith, CG Scarpini, K Hughes, AM Araujo, KY Yan, J Botthof, E Pérez-Góme, M Quintanill, K Cuschieri, MM Caffarel, N Coleman Overexpression of the oncostatin-M receptor in cervical squamous cell carcinoma is associated with epithelial-mesenchymal transition and poor overall survival Br. J. Cancer, 2016-06-28;115(2):212-22. 2016-06-28 [PMID: 27351213] (Human)
• Su C, Chiang Y, Huang C, Hsu C, Fong Y, Tang C Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy. J Immunol, 2015-08-24;195(7):3355-64. 2015-08-24 [PMID: 26304992] (Human)
• Chen C, Su C, Huang Y, Tsai C, Fuh L, Tang C CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways. PLoS ONE, 2014-09-04;9(9):e106632. 2014-09-04 [PMID: 25187949] (Human)
• Nicolaidou V, Wong MM, Redpath AN Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. PLoS ONE, 2012-07-03;7(7):e39871. 2012-07-03 [PMID: 22802946] (Human)
• Hintzen C, Quaiser S, Pap T, Heinrich PC, Hermanns HM Induction of CCL13 expression in synovial fibroblasts highlights a significant role of oncostatin M in rheumatoid arthritis. Arthritis Rheum., 2009-07-01;60(7):1932-43. 2009-07-01 [PMID: 19565514] (Human)
• Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells. Blood, 2007-09-11;110(13):4319-30. 2007-09-11 [PMID: 17848619] (Human)
• Sugaya M, Fang L, Cardones AR, Kakinuma T, Jaber SH, Blauvelt A, Hwang ST Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes. J. Immunol., 2006-12-01;177(11):7665-72. 2006-12-01 [PMID: 17114436] (Human)
• Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression. Cancer Res., 2005-10-01;65(19):8896-904. 2005-10-01 [PMID: 16204061] (Human)
• Lopez-Karpovitch X, Barrales-Benitez O, Flores M, Piedras J Effect of azacytidine in the release of leukemia inhibitory factor, oncostatin m, interleukin (IL)-6, and IL-11 by mononuclear cells of patients with refractory anemia. Cytokine, 2002-11-24;20(4):154-62. 2002-11-24 [PMID: 12543079] (Human)
• Suda T, 146, Chida K, Todate A, Ide K, Asada K, Nakamura Y, Suzuki K, Kuwata H, Nakamura H Oncostatin M production by human dendritic cells in response to bacterial products. 2002-03-21;17(6):335-40. 2002-03-21 [PMID: 12061841] (Human)
• Savarese TM, Campbell CL, McQuain C, Mitchell K, Guardiani R, Quesenberry PJ, Nelson BE Coexpression of oncostatin M and its receptors and evidence for STAT3 activation in human ovarian carcinomas. Cytokine, 2002-03-21;17(6):324-34. 2002-03-21 [PMID: 12061840] (Human)

Bioinformatics

• Gene Symbol: OSM