Human Lymphotoxin-alpha/TNF-beta DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Lymphotoxin-alpha/TNF-beta DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: LTA

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Lymphotoxin-alpha/TNF-beta DuoSet ELISA, 15 Plate

• LT
• LTA
• LT-alpha
• lymphotoxin alpha (TNF superfamily, member 1)
• Lymphotoxin alpha
• Lymphotoxinalpha
• Lymphotoxin-alpha
• tnfb
• TNF-beta
• TNFBlymphotoxin-alpha
• TNFSF1
• TNFSF1B
• TNFSF1TNF-beta
• tumor necrosis factor beta
• Tumor necrosis factor ligand superfamily member 1

Background

Lymphotoxin alpha and Lymphotoxin beta are pro-inflammatory TNF superfamily ligands that play important roles in immune system development. LT-alpha can be secreted as a homotrimer that binds and activates TNF RI, TNF RII, HVEM, and TROY. It is required for development of the spleen, lymph nodes, and Peyer’s patches. It also regulates T cell homing to the gut and IgA induction. LT-alpha can additionally form membrane-associated heterotrimers with two copies of LT-beta/TNF-beta on the surface of B, T, LTi, and ILC3 cells. This heterotrimer binds and activates the Lymphotoxin beta R on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV). The heterotrimer promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by IEC.

Publications for Lymphotoxin-alpha/TNF-beta (DY211)(7)

Publications using DY211

• Coughlin, CA;Chahar, D;Lekakis, M;Youssfi, AA;Li, L;Roberts, E;Gallego, NC;Volmar, CH;Landgren, O;Brothers, S;Griswold, AJ;Amador, C;Bilbao, D;Maura, F;Schatz, JH; Bruton's tyrosine kinase inhibition re-sensitizes multidrug-resistant DLBCL tumors driven by BCL10 gain-of-function mutants to venetoclax Blood cancer journal 2025-02-02 [PMID: 39894894] (Human)
• Pallavi Bhatta, Kevin D. Whale, Amy K. Sawtell, Clare L. Thompson, Stephen E. Rapecki, David A. Cook et al. Bispecific antibody target pair discovery by high-throughput phenotypic screening using in vitro combinatorial Fab libraries mAbs 2021-01-25 [PMID: 33487120] (Human)
• E Simader, L Beer, M Laggner, V Vorstandle, A Gugerell, M Erb, P Kalinina, D Copic, D Moser, A Spittler, E Tschachler, H Jan Ankers, M Mildner Tissue-regenerative potential of the secretome of gamma-irradiated peripheral blood mononuclear cells is mediated via TNFRSF1B-induced necroptosis Cell Death Dis, 2019-09-30;10(10):729. 2019-09-30 [PMID: 31570701] (Human)
• C Zhang, J Zhan, M Zhao, H Dai, Y Deng, W Zhou, L Zhao Protective mechanism of Taxifolin for chlorpyrifos neurotoxicity in BV2 cells Neurotoxicology, 2019-05-29;74(0):74-80. 2019-05-29 [PMID: 31152760] (Mouse)
• CC Tsai, YJ Lin, HR Yu, JM Sheen, IC Lin, YJ Lai, YL Tain, LT Huang, MM Tiao Regulation of Leptin Methylation Not via Apoptosis by Melatonin in the Rescue of Chronic Programming Liver Steatosis Int J Mol Sci, 2018-11-12;19(11):. 2018-11-12 [PMID: 30424542] (Rat)
• Sarhan D, Palma M, Mao Y, Adamson L, Kiessling R, Mellstedt H, Osterborg A, Lundqvist A Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta. Eur J Immunol, 2015-04-17;45(6):1783-93. 2015-04-17 [PMID: 25773885] (Human)
• Correale J, Farez M, Razzitte G Helminth infections associated with multiple sclerosis induce regulatory B cells. Ann. Neurol., 2008-08-01;64(2):187-99. 2008-08-01 [PMID: 18655096] (Human)

FAQs for Lymphotoxin-alpha/TNF-beta (DY211). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LTA