Human IL-18 BPa Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-18 BPa Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-18 BPa in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-18 BPa. Results obtain...ed using natural human IL-18 BPa showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 BPa.
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• Specificity: Natural and recombinant human IL-18 BPa
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL18BP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-18 BPa Quantikine ELISA Kit

• IL18 BPa
• IL-18 BPa
• IL18BP
• IL-18BP
• IL18BPa
• interleukin 18 binding protein
• interleukin-18-binding protein
• MC51L-53L-54L homolog gene product
• tadekinig-alfa

Background

Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-18 BPa/IL18BP (DBP180)(13)

Publications using DBP180

• Meshko, B;Volatier, TLA;Mann, J;Kluth, MA;Ganss, C;Frank, MH;Frank, NY;Ksander, BR;Cursiefen, C;Notara, M; Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population International journal of molecular sciences 2024-09-07 [PMID: 39273646] (Human)
• Nabatanzi, R;Ssekamatte, P;Castelnuovo, B;Kambugu, A;Nakanjako, D; Increased Levels of Caspase-1 and IL-1? Among Adults With Persistent Immune Activation After 12 Years of Suppressive Antiretroviral Therapy in the Infectious Diseases Institute HIV Treatment Cohort Open forum infectious diseases 2023-11-01 [PMID: 37953818] (Human)
• Dai, B;Cao, H;Hu, Y;Gong, Z;Huang, X;Chen, Y;Liu, F;Peng, X;Zhang, Y;Lei, X; Role of NLRP3 inflammasome activation in HCC cell progression Heliyon 2023-09-01 [PMID: 37681160] (Human)
• W Cui, L Hull, A Zizzo, L Wang, B Lin, M Zhai, M Xiao Pharmacokinetic Study of rhIL-18BP and Its Effect on Radiation-Induced Cytokine Changes in Mouse Serum and Intestine Toxics, 2022-12-30;11(1):. 2022-12-30 [PMID: 36668761] (Mouse)
• HA Al-Rawaf, AH Alghadir, SA Gabr Expression of Circulating MicroRNAs and Myokines and Interactions with Serum Osteopontin in Type 2 Diabetic Patients with Moderate and Poor Glycemic Control: A Biochemical and Molecular Study BioMed Research International, 2021-12-07;2021(0):7453000. 2021-12-07 [PMID: 34917685] (Human)
• Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males. Antioxidants (Basel), 2021-05-28;10(6):. 2021-05-28 [PMID: 34071378] (Human)
• E Wlodek, RB Kirkpatric, S Andrews, R Noble, R Schroyer, J Scott, CJE Watson, M Clatworthy, EM Harrison, SJ Wigmore, K Stevenson, D Kingsmore, NS Sheerin, O Bestard, HA Stirnadel-, L Abberley, M Busz, S DeWall, M Birchler, D Krull, KS Thorneloe, A Weber, L Devey A pilot study evaluating GSK1070806 inhibition of interleukin-18 in renal transplant delayed graft function PLoS ONE, 2021-03-08;16(3):e0247972. 2021-03-08 [PMID: 33684160] (Human)
• T Zhou, W Damsky, OE Weizman, MK McGeary, KP Hartmann, CE Rosen, S Fischer, R Jackson, RA Flavell, J Wang, MF Sanmamed, MW Bosenberg, AM Ring IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy Nature, 2020-06-24;0(0):. 2020-06-24 [PMID: 32581358] (Human)
• A Hedbrant, L Andersson, IL Bryngelsso, D Eklund, H Westberg, E Särndahl, A Persson Quartz Dust Exposure Affects NLRP3 Inflammasome Activation and Plasma Levels of IL-18 and IL-1Ra in Iron Foundry Workers Mediators Inflamm., 2020-01-07;2020(0):8490908. 2020-01-07 [PMID: 32256196] (Human)
• A Federico, M Dallio, M Masarone, AG Gravina, R Di Sarno, C Tuccillo, V Cossiga, S Lama, P Stiuso, F Morisco, M Persico, C Loguercio Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters, and Serological Worsening Markers in Nonalcoholic Fatty Liver Disease Patients Oxid Med Cell Longev, 2019;2019(0):8742075. 2019 [PMID: 31737175] (Human)
• TAT Tran, HW Grievink, K Lipinska, C Kluft, J Burggraaf, M Moerland, D Tasev, KE Malone Whole blood assay as a model for in vitro evaluation of inflammasome activation and subsequent caspase-mediated interleukin-1 beta release PLoS ONE, 2019-04-08;14(4):e0214999. 2019-04-08 [PMID: 30958862] (Human)
• YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease J Transl Med, 2017-05-31;15(1):121. 2017-05-31 [PMID: 28569153] (Human)
• Sundstrom P, Ahlmanner F, Akeus P, Sundquist M, Alsen S, Yrlid U, Borjesson L, Sjoling A, Gustavsson B, Wong S, Quiding-Jarbrink M Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-gamma. J Immunol, 2015-08-21;195(7):3472-81. 2015-08-21 [PMID: 26297765] (Human)

FAQs for IL-18 BPa/IL18BP (DBP180). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL18BP