Human IL-12/IL-23 p40 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-12/IL-23 p40 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL12B

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-12/IL-23 p40 DuoSet ELISA, 15 Plate

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Publications for IL-12/IL-23 p40 (DY1240)(31)

Publications using DY1240

• K Shabir, S Gharanei, S Orton, V Patel, P Chauhan, E Karteris, HS Randeva, JE Brown, I Kyrou Asprosin Exerts Pro-Inflammatory Effects in THP-1 Macrophages Mediated via the Toll-like Receptor 4 (TLR4) Pathway International Journal of Molecular Sciences, 2022-12-23;24(1):. 2022-12-23 [PMID: 36613673] (Human)
• LAJ Feather, V Nadella, E Kastner, Y Perrie, AC Hilton, A Devitt Development of a rapid in vitro pre-screen for distinguishing effective liposome-adjuvant delivery systems Scientific Reports, 2022-07-20;12(1):12448. 2022-07-20 [PMID: 35859154] (Human)
• B van der Lu, MCP Vos, M Grootte Br, N Ijssennagg, F Vrieling, J Meijerink, WT Steegenga The effects of sulfated secondary bile acids on intestinal barrier function and immune response in an inflammatory in vitro human intestinal model Heliyon, 2022-02-02;8(2):e08883. 2022-02-02 [PMID: 35169646] (Human)
• T Katopodi, S Petanidis, K Domvri, P Zarogoulid, D Anestakis, C Charalampi, D Tsavlis, C Bai, H Huang, L Freitag, W Hohenforst, D Matthaios, K Porpodis Kras-driven intratumoral heterogeneity triggers infiltration of M2 polarized macrophages via the circHIPK3/PTK2 immunosuppressive circuit Scientific Reports, 2021-07-29;11(1):15455. 2021-07-29 [PMID: 34326381] (Human)
• AL Martel, NL Fraleigh, E Picard, JD Lewicky, G Pawelec, H Lee, GW Ma, L Mousavifar, R Roy, HT Le Novel immunomodulatory properties of low dose cytarabine entrapped in a mannosylated cationic liposome International journal of pharmaceutics, 2021-07-01;0(0):120849. 2021-07-01 [PMID: 34216770] (Human)
• S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist Stem Cells, 2019-05-13;0(0):. 2019-05-13 [PMID: 31002437] (Human)
• L Su, P Pan, P Yan, Y Long, X Zhou, X Wang, R Zhou, B Wen, L Xie, D Liu Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis Sci Rep, 2019-04-05;9(1):5747. 2019-04-05 [PMID: 30952998] (Human)
• C Wei, C Yang, S Wang, D Shi, C Zhang, X Lin, Q Liu, R Dou, B Xiong Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis Mol. Cancer, 2019-03-30;18(1):64. 2019-03-30 [PMID: 30927925] (Human)
• A Islam, I Urbarova, JA Bruun, I Martinez-Z Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells Eur Cell Mater, 2019-02-20;37(0):153-174. 2019-02-20 [PMID: 30785213] (Human)
• MH Kubala, V Punj, VR Placencio-, H Fang, GE Fernandez, R Sposto, YA DeClerck Plasminogen Activator Inhibitor-1 Promotes the Recruitment and Polarization of Macrophages in Cancer Cell Rep, 2018-11-20;25(8):2177-2191.e7. 2018-11-20 [PMID: 30463014] (Human)
• NM Soares, JN de Souza, TF Leal, EAG Reis, MS Miranda, WLC Dos Santos, MCA Teixeira Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-? Production by Macrophages In Vitro Biomed Res Int, 2017-11-05;2017(0):5861453. 2017-11-05 [PMID: 29230410] (Human)
• T Nakajima, H Yoshifuji, M Shimizu, K Kitagori, K Murakami, R Nakashima, Y Imura, M Tanaka, K Ohmura, F Matsuda, C Terao, T Mimori A novel susceptibility locus in the IL12B region is associated with the pathophysiology of Takayasu arteritis through IL-12p40 and IL-12p70 production Arthritis Res. Ther., 2017-09-06;19(1):197. 2017-09-06 [PMID: 28874185] (Human)
• AB Guimarães-, NC Rochael, F Oliveira, J Echevarria, EM Saraiva Neutrophil Extracellular Traps Reprogram IL-4/GM-CSF-Induced Monocyte Differentiation to Anti-inflammatory Macrophages Front Immunol, 2017-05-17;8(0):523. 2017-05-17 [PMID: 28567039] (Human)
• NS Hojo-Souza, DB Pereira, FS de Souza, TA de Oliveir, MS Cardoso, MS Tada, GM Zanini, DC Bartholome, RT Fujiwara, LL Bueno On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease Malar. J, 2017-01-24;16(1):42. 2017-01-24 [PMID: 28118834] (Human)
• Raundhal M, Morse C, Khare A, Oriss T, Milosevic J, Trudeau J, Huff R, Pilewski J, Holguin F, Kolls J, Wenzel S, Ray P, Ray A High IFN-gamma and low SLPI mark severe asthma in mice and humans. J Clin Invest, 2015-06-29;125(8):3037-50. 2015-06-29 [PMID: 26121748]
• Fleming B, Chandrasekaran P, Dillon L, Dalby E, Suresh R, Sarkar A, El-Sayed N, Mosser D The generation of macrophages with anti-inflammatory activity in the absence of STAT6 signaling. J Leukoc Biol, 2015-06-05;98(3):395-407. 2015-06-05 [PMID: 26048978] (Human)
• Jaguin M, Fardel O, Lecureur V Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2. PLoS ONE, 2015-02-24;10(2):e0116560. 2015-02-24 [PMID: 25710172] (Human)
• Guirado E, Mbawuike U, Keiser T, Arcos J, Azad A, Wang S, Schlesinger L Characterization of host and microbial determinants in individuals with latent tuberculosis infection using a human granuloma model. MBio, 2015-02-17;6(1):e02537-14. 2015-02-17 [PMID: 25691598] (Human)
• Wu J, Hsu H, Chiu Y, Chen H, Ni Y, Chang M The effects of cytokines on spontaneous hepatitis B surface antigen seroconversion in chronic hepatitis B virus infection. J Immunol, 2014-12-12;194(2):690-6. 2014-12-12 [PMID: 25505294] (Human)
• Madan-Lala R, Sia J, King R, Adekambi T, Monin L, Khader S, Pulendran B, Rengarajan J Mycobacterium tuberculosis impairs dendritic cell functions through the serine hydrolase Hip1. J Immunol, 2014-03-21;192(9):4263-72. 2014-03-21 [PMID: 24659689] (Human)
• Slavica L, Nordstrom I, Karlsson M, Valadi H, Kacerovsky M, Jacobsson B, Eriksson K TLR3 impairment in human newborns. J Leukoc Biol, 2013-07-30;94(5):1003-11. 2013-07-30 [PMID: 23901120] (Human)
• Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. J. Immunol., 2009-12-15;183(12):8176-85. 2009-12-15 [PMID: 20007583] (Human)
• Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009-03-02;119(10):1424-32. 2009-03-02 [PMID: 19255340] (Human)
• Lamhamedi-Cherradi SE, Martin RE, Ito T, Kheradmand F, Corry DB, Liu YJ, Moyle M Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12. J. Immunol., 2008-05-01;180(9):6000-9. 2008-05-01 [PMID: 18424720] (Human)
• Lauzon NM, Mian F, MacKenzie R, Ashkar AA The direct effects of Toll-like receptor ligands on human NK cell cytokine production and cytotoxicity. Cell. Immunol., 2006-10-17;241(2):102-12. 2006-10-17 [PMID: 17049504] (Human)
• Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CW, Izumi Y, Taubman MA B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease. Am. J. Pathol., 2006-09-01;169(3):987-98. 2006-09-01 [PMID: 16936272] (Human)
• Cho YJ, Ahn BY, Lee NG, Lee DH, Kim DS A combination of E. coli DNA fragments and modified lipopolysaccharides as a cancer immunotherapy. Vaccine, 2006-05-06;24(31):5862-71. 2006-05-06 [PMID: 16725239] (Human)
• Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T, Heine H, Brandt E, Reiling N The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation. Blood, 2006-04-06;108(3):965-73. 2006-04-06 [PMID: 16601243] (Human)
• Anderson ST, Williams AJ, Brown JR, Newton SM, Simsova M, Nicol MP, Sebo P, Levin M, Wilkinson RJ, Wilkinson KA Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing. Am. J. Respir. Crit. Care Med., 2006-02-02;173(9):1038-42. 2006-02-02 [PMID: 16456140] (Human)
• Fritz JH, Girardin SE, Fitting C, Werts C, Mengin-Lecreulx D, Caroff M, Cavaillon JM, Philpott DJ, Adib-Conquy M Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists. Eur. J. Immunol., 2005-08-01;35(8):2459-70. 2005-08-01 [PMID: 16021602] (Human)
• Asselin-Paturel C, Brizard G, Pin JJ, Briere F, Trinchieri G Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody. J. Immunol., 2003-12-15;171(12):6466-77. 2003-12-15 [PMID: 14662846] (Mouse)

FAQs for IL-12/IL-23 p40 (DY1240). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL12B