Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit Summary

• Background: The QuantiGlo Human IL-1 beta Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure human IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant protein. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural ...IL-1 beta showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.
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• Specificity: Natural and recombinant human IL-1 beta. This assay also recognizes natural and recombinant rhesus macaque IL-1 beta. This assay cross-reacts 15.4% with recombinant Human Pro-IL-1 beta , 1.0% with recombinant Rat IL-1 beta , 0.6% with recombinant Cotton Rat IL-1 beta .
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL1B

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit

• Catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Publications for IL-1 beta/IL-1F2 (QLB00B)(12)

Publications using QLB00B

• Mucientes, A;Lisbona-Montañez, JM;Mena-Vázquez, N;Ruiz-Limón, P;Manrique-Arija, S;García-Studer, A;Ortiz-Márquez, F;Fernández-Nebro, A; Intestinal Dysbiosis, Tight Junction Proteins, and Inflammation in Rheumatoid Arthritis Patients: A Cross-Sectional Study International journal of molecular sciences 2024-08-08 [PMID: 39201334] (Human)
• C Ge, J Tan, X Dai, Q Kuang, S Zhong, L Lai, C Yi, Y Sun, J Luo, C Zhang, L Zhu, B Wang, M Xu Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK Oncogene, 2022-10-08;13(1):5945. 2022-10-08 [PMID: 36209205] (Human)
• DQ Song, J Liu, F Wang, XF Li, MH Liu, Z Zhang, SS Cao, X Jiang Procyanidin�B2 inhibits lipopolysaccharide?induced apoptosis by suppressing the Bcl?2/Bax and NF?&kappaB signalling pathways in human umbilical vein endothelial cells Molecular Medicine Reports, 2021-02-12;23(4):1. 2021-02-12 [PMID: 33576443] (Human)
• X Shao, S Guha, W Lu, KE Campagno, JM Beckel, JA Mills, W Yang, CH Mitchell Polarized Cytokine Release Triggered by P2X7 Receptor from Retinal Pigmented Epithelial Cells Dependent on Calcium Influx Cells, 2020-11-24;9(12):. 2020-11-24 [PMID: 33255431] (Human)
• S Yuan, C Tang, D Chen, F Li, M Huang, J Ye, Z He, W Li, Y Chen, X Lin, X Wang, X Cai miR-98 Modulates Cytokine Production from Human PBMCs in Systemic Lupus Erythematosus by Targeting IL-6 mRNA J Immunol Res, 2019-12-01;2019(0):9827574. 2019-12-01 [PMID: 31886314] (Human)
• M Egawa, S Haze, Y Gozu, J Hosoi, T Onodera, Y Tojo, M Katsuyama, Y Hara, C Katagiri, N Inoue, S Furukawa, G Suzuki Evaluation of psychological stress in confined environments using salivary, skin, and facial image parameters Sci Rep, 2018-05-29;8(1):8264. 2018-05-29 [PMID: 29844534] (Human)
• A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women Oncotarget, 2017-09-15;8(54):92134-92142. 2017-09-15 [PMID: 29190903] (Human)
• Cifuentes M, Fuentes C, Tobar N, Acevedo I, Villalobos E, Hugo E, Ben-Jonathan N, Reyes M Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue. Mol Cell Endocrinol, 2012-03-16;361(1):24-30. 2012-03-16 [PMID: 22449852] (Human)
• Fichorova RN, Richardson-Harman N, Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Curtis K, Dezzutti CS, Donoval B, Doncel GF, Donaghay M, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pasicznyk JM, Pallansch-Cokonis M, Poli G, Reichelderfer P, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R, Cummins JE Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study. Anal. Chem., 2008-05-17;80(12):4741-51. 2008-05-17 [PMID: 18484740] (Human)
• Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Br. J. Pharmacol., 2007-03-12;151(1):115-27. 2007-03-12 [PMID: 17351655] (Human)
• Dahaba AA, Elawady GA, Rehak PH, List WF Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients. Anaesth Intensive Care, 2002-06-01;30(3):269-74. 2002-06-01 [PMID: 12075632] (Human)
• MacKenzie A, Wilson HL, Kiss-Toth E, Dower SK, North RA, Surprenant A Rapid secretion of interleukin-1beta by microvesicle shedding. Immunity, 2001-11-01;15(5):825-35. 2001-11-01 [PMID: 11728343] (Human)

Bioinformatics

• Gene Symbol: IL1B