Human IL-1 alpha/IL-1F1 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-1 alpha/IL-1F1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL1A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1 alpha/IL-1F1 DuoSet ELISA, 15 Plate

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

Publications for IL-1 alpha/IL-1F1 (DY200)(40)

Publications using DY200

• Ruan, YH;Ning, WR;Huang, AQ;Jiang, D;Zhu, BX;Liu, XC;Gong, JL;Huang, K;Kuang, DM;Wu, Y;Zheng, L; Intracellular Interleukin-1? in Peritumoral Monocytes Induces IL8 Production and Inhibits Mitophagy to Promote Stemness and Metastasis of Hepatocellular Carcinoma Cancer research 2025-08-26 [PMID: 40857615] (Human)
• Ye, S;Lee, S;Kang, S;Jun, SH;Kang, NG; Improvement of Skin Condition Through RXR Alpha-Activating Materials Biomolecules 2025-02-17 [PMID: 40001599] (Human)
• Pourfarzam, S;Ardestani, SK;Jamali, T;Ghazanfari, H;Naghizadeh, MM;Faghihzadeh, S;Yaraee, R;Ghazanfari, Z;Ghazanfari, T; Distinct inflammatory profiles in mustard lung: A study of sulfur mustard-exposed patients with serious pulmonary complications International immunopharmacology 2024-12-16 [PMID: 39689604] (Human)
• Klein, R;Onyuru, J;Viera, EM;Putnam, CD;Hoffman, HM;Hastings, ML; Modulating NLRP3 splicing with antisense oligonucleotides to control pathological inflammation bioRxiv : the preprint server for biology 2024-09-06 [PMID: 39282364] (Human)
• Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M; Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls PloS one 2024-07-15 [PMID: 39008499] (Human)
• Atasoy-Zeybek, A;Hawse, GP;Nagelli, CV;De Padilla, CL;Abdel, MP;Evans, CH; Transcriptomic changes during the replicative senescence of human articular chondrocytes bioRxiv : the preprint server for biology 2023-11-07 [PMID: 37986862] (Human)
• Zobel, CM;Wenzel, W;Krüger, JP;Baumgarten, U;Wagelöhner, T;Neumann, N;Foroutan, B;Müller, R;Müller, A;Rauschning, D;Schü beta ler, M;Scheit, L;Weinreich, F;Oltmanns, K;Keidel, F;Koch, M;Spethmann, S;Schreiner, M; Serum interleukin-6, procalcitonin, and C-reactive protein at hospital admission can identify patients at low risk for severe COVID-19 progression Frontiers in microbiology 2023-10-23 [PMID: 37937220] (Human)
• Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D; Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial PloS one 2023-11-02 [PMID: 37917760] (Human)
• Membrane-bound Interleukin-1 alpha mediates leukocyte adhesion during atherogenesis [PMID: 37701434] (Human)
• YY Chao, A Puhach, D Frieser, M Arunkumar, L Lehner, T Seeholzer, A Garcia-Lop, M van der Wa, S Fibi-Smeta, A Dietschman, T Sommermann, T ?ikovi?, L Taher, MS Gresnigt, SJ Vastert, F van Wijk, G Panagiotou, D Krappmann, O Gro beta , CE Zielinski Human TH17 cells engage gasdermin E pores to release IL-1alpha on NLRP3 inflammasome activation Nature Immunology, 2023-01-05;0(0):. 2023-01-05 [PMID: 36604548] (Human)
• H Chen, ME Diolaiti, PC O'Leary, A Rojc, NJ Krogan, M Kim, A Ashworth A whole genome CRISPR screen identifies AHR loss as a mechanism of PARP7 inhibitor resistance Molecular Cancer Therapeutics, 2022-07-05;0(0):. 2022-07-05 [PMID: 35439318] (Human)
• SC Lean, RL Jones, SA Roberts, AEP Heazell A prospective cohort study providing insights for markers of adverse pregnancy outcome in older mothers BMC pregnancy and childbirth, 2021-10-20;21(1):706. 2021-10-20 [PMID: 34670515] (Human)
• SH Kim, SH Hong, JH Lee, DH Lee, K Jung, JY Yang, HS Shin, J Lee, J Jeong, JH Oh Skin Sensitization Evaluation of Carbon-Based Graphene Nanoplatelets Toxics, 2021-03-17;9(3):. 2021-03-17 [PMID: 33803047] (Human)
• K Bernau, JP Leet, H Floerke, EM Bruhn, AL Noll, IS McDermott, S Esnault, NN Jarjour, N Sandbo Interleukin-1&alpha Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation Cells, 2021-03-02;10(3):. 2021-03-02 [PMID: 33801398] (Human)
• SH Jun, H Kim, H Lee, JE Song, SG Park, NG Kang Synthesis of Retinol-Loaded Lipid Nanocarrier via Vacuum Emulsification to Improve Topical Skin Delivery Polymers, 2021-03-08;13(5):. 2021-03-08 [PMID: 33800335] (Human)
• T Takemoto, R Kaetsu, M Hanayama, Y Ishiyama, M Sadamura, K Nishio, M Tsunoda, M Asano, M Motoyoshi Acid-electrolyzed functional water-induces Interleukin-1&alpha release from Intracellular Storage Sites in Oral Squamous Cell Carcinoma International Journal of Medical Sciences, 2021-02-18;18(8):1746-1752. 2021-02-18 [PMID: 33746591] (Human)
• S Niklander, D Bandaru, DW Lambert, KD Hunter ROCK inhibition modulates the senescence-associated secretory phenotype (SASP) in oral keratinocytes FEBS Open Bio, 2020-11-06;0(0):. 2020-11-06 [PMID: 33095981] (Human)
• S Tumpara, B Martinez-D, G Gomez-Mari, B Liu, DS DeLuca, E Korenbaum, D Jonigk, F Jugert, FM Wurm, MJ Wurm, T Welte, S Janciauski The Delivery of &alpha1-Antitrypsin Therapy Through Transepidermal Route: Worthwhile to Explore Front Pharmacol, 2020-07-03;11(0):983. 2020-07-03 [PMID: 32719598] (Human)
• A Aimond, K Calabro, C Audoin, E Olivier, M Dutot, P Buron, P Rat, O Laprévote, S Prado, E Roulland, OP Thomas, G Genta-Jouv Cytotoxic and Anti-Inflammatory Effects of Ent-Kaurane Derivatives Isolated from the Alpine Plant Sideritis hyssopifolia Molecules, 2020-01-29;25(3):. 2020-01-29 [PMID: 32013210] (Human)
• S Saito, M Ohtsu, M Asano, T Ishigami Ouabain signaling in oral squamous cell carcinoma cells J Oral Sci, 2019-09-20;0(0):. 2019-09-20 [PMID: 31548455] (Human)
• X Jiang, F Wang, Y Wang, A Gisterå, J Roy, G Paulsson-B, U Hedin, A Lerman, GK Hansson, J Herrmann, ZQ Yan Inflammasome-Driven Interleukin-1α and�Interleukin-1? Production in Atherosclerotic Plaques Relates to Hyperlipidemia and Plaque Complexity JACC Basic Transl Sci, 2019-06-24;4(3):304-317. 2019-06-24 [PMID: 31312755] (Human)
• M Ma, CM Percopo, DE Sturdevant, AC Sek, HD Komarow, HF Rosenberg Cytokine Diversity in Human Peripheral Blood Eosinophils: Profound Variability of IL-16 J. Immunol., 2019-06-10;0(0):. 2019-06-10 [PMID: 31182481] (Human)
• S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist Stem Cells, 2019-05-13;0(0):. 2019-05-13 [PMID: 31002437] (Human)
• R Saga, K Hasegawa, K Murata, M Chiba, T Nakamura, K Okumura, E Tsuruga, Y Hosokawa Regulation of radiosensitivity by 4-methylumbelliferone via the suppression of interleukin-1 in fibrosarcoma cells Oncol Lett, 2019-01-29;17(3):3555-3561. 2019-01-29 [PMID: 30867797] (Human)
• M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal Cell Rep, 2018-12-26;25(13):3706-3720.e8. 2018-12-26 [PMID: 30590043] (Human)
• CC Sheth, RM López-Pedr, MDM Jovani-San, R González-M, V Veses Modulation of salivary cytokines in response to alcohol, tobacco and caffeine consumption: a pilot study Sci Rep, 2018-11-12;8(1):16687. 2018-11-12 [PMID: 30420770] (Human)
• M Kusunoki, E Sata, K Nishio, T Tanaka, T Nishida, N Sugano, S Sato, M Asano Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines Int J Med Sci, 2018-09-07;15(12):1365-1372. 2018-09-07 [PMID: 30275765] (Human)
• RF Hamilton, Z Wu, S Mitra, A Holian The Effects of Varying Degree of MWCNT Carboxylation on Bioactivity in Various In Vivo and In Vitro Exposure Models Int J Mol Sci, 2018-01-25;19(2):. 2018-01-25 [PMID: 29370073] (Human)
• B Lagrange, S Benaoudia, P Wallet, F Magnotti, A Provost, F Michal, A Martin, F Di Lorenzo, BF Py, A Molinaro, T Henry Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11 Nat Commun, 2018-01-16;9(1):242. 2018-01-16 [PMID: 29339744] (Human)
• E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD Respir. Res., 2017-08-22;18(1):159. 2017-08-22 [PMID: 28830544] (Human)
• LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton Production and regulation of interleukin-1 family cytokines at the materno-fetal interface Cytokine, 2017-07-13;0(0):. 2017-07-13 [PMID: 28712670] (Human)
• GR Kathamuthu, K Moideen, D Baskaran, VV Banurekha, D Nair, G Sekar, R Sridhar, B Vidyajayan, G Gajendrara, DK Parandhama, A Srinivasan, S Babu TB lymphadenitis is associated with enhanced baseline and antigen - specific induction of Type 1 and Type 17 cytokines and reduced IL-1? and IL-18 at the site of infection Clin. Vaccine Immunol, 2017-05-05;0(0):. 2017-05-05 [PMID: 28249842] (Human)
• Bortezomib treatment sensitizes oncolytic HSV-1 treated tumors to NK cell immunotherapy Clin Cancer Res, 2016-07-07;0(0):. 2016-07-07 [PMID: 27390350] (Mouse)
• Simanski M, Rademacher F, Schroder L, Glaser R, Harder J The Inflammasome and the Epidermal Growth Factor Receptor (EGFR) Are Involved in the Staphylococcus aureus-Mediated Induction of IL-1alpha and IL-1beta in Human Keratinocytes. PLoS ONE, 2016-01-25;11(1):e0147118. 2016-01-25 [PMID: 26808616] (Human)
• Haddad L, Sirois M Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils. PLoS ONE, 2014-02-12;9(2):e88980. 2014-02-12 [PMID: 24563688] (Human)
• Polyak M, Vivithanaporn P, Maingat F, Walsh J, Branton W, Cohen E, Meeker R, Power C Differential type 1 interferon-regulated gene expression in the brain during AIDS: interactions with viral diversity and neurovirulence. FASEB J, 2013-04-22;27(7):2829-44. 2013-04-22 [PMID: 23608145] (Human)
• Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J. Exp. Med., 2012-07-16;209(8):1505-17. 2012-07-16 [PMID: 22802353] (Human)
• Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J. Clin. Invest., 2007-12-01;117(12):3664-3672. 2007-12-01 [PMID: 17992260] (Human)
• Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1). Cytokine, 2005-07-21;31(2):153-60. 2005-07-21 [PMID: 15923123] (Human)
• Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha. Circ. Res., 2003-08-28;93(6):515-22. 2003-08-28 [PMID: 12946945] (Human)

Review for IL-1 alpha/IL-1F1 (DY200) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Patricia Carulla Martí 06/22/2023

Summary

• Sample Tested: Cultured Human Keratinocytes,Supernatant Human Epidermal keratinocytes (HEKa) in presence or absence of Poly(I:C)
• Lot: P309019

FAQs for IL-1 alpha/IL-1F1 (DY200). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL1A