Human IFN-beta Quantikine ELISA Kit

IFN-beta is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 98% compared to 55% for the top competitor.

Samples containing high concentrations of IFN-beta in various matrices are diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 103%-112% compared to 119%-170% for the top competitor.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IFN-beta Quantikine ELISA Kit Summary

• Background: The Quantikine® Human IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-beta in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using t...he Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta .
  Show More
• Specificity: Natural and recombinant human IFN-beta .
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IFN-beta Quantikine ELISA Kit

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IFN-beta (DIFNB0)(28)

Publications using DIFNB0

• Zhang, J;Zhang, L;Liu, D;Shi, H;Zhang, X;Chen, J;Yang, X;Zeng, M;Zhang, J;Feng, T;Zhu, X;Jing, Z;Ji, Z;Shi, D;Feng, L; Helicase protein DDX11 as a novel antiviral factor promoting RIG-I-MAVS-mediated signaling pathway mBio 2024-10-29 [PMID: 39470258] (Human)
• Choi, JH;Luo, J;Hesketh, GG;Guo, S;Pistofidis, A;Ladak, RJ;An, Y;Naeli, P;Alain, T;Schmeing, TM;Gingras, AC;Duchaine, T;Zhang, X;Sonenberg, N;Jafarnejad, SM; Repression of mRNA translation initiation by GIGYF1 via disrupting the eIF3-eIF4G1 interaction Science advances 2024-07-19 [PMID: 39018414] (Human)
• Callon, D;Glenet, M;Lebreil, AL;Heng, L;Bouland, N;Fichel, C;Fornes, P;Andreoletti, L;Berri, F; Major Group-B Enterovirus populations deleted in the noncoding 5' region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis PLoS pathogens 2024-05-02 [PMID: 38696536] (Mouse)
• Liu, X;Tang, J;Wang, Z;Zhu, C;Deng, H;Sun, X;Yu, G;Rong, F;Chen, X;Liao, Q;Jia, S;Liu, W;Zha, H;Fan, S;Cai, X;Gui, JF;Xiao, W; Oxygen enhances antiviral innate immunity through maintenance of EGLN1-catalyzed proline hydroxylation of IRF3 Nature communications 2024-04-26 [PMID: 38670937] (Human)
• Araya, R;Men, S;Uekusa, Y;Yu, Z;Kikuchi, H;Daitoku, K;Minakawa, M;Kawaguchi, S;Furukawa, KI;Oshima, Y;Imaizumi, T;Seya, K; The inhibitory effect of DIF-3 on polyinosinic-polycytidylic acid-induced innate immunity activation in human cerebral microvascular endothelial cells Journal of pharmacological sciences 2024-03-01 [PMID: 38395516] (Human)
• Reuschl, AK;Thorne, LG;Whelan, MVX;Ragazzini, R;Furnon, W;Cowton, VM;De Lorenzo, G;Mesner, D;Turner, JLE;Dowgier, G;Bogoda, N;Bonfanti, P;Palmarini, M;Patel, AH;Jolly, C;Towers, GJ; Evolution of enhanced innate immune suppression by SARS-CoV-2 Omicron subvariants Nature microbiology 2024-01-16 [PMID: 38228858] (Human)
• Murayama, T;Nakayama, J;Jiang, X;Miyata, K;Morris, AD;Cai, KQ;Prasad, RM;Ma, X;Efimov, A;Belani, N;Gerstein, ER;Tan, Y;Zhou, Y;Kim, W;Maruyama, R;Campbell, KS;Chen, L;Yang, Y;Balachandran, S;Canadas, I; Targeting DHX9 triggers tumor-intrinsic interferon response and replication stress in Small Cell Lung Cancer Cancer discovery 2024-01-08 [PMID: 38189443] (Human)
• A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill Macrophage fumarate hydratase restrains mtRNA-mediated interferon production Nature, 2023-03-08;615(7952):490-498. 2023-03-08 [PMID: 36890227] (Human)
• JY Li, Y Zhao, S Gong, MM Wang, X Liu, QM He, YQ Li, SY Huang, H Qiao, XR Tan, ML Ye, XH Zhu, SW He, Q Li, YL Liang, KL Chen, SW Huang, QJ Li, J Ma, N Liu TRIM21 inhibits irradiation-induced mitochondrial DNA release and impairs antitumour immunity in nasopharyngeal carcinoma tumour models Nature Communications, 2023-02-16;14(1):865. 2023-02-16 [PMID: 36797289] (Human)
• J Nassour, LG Aguiar, A Correia, TT Schmidt, L Mainz, S Przetocka, C Haggblom, N Tadepalle, A Williams, MN Shokhirev, SC Akincilar, V Tergaonkar, GS Shadel, J Karlseder Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis Nature, 2023-02-08;614(7949):767-773. 2023-02-08 [PMID: 36755096] (Human)
• TK Kim, S Jeon, S Park, SK Sonn, S Seo, J Suh, J Jin, HY Kweon, S Kim, SH Moon, O Kweon, BH Koo, N Kim, HO Lee, YM Kim, YJ Kim, SH Park, GT Oh 2'-5' oligoadenylate synthetase?like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability Nature Communications, 2022-11-04;13(1):6647. 2022-11-04 [PMID: 36333342] (Human, Mouse)
• CH Song, YH Kim, M Naskar, BW Hayes, MA Abraham, JH Noh, G Suk, MJ Kim, KS Cho, M Shin, EJ Lee, SN Abraham, HW Choi Lactobacillus crispatus Limits Bladder Uropathogenic E. coli Infection by Triggering a Host Type I Interferon Response Proceedings of the National Academy of Sciences of the United States of America, 2022-08-08;119(33):e2117904119. 2022-08-08 [PMID: 35939684] (Human)
• C Song, D Liu, S Liu, D Li, I Horecny, X Zhang, P Li, L Chen, M Miller, R Chowdhury, M Issa, R Shen, Y Yan, F Zhang, L Zhang, L Zhang, C Bai, J Feng, L Zhuang, R Zhang, J Li, H Wilkinson, J Liu, W Tao SHR1032, a novel STING agonist, stimulates anti-tumor immunity and directly induces AML apoptosis Scientific Reports, 2022-05-20;12(1):8579. 2022-05-20 [PMID: 35595822] (Cynomolgus Monkey, Human)
• KC Huang, D Chanda, S McGrath, V Dixit, C Zhang, J Wu, K Tendyke, H Yao, R Hukkanen, N Taylor, D Verbel, DS Kim, A Endo, TA Noland, Y Chen, M Matijevic, J Wang, J Hutz, N Sarwar, FG Fang, X Bao Pharmacological Activation of STING in Bladder Induces Potent Anti-tumor Immunity in Non-Muscle Invasive Murine Bladder Cancer Molecular Cancer Therapeutics, 2022-06-01;0(0):. 2022-06-01 [PMID: 35313332] (Mouse)
• Z Gao, X Zhang, L Zhang, S Wu, J Ma, F Wang, Y Zhou, X Dai, E Bullitt, Y Du, JT Guo, J Chang A yellow fever virus NS4B inhibitor not only suppresses viral replication, but also enhances the virus activation of RIG-I-like receptor-mediated innate immune response PloS Pathogens, 2022-01-21;18(1):e1010271. 2022-01-21 [PMID: 35061864] (Human)
• R Ishida, J Cole, J Lopez-Oroz, N Fayad, A Felix-Lope, M Elaish, SY Luo, O Julien, A Kumar, TC Hobman Mayaro Virus Non-Structural Protein 2 Circumvents the Induction of Interferon in Part by Depleting Host Transcription Initiation Factor IIE Subunit 2 Cells, 2021-12-12;10(12):. 2021-12-12 [PMID: 34944018] (Human)
• AH Pham, J Mitchell, S Botto, KM Pryke, VR DeFilippis, MH Hancock Human cytomegalovirus blocks canonical TGFbeta signaling during lytic infection to limit induction of type I interferons PloS Pathogens, 2021-08-19;17(8):e1009380. 2021-08-19 [PMID: 34411201] (Human)
• U Rand, SY Kupke, H Shkarlet, MD Hein, T Hirsch, P Marichal-G, L Cicin-Sain, U Reichl, D Bruder Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate Immunity Cells, 2021-07-11;10(7):. 2021-07-11 [PMID: 34359926] (Human)
• Y Li, C Bax, J Patel, T Vazquez, A Ravishanka, MM Bashir, M Grinnell, D Diaz, VP Werth Plasma-derived DNA containing-extracellular vesicles induce STING-mediated proinflammatory responses in dermatomyositis Theranostics, 2021-05-21;11(15):7144-7158. 2021-05-21 [PMID: 34158841] (Human)
• G Miglietta, M Russo, RC Duardo, G Capranico G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells Nucleic Acids Research, 2021-07-09;0(0):. 2021-07-09 [PMID: 34139015] (Human)
• S Al-Beltagi, CA Preda, LV Goulding, J James, J Pu, P Skinner, Z Jiang, BL Wang, J Yang, AC Banyard, KH Mellits, P Gershkovic, CJ Hayes, J Nguyen-Van, IH Brown, J Liu, KC Chang Thapsigargin Is a Broad-Spectrum Inhibitor of Major Human Respiratory Viruses: Coronavirus, Respiratory Syncytial Virus and Influenza A Virus Viruses, 2021-02-03;13(2):. 2021-02-03 [PMID: 33546185] (Human)
• K Yu, H Tian, H Deng PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasion Sci Adv, 2020-11-20;6(47):. 2020-11-20 [PMID: 33219031] (Human)
• HJ Hurley, H Dewald, ZS Rothkopf, S Singh, F Jenkins, P Deb, S De, BJ Barnes, P Fitzgerald Frontline Science: AMPK regulates metabolic reprogramming necessary for interferon production in human plasmacytoid dendritic cells J. Leukoc. Biol., 2020-07-08;0(0):. 2020-07-08 [PMID: 32640499] (Human)
• JH Seo, CS Hwang, JY Yoo MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection Sci Rep, 2020-07-07;10(1):11183. 2020-07-07 [PMID: 32636430] (Human)
• SF Watson, N Bellora, S Macias ILF3 contributes to the establishment of the antiviral type I interferon program Nucleic Acids Res., 2020-01-10;0(0):. 2020-01-10 [PMID: 31701124] (Human)
• Y Wang, S Yuan, X Jia, Y Ge, T Ling, M Nie, X Lan, S Chen, A Xu Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS Nat. Cell Biol., 2019-11-04;21(11):1346-1356. 2019-11-04 [PMID: 31685995] (Mouse)
• Q Zhao, Y Wei, SJ Pandol, L Li, A Habtezion STING Signaling Promotes Inflammation in Experimental Acute Pancreatitis Gastroenterology, 2018-02-06;0(0):. 2018-02-06 [PMID: 29425920] (Human)
• H Mori, M Murakami, T Tsuda, K Kameda, R Utsunomiya, K Masuda, K Shiraishi, X Dai, M Tohyama, H Nakaoka, K Sayama Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytes J. Dermatol. Sci., 2018;0(0):. 2018 [PMID: 29395576] (ELISA Capture, Human)

FAQs for IFN-beta (DIFNB0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.