Human IFN-beta DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IFN-beta DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IFN-beta DuoSet ELISA, 5 Plate

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Publications for IFN-beta (DY814-05)(44)

Publications using DY814-05

• García, M;Carrasco García, A;Weigel, W;Christ, W;Lira-Junior, R;Wirth, L;Tauriainen, J;Maleki, K;Vanoni, G;Vaheri, A;Mäkelä, S;Mustonen, J;Nordgren, J;Smed-Sörensen, A;Strandin, T;Mjösberg, J;Klingström, J; Innate lymphoid cells are activated in HFRS, and their function can be modulated by hantavirus-induced type I interferons PLoS pathogens 2024-07-01 [PMID: 39038044] (Human)
• Akalu, YT;Patel, RS;Taft, J;Canas-Arranz, R;Richardson, A;Buta, S;Martin-Fernandez, M;Sazeides, C;Pearl, RL;Mainkar, G;Kurland, AP;Geltman, R;Rosberger, H;Kang, DD;Kurian, AA;Kaur, K;Altman, J;Dong, Y;Johnson, JR;Zhangi, L;Lim, JK;Albrecht, RA;García-Sastre, A;Rosenberg, BR;Bogunovic, D; Broad-spectrum RNA antiviral inspired by ISG15 -/- deficiency bioRxiv : the preprint server for biology 2024-06-28 [PMID: 38979204] (Human)
• McGee, JE;Kirsch, JR;Kenney, D;Cerbo, F;Chavez, EC;Shih, TY;Douam, F;Wong, WW;Grinstaff, MW; Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potency Nature biotechnology 2024-07-08 [PMID: 38977924] (Human)
• Jauregui, GV;Vuki?, D;Onyango, IG;Arias, C;Novotný, JS;Texlová, K;Wang, S;Kova?ovicova, KL;Polakova, N;Zelinkova, J;?arna, M;Strašil, VL;Head, BP;Havas, D;Mistrik, M;Zorec, R;Verkhratsky, A;Keegan, L;O'Connel, M;Rissman, R;Stokin, GB; Amyloid precursor protein induces reactive astrogliosis bioRxiv : the preprint server for biology 2023-12-18 [PMID: 38187544] (Mouse)
• Zobel, CM;Wenzel, W;Krüger, JP;Baumgarten, U;Wagelöhner, T;Neumann, N;Foroutan, B;Müller, R;Müller, A;Rauschning, D;Schü beta ler, M;Scheit, L;Weinreich, F;Oltmanns, K;Keidel, F;Koch, M;Spethmann, S;Schreiner, M; Serum interleukin-6, procalcitonin, and C-reactive protein at hospital admission can identify patients at low risk for severe COVID-19 progression Frontiers in microbiology 2023-10-23 [PMID: 37937220] (Human)
• Dai, J;Agbemabiese, CA;Griffin, AN;Patton, JT; Rotavirus capping enzyme VP3 inhibits interferon expression by inducing MAVS degradation during viral replication mBio 2023-10-31 [PMID: 37905816] (Human)
• Wang, X;Wang, Y;Cao, A;Luo, Q;Chen, D;Zhao, W;Xu, J;Li, Q;Bu, X;Quan, J; Development of cyclopeptide inhibitors of cGAS targeting protein-DNA interaction and phase separation Nature communications 2023-10-02 [PMID: 37783727] (Human)
• Imamura, R;Sato, H;Chih-Cheng Voon, D;Shirasaki, T;Honda, M;Kurachi, M;Sakai, K;Matsumoto, K; Met receptor is essential for MAVS-mediated antiviral innate immunity in epithelial cells independent of its kinase activity Proceedings of the National Academy of Sciences of the United States of America 2023-10-03 [PMID: 37748074] (Human)
• McGee, JE;Kirsch, JR;Kenney, D;Chavez, E;Shih, TY;Douam, F;Wong, WW;Grinstaff, MW; Complete substitution with modified nucleotides suppresses the early interferon response and increases the potency of self-amplifying RNA bioRxiv : the preprint server for biology 2023-09-17 [PMID: 37745375] (Human)
• Schutt, WR;Conde, JN;Mladinich, MC;Himmler, GE;Mackow, ER; ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFN?/? expression and promotes ZIKV persistence mBio 2023-09-14 [PMID: 37707056] (Human)
• Barasa, L;Chaudhuri, S;Zhou, JY;Jiang, Z;Choudhary, S;Green, RM;Wiggin, E;Cameron, M;Humphries, F;Fitzgerald, KA;Thompson, PR; Development of LB244, an Irreversible STING Antagonist Journal of the American Chemical Society 2023-09-20 [PMID: 37695732] (Human)
• Kuruppu, H;Wickramanayake, WPRH;Jeewandara, C;Peranantharajah, D;Colambage, HS;Perera, L;Gomes, L;Wijewickrama, A;Ogg, GS;Malavige, GN; Adipokine levels and their association with clinical disease severity in patients with dengue PLoS neglected tropical diseases 2023-09-01 [PMID: 37676889] (Human)
• Twarog, M;Schustak, J;Xu, Y;Coble, M;Dolan, K;Esterberg, R;Huang, Q;Saint-Geniez, M;Bao, Y; TNF? induced by DNA-sensing in macrophage compromises retinal pigment epithelial (RPE) barrier function Scientific reports 2023-09-02 [PMID: 37660150] (Human)
• Neumair, J;Kröger, M;Stütz, E;Jerin, C;Chaker, AM;Schmidt-Weber, CB;Seidel, M; Flow-Based CL-SMIA for the Quantification of Protein Biomarkers from Nasal Secretions in Comparison with Sandwich ELISA Biosensors 2023-06-22 [PMID: 37504069] (Human)
• É Lacasse, L Gudimard, I Dubuc, A Gravel, I Allaeys, É Boilard, L Flamand SARS-CoV-2 Nsp2 Contributes to Inflammation by Activating NF-kappaB Viruses, 2023-01-24;15(2):. 2023-01-24 [PMID: 36851549] (Human)
• SY Bando, FB Bertonha, SE Vieira, DBL de Oliveir, VN Chalup, EL Durigon, P Palmeira, ACP Curi, CS Faria, L Antonangel, GDP Lauterbach, FA Regalio, RM Cesar, CA Moreira-Fi Blood leukocyte transcriptional modules and differentially expressed genes associated with disease severity and age in COVID-19 patients Scientific Reports, 2023-01-17;13(1):898. 2023-01-17 [PMID: 36650374] (Human)
• W Alturaiki, H Alkadi, S Alamri, ME Awadalla, A Alfaez, A Mubarak, MA Alanazi, FQ Alenzi, BF Flanagan, B Alosaimi Association between the expression of Toll-like receptors, cytokines, and homeostatic chemokines in SARS-CoV-2 infection and COVID-19 severity Heliyon, 2022-12-24;0(0):e12653. 2022-12-24 [PMID: 36589720] (Human)
• K Motani, N Saito-Tara, K Nishino, S Yamauchi, N Minakawa, H Kosako The Golgi-resident protein ACBD3 concentrates STING at ER-Golgi contact sites to drive export from the ER Cell Reports, 2022-12-20;41(12):111868. 2022-12-20 [PMID: 36543137] (Human)
• X Liu, M Wang, M Yang, H Sun, B Wang, X Pan, X Chen, J Jin, X Wang Structure-activity relationship study of amidobenzimidazole derivatives as stimulator of interferon genes (STING) agonists European Journal of Medicinal Chemistry, 2022-11-24;246(0):114943. 2022-11-24 [PMID: 36462438] (Human)
• D Kabelitz, M Zarobkiewi, M Heib, R Serrano, M Kunz, G Chitadze, D Adam, C Peters Signal strength of STING activation determines cytokine plasticity and cell death in human monocytes Scientific Reports, 2022-10-24;12(1):17827. 2022-10-24 [PMID: 36280676] (Human)
• J Hong, JH Lee, Z Zhang, Y Wu, M Yang, Y Liao, R de la Rosa, J Scheirer, D Pechacek, N Zhang, Z Xu, T Curiel, X Tan, TH Huang, K Xu PRC2-mediated epigenetic suppression of type I IFN-STAT2 signaling impairs antitumor immunity in luminal breast cancer Cancer Research, 2022-12-16;0(0):. 2022-12-16 [PMID: 36222718] (Human)
• AA Hinay, S Kakee, S Kageyama, A Tsuneki-To, WY Perdana, Y Akena, S Nishiyama, K Kanai Pro-Inflammatory Cytokines and Interferon-Stimulated Gene Responses Induced by Seasonal Influenza A Virus with Varying Growth Capabilities in Human Lung Epithelial Cell Lines Vaccines, 2022-09-09;10(9):. 2022-09-09 [PMID: 36146585] (Human)
• Z Qin, X Fang, W Sun, Z Ma, T Dai, S Wang, Z Zong, H Huang, H Ru, H Lu, B Yang, S Lin, F Zhou, L Zhang Deactylation by SIRT1 enables liquid-liquid phase separation of IRF3/IRF7 in innate antiviral immunity Nature Immunology, 2022-07-25;0(0):. 2022-07-25 [PMID: 35879450] (Human)
• H Stölting, L Baillon, R Frise, K Bonner, RJ Hewitt, PL Molyneaux, ML Gore, Breathing, WS Barclay, S Saglani, CM Lloyd Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1 Mucosal Immunology, 2022-07-15;0(0):. 2022-07-15 [PMID: 35840680] (Human)
• S Shruthi, JM Sibi, V Mohan, S Babu, V Nirmaladev, V Aravindhan Decreased leukocyte exhaustion is associated with decreased IFN-beta and increased alpha-defensin-1 levels in type-2 diabetes Cytokine, 2022-05-28;156(0):155918. 2022-05-28 [PMID: 35640417] (Human)
• V Artusa, C Ciaramelli, A D'Aloia, FA Facchini, N Gotri, A Bruno, B Costa, A Palmioli, C Airoldi, F Peri Green and Roasted Coffee Extracts Inhibit Interferon-beta Release in LPS-Stimulated Human Macrophages Frontiers in Pharmacology, 2022-05-05;13(0):806010. 2022-05-05 [PMID: 35600887] (Human)
• Alexis R. Gibson, Adam Sateriale, Jennifer E. Dumaine, Julie B. Engiles, Ryan D. Pardy, Jodi A. Gullicksrud et al. A genetic screen identifies a protective type III interferon response to Cryptosporidium that requires TLR3 dependent recognition PLOS Pathogens 2022-05-18 [PMID: 35584177] (Mouse)
• T Sahr, P Escoll, C Rusniok, S Bui, G Pehau-Arna, G Lavieu, C Buchrieser Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs targeting the host immune response Nature Communications, 2022-02-09;13(1):762. 2022-02-09 [PMID: 35140216] (Human)
• HR Jung, S Jo, MJ Jeon, H Lee, Y Chu, J Lee, E Kim, GY Song, C Jung, H Kim, S Lee Development of Small-Molecule STING Activators for Cancer Immunotherapy Biomedicines, 2021-12-24;10(1):. 2021-12-24 [PMID: 35052713] (Human)
• K Nishioka, T Daidoji, T Nakaya Downregulation of calcium-regulated heat stable protein 1 expression by low-temperature stimulation causes reduction of interferon-beta expression and sensitivity to influenza viral infection Virus research, 2021-12-17;0(0):198659. 2021-12-17 [PMID: 34929215] (Human)
• FL Pennemann, A Mussabekov, C Urban, A Stukalov, LL Andersen, V Grass, TM Lavacca, C Holze, L Oubraham, Y Benamrouch, E Girardi, RE Boulos, R Hartmann, G Superti-Fu, M Habjan, JL Imler, C Meignin, A Pichlmair Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators Nature Communications, 2021-12-01;12(1):7009. 2021-12-01 [PMID: 34853303] (Human)
• J Steinberg, T Wadenpohl, S Jung The Endogenous RIG-I Ligand Is Generated in Influenza A-Virus Infected Cells Viruses, 2021-08-07;13(8):. 2021-08-07 [PMID: 34452429] (Human)
• T Ban, M Kikuchi, GR Sato, A Manabe, N Tagata, K Harita, A Nishiyama, K Nishimura, R Yoshimi, Y Kirino, H Yanai, Y Matsumoto, S Suzuki, H Hihara, M Ito, K Tsukahara, K Yoshimatsu, T Yamamoto, T Taniguchi, H Nakajima, S Ito, T Tamura Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease Nature Communications, 2021-07-19;12(1):4379. 2021-07-19 [PMID: 34282144] (Human)
• DP Strange, B Jiyarom, H Sadri-Arde, LH Cazares, TA Kenny, MD Ward, S Verma Paracrine IFN Response Limits ZIKV Infection in Human Sertoli Cells Frontiers in Microbiology, 2021-05-17;12(0):667146. 2021-05-17 [PMID: 34079533] (Human)
• MR Hendricks, S Lane, JA Melvin, Y Ouyang, DB Stolz, JV Williams, Y Sadovsky, JM Bomberger Extracellular vesicles promote transkingdom nutrient transfer during viral-bacterial co-infection Cell Reports, 2021-01-26;34(4):108672. 2021-01-26 [PMID: 33503419] (Human)
• G Ni, Z Ma, JP Wong, Z Zhang, E Cousins, MB Major, B Damania PPP6C Negatively Regulates STING-Dependent Innate Immune Responses MBio, 2020-08-04;11(4):. 2020-08-04 [PMID: 32753499] (Human)
• M Glenet, Y N'Guyen, A Mirand, C Henquell, AL Lebreil, F Berri, F Bani-Sadr, B Lina, I Schuffenec, L Andreolett Major 5'terminally deleted enterovirus populations modulate type I IFN response in acute myocarditis patients and in human cultured cardiomyocytes Sci Rep, 2020-07-20;10(1):11947. 2020-07-20 [PMID: 32686697] (Human)
• M Sermershei, AD Kenney, PH Lin, TM McMichael, C Cai, K Gumpper, TMA Adesanya, H Li, X Zhou, KH Park, JS Yount, J Ma MG53 suppresses interferon-&beta and inflammation via regulation of ryanodine receptor-mediated intracellular calcium signaling Nat Commun, 2020-07-17;11(1):3624. 2020-07-17 [PMID: 32681036] (Human)
• DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells MBio, 2019-07-16;10(4):. 2019-07-16 [PMID: 31311882] (Human)
• H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway Elife, 2019-02-08;8(0):. 2019-02-08 [PMID: 30735121] (Human)
• P An, MT Sáenz Robl, AM Duray, PG Cantalupo, JM Pipas Human polyomavirus BKV infection of endothelial cells results in interferon pathway induction and persistence PLoS Pathog., 2019-01-08;15(1):e1007505. 2019-01-08 [PMID: 30620752] (Human)
• L Strzadala, A Fiedorowic, E Wysokinska, E Ziolo, M Grudzie?, M Jelen, K Pluta, B Morak-Mlod, M Zimecki, W Kalas An Anti-Inflammatory Azaphenothiazine Inhibits Interferon ? Expression and CXCL10 Production in KERTr Cells Molecules, 2018-09-24;23(10):. 2018-09-24 [PMID: 30250011] (Human)
• C Petes, V Mintsopoul, RL Finnen, BW Banfield, K Gee The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages J. Biol. Chem., 2018-09-21;0(0):. 2018-09-21 [PMID: 30242126] (Human)
• MC Mladinich, J Schwedes, ER Mackow Zika Virus Persistently Infects and Is Basolaterally Released from Primary Human Brain Microvascular Endothelial Cells MBio, 2017-07-11;8(4):. 2017-07-11 [PMID: 28698279] (Human)