Human IFN-beta DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IFN-beta DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IFN-beta DuoSet ELISA, 5 Plate

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Publications for IFN-beta (DY814-05)(48)

Publications using DY814-05

• Constant, DA;Van Winkle, JA;Norwood, PA;Mishra, G;Meyer, KA;Laney, ME;Madden, SR;Griffith, A;Rodrigues, PF;Savan, R;Nice, TJ; Plasmacytoid dendritic cells in the intestine preferentially produce interferon lambda at homeostasis contributing to tonic localized innate immune responses bioRxiv : the preprint server for biology 2025-08-28 [PMID: 40909490] (Human)
• Ghaffarpour, S;Ghazanfari, T;Ardestani, SK;Naghizadeh, MM;Vaez Mahdavi, MR;Salehi, M;Majd, AMM;Rashidi, A;Chenary, MR;Mostafazadeh, A;Rezaei, A;Khodadadi, A;Iranparast, S;Khazaei, HA; Cytokine profiles dynamics in COVID-19 patients: a longitudinal analysis of disease severity and outcomes Scientific reports 2025-04-23 [PMID: 40269030] (Human)
• Himmler, GE;Mladinich, MC;Conde, JN;Gorbunova, EE;Lindner, MR;Kim, HK;Mackow, ER; Passage-attenuated Powassan virus LI9P protects mice from lethal LI9 challenge and links envelope residue D308 to neurovirulence mBio 2025-02-25 [PMID: 39998203] (Human)
• Hee, JR;Cheng, D;Chen, YH;Wang, SH;Chao, CH;Huang, SW;Ling, P;Wan, SW;Chang, CP;Chu, JJH;Yeh, TM;Wang, JR; A non-structural protein 1 substitution of dengue virus enhances viral replication by interfering with the antiviral signaling pathway Journal of biomedical science 2025-02-20 [PMID: 39972477] (Human)
• García, M;Carrasco García, A;Weigel, W;Christ, W;Lira-Junior, R;Wirth, L;Tauriainen, J;Maleki, K;Vanoni, G;Vaheri, A;Mäkelä, S;Mustonen, J;Nordgren, J;Smed-Sörensen, A;Strandin, T;Mjösberg, J;Klingström, J; Innate lymphoid cells are activated in HFRS, and their function can be modulated by hantavirus-induced type I interferons PLoS pathogens 2024-07-01 [PMID: 39038044] (Human)
• Akalu, YT;Patel, RS;Taft, J;Canas-Arranz, R;Richardson, A;Buta, S;Martin-Fernandez, M;Sazeides, C;Pearl, RL;Mainkar, G;Kurland, AP;Geltman, R;Rosberger, H;Kang, DD;Kurian, AA;Kaur, K;Altman, J;Dong, Y;Johnson, JR;Zhangi, L;Lim, JK;Albrecht, RA;García-Sastre, A;Rosenberg, BR;Bogunovic, D; Broad-spectrum RNA antiviral inspired by ISG15 -/- deficiency bioRxiv : the preprint server for biology 2024-06-28 [PMID: 38979204] (Human)
• McGee, JE;Kirsch, JR;Kenney, D;Cerbo, F;Chavez, EC;Shih, TY;Douam, F;Wong, WW;Grinstaff, MW; Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potency Nature biotechnology 2024-07-08 [PMID: 38977924] (Human)
• Jauregui, GV;Vuki?, D;Onyango, IG;Arias, C;Novotný, JS;Texlová, K;Wang, S;Kova?ovicova, KL;Polakova, N;Zelinkova, J;?arna, M;Strašil, VL;Head, BP;Havas, D;Mistrik, M;Zorec, R;Verkhratsky, A;Keegan, L;O'Connel, M;Rissman, R;Stokin, GB; Amyloid precursor protein induces reactive astrogliosis bioRxiv : the preprint server for biology 2023-12-18 [PMID: 38187544] (Mouse)
• Zobel, CM;Wenzel, W;Krüger, JP;Baumgarten, U;Wagelöhner, T;Neumann, N;Foroutan, B;Müller, R;Müller, A;Rauschning, D;Schü beta ler, M;Scheit, L;Weinreich, F;Oltmanns, K;Keidel, F;Koch, M;Spethmann, S;Schreiner, M; Serum interleukin-6, procalcitonin, and C-reactive protein at hospital admission can identify patients at low risk for severe COVID-19 progression Frontiers in microbiology 2023-10-23 [PMID: 37937220] (Human)
• Dai, J;Agbemabiese, CA;Griffin, AN;Patton, JT; Rotavirus capping enzyme VP3 inhibits interferon expression by inducing MAVS degradation during viral replication mBio 2023-10-31 [PMID: 37905816] (Human)
• Wang, X;Wang, Y;Cao, A;Luo, Q;Chen, D;Zhao, W;Xu, J;Li, Q;Bu, X;Quan, J; Development of cyclopeptide inhibitors of cGAS targeting protein-DNA interaction and phase separation Nature communications 2023-10-02 [PMID: 37783727] (Human)
• Imamura, R;Sato, H;Chih-Cheng Voon, D;Shirasaki, T;Honda, M;Kurachi, M;Sakai, K;Matsumoto, K; Met receptor is essential for MAVS-mediated antiviral innate immunity in epithelial cells independent of its kinase activity Proceedings of the National Academy of Sciences of the United States of America 2023-10-03 [PMID: 37748074] (Human)
• McGee, JE;Kirsch, JR;Kenney, D;Chavez, E;Shih, TY;Douam, F;Wong, WW;Grinstaff, MW; Complete substitution with modified nucleotides suppresses the early interferon response and increases the potency of self-amplifying RNA bioRxiv : the preprint server for biology 2023-09-17 [PMID: 37745375] (Human)
• Schutt, WR;Conde, JN;Mladinich, MC;Himmler, GE;Mackow, ER; ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFN?/? expression and promotes ZIKV persistence mBio 2023-09-14 [PMID: 37707056] (Human)
• Barasa, L;Chaudhuri, S;Zhou, JY;Jiang, Z;Choudhary, S;Green, RM;Wiggin, E;Cameron, M;Humphries, F;Fitzgerald, KA;Thompson, PR; Development of LB244, an Irreversible STING Antagonist Journal of the American Chemical Society 2023-09-20 [PMID: 37695732] (Human)
• Kuruppu, H;Wickramanayake, WPRH;Jeewandara, C;Peranantharajah, D;Colambage, HS;Perera, L;Gomes, L;Wijewickrama, A;Ogg, GS;Malavige, GN; Adipokine levels and their association with clinical disease severity in patients with dengue PLoS neglected tropical diseases 2023-09-01 [PMID: 37676889] (Human)
• Twarog, M;Schustak, J;Xu, Y;Coble, M;Dolan, K;Esterberg, R;Huang, Q;Saint-Geniez, M;Bao, Y; TNF? induced by DNA-sensing in macrophage compromises retinal pigment epithelial (RPE) barrier function Scientific reports 2023-09-02 [PMID: 37660150] (Human)
• Neumair, J;Kröger, M;Stütz, E;Jerin, C;Chaker, AM;Schmidt-Weber, CB;Seidel, M; Flow-Based CL-SMIA for the Quantification of Protein Biomarkers from Nasal Secretions in Comparison with Sandwich ELISA Biosensors 2023-06-22 [PMID: 37504069] (Human)
• É Lacasse, L Gudimard, I Dubuc, A Gravel, I Allaeys, É Boilard, L Flamand SARS-CoV-2 Nsp2 Contributes to Inflammation by Activating NF-kappaB Viruses, 2023-01-24;15(2):. 2023-01-24 [PMID: 36851549] (Human)
• SY Bando, FB Bertonha, SE Vieira, DBL de Oliveir, VN Chalup, EL Durigon, P Palmeira, ACP Curi, CS Faria, L Antonangel, GDP Lauterbach, FA Regalio, RM Cesar, CA Moreira-Fi Blood leukocyte transcriptional modules and differentially expressed genes associated with disease severity and age in COVID-19 patients Scientific Reports, 2023-01-17;13(1):898. 2023-01-17 [PMID: 36650374] (Human)
• W Alturaiki, H Alkadi, S Alamri, ME Awadalla, A Alfaez, A Mubarak, MA Alanazi, FQ Alenzi, BF Flanagan, B Alosaimi Association between the expression of Toll-like receptors, cytokines, and homeostatic chemokines in SARS-CoV-2 infection and COVID-19 severity Heliyon, 2022-12-24;0(0):e12653. 2022-12-24 [PMID: 36589720] (Human)
• K Motani, N Saito-Tara, K Nishino, S Yamauchi, N Minakawa, H Kosako The Golgi-resident protein ACBD3 concentrates STING at ER-Golgi contact sites to drive export from the ER Cell Reports, 2022-12-20;41(12):111868. 2022-12-20 [PMID: 36543137] (Human)
• X Liu, M Wang, M Yang, H Sun, B Wang, X Pan, X Chen, J Jin, X Wang Structure-activity relationship study of amidobenzimidazole derivatives as stimulator of interferon genes (STING) agonists European Journal of Medicinal Chemistry, 2022-11-24;246(0):114943. 2022-11-24 [PMID: 36462438] (Human)
• D Kabelitz, M Zarobkiewi, M Heib, R Serrano, M Kunz, G Chitadze, D Adam, C Peters Signal strength of STING activation determines cytokine plasticity and cell death in human monocytes Scientific Reports, 2022-10-24;12(1):17827. 2022-10-24 [PMID: 36280676] (Human)
• J Hong, JH Lee, Z Zhang, Y Wu, M Yang, Y Liao, R de la Rosa, J Scheirer, D Pechacek, N Zhang, Z Xu, T Curiel, X Tan, TH Huang, K Xu PRC2-mediated epigenetic suppression of type I IFN-STAT2 signaling impairs antitumor immunity in luminal breast cancer Cancer Research, 2022-12-16;0(0):. 2022-12-16 [PMID: 36222718] (Human)
• AA Hinay, S Kakee, S Kageyama, A Tsuneki-To, WY Perdana, Y Akena, S Nishiyama, K Kanai Pro-Inflammatory Cytokines and Interferon-Stimulated Gene Responses Induced by Seasonal Influenza A Virus with Varying Growth Capabilities in Human Lung Epithelial Cell Lines Vaccines, 2022-09-09;10(9):. 2022-09-09 [PMID: 36146585] (Human)
• Z Qin, X Fang, W Sun, Z Ma, T Dai, S Wang, Z Zong, H Huang, H Ru, H Lu, B Yang, S Lin, F Zhou, L Zhang Deactylation by SIRT1 enables liquid-liquid phase separation of IRF3/IRF7 in innate antiviral immunity Nature Immunology, 2022-07-25;0(0):. 2022-07-25 [PMID: 35879450] (Human)
• H Stölting, L Baillon, R Frise, K Bonner, RJ Hewitt, PL Molyneaux, ML Gore, Breathing, WS Barclay, S Saglani, CM Lloyd Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1 Mucosal Immunology, 2022-07-15;0(0):. 2022-07-15 [PMID: 35840680] (Human)
• S Shruthi, JM Sibi, V Mohan, S Babu, V Nirmaladev, V Aravindhan Decreased leukocyte exhaustion is associated with decreased IFN-beta and increased alpha-defensin-1 levels in type-2 diabetes Cytokine, 2022-05-28;156(0):155918. 2022-05-28 [PMID: 35640417] (Human)
• V Artusa, C Ciaramelli, A D'Aloia, FA Facchini, N Gotri, A Bruno, B Costa, A Palmioli, C Airoldi, F Peri Green and Roasted Coffee Extracts Inhibit Interferon-beta Release in LPS-Stimulated Human Macrophages Frontiers in Pharmacology, 2022-05-05;13(0):806010. 2022-05-05 [PMID: 35600887] (Human)
• Alexis R. Gibson, Adam Sateriale, Jennifer E. Dumaine, Julie B. Engiles, Ryan D. Pardy, Jodi A. Gullicksrud et al. A genetic screen identifies a protective type III interferon response to Cryptosporidium that requires TLR3 dependent recognition PLOS Pathogens 2022-05-18 [PMID: 35584177] (Mouse)
• T Sahr, P Escoll, C Rusniok, S Bui, G Pehau-Arna, G Lavieu, C Buchrieser Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs targeting the host immune response Nature Communications, 2022-02-09;13(1):762. 2022-02-09 [PMID: 35140216] (Human)
• HR Jung, S Jo, MJ Jeon, H Lee, Y Chu, J Lee, E Kim, GY Song, C Jung, H Kim, S Lee Development of Small-Molecule STING Activators for Cancer Immunotherapy Biomedicines, 2021-12-24;10(1):. 2021-12-24 [PMID: 35052713] (Human)
• K Nishioka, T Daidoji, T Nakaya Downregulation of calcium-regulated heat stable protein 1 expression by low-temperature stimulation causes reduction of interferon-beta expression and sensitivity to influenza viral infection Virus research, 2021-12-17;0(0):198659. 2021-12-17 [PMID: 34929215] (Human)
• FL Pennemann, A Mussabekov, C Urban, A Stukalov, LL Andersen, V Grass, TM Lavacca, C Holze, L Oubraham, Y Benamrouch, E Girardi, RE Boulos, R Hartmann, G Superti-Fu, M Habjan, JL Imler, C Meignin, A Pichlmair Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators Nature Communications, 2021-12-01;12(1):7009. 2021-12-01 [PMID: 34853303] (Human)
• J Steinberg, T Wadenpohl, S Jung The Endogenous RIG-I Ligand Is Generated in Influenza A-Virus Infected Cells Viruses, 2021-08-07;13(8):. 2021-08-07 [PMID: 34452429] (Human)
• T Ban, M Kikuchi, GR Sato, A Manabe, N Tagata, K Harita, A Nishiyama, K Nishimura, R Yoshimi, Y Kirino, H Yanai, Y Matsumoto, S Suzuki, H Hihara, M Ito, K Tsukahara, K Yoshimatsu, T Yamamoto, T Taniguchi, H Nakajima, S Ito, T Tamura Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease Nature Communications, 2021-07-19;12(1):4379. 2021-07-19 [PMID: 34282144] (Human)
• DP Strange, B Jiyarom, H Sadri-Arde, LH Cazares, TA Kenny, MD Ward, S Verma Paracrine IFN Response Limits ZIKV Infection in Human Sertoli Cells Frontiers in Microbiology, 2021-05-17;12(0):667146. 2021-05-17 [PMID: 34079533] (Human)
• MR Hendricks, S Lane, JA Melvin, Y Ouyang, DB Stolz, JV Williams, Y Sadovsky, JM Bomberger Extracellular vesicles promote transkingdom nutrient transfer during viral-bacterial co-infection Cell Reports, 2021-01-26;34(4):108672. 2021-01-26 [PMID: 33503419] (Human)
• G Ni, Z Ma, JP Wong, Z Zhang, E Cousins, MB Major, B Damania PPP6C Negatively Regulates STING-Dependent Innate Immune Responses MBio, 2020-08-04;11(4):. 2020-08-04 [PMID: 32753499] (Human)
• M Glenet, Y N'Guyen, A Mirand, C Henquell, AL Lebreil, F Berri, F Bani-Sadr, B Lina, I Schuffenec, L Andreolett Major 5'terminally deleted enterovirus populations modulate type I IFN response in acute myocarditis patients and in human cultured cardiomyocytes Sci Rep, 2020-07-20;10(1):11947. 2020-07-20 [PMID: 32686697] (Human)
• M Sermershei, AD Kenney, PH Lin, TM McMichael, C Cai, K Gumpper, TMA Adesanya, H Li, X Zhou, KH Park, JS Yount, J Ma MG53 suppresses interferon-&beta and inflammation via regulation of ryanodine receptor-mediated intracellular calcium signaling Nat Commun, 2020-07-17;11(1):3624. 2020-07-17 [PMID: 32681036] (Human)
• DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells MBio, 2019-07-16;10(4):. 2019-07-16 [PMID: 31311882] (Human)
• H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway Elife, 2019-02-08;8(0):. 2019-02-08 [PMID: 30735121] (Human)
• P An, MT Sáenz Robl, AM Duray, PG Cantalupo, JM Pipas Human polyomavirus BKV infection of endothelial cells results in interferon pathway induction and persistence PLoS Pathog., 2019-01-08;15(1):e1007505. 2019-01-08 [PMID: 30620752] (Human)
• L Strzadala, A Fiedorowic, E Wysokinska, E Ziolo, M Grudzie?, M Jelen, K Pluta, B Morak-Mlod, M Zimecki, W Kalas An Anti-Inflammatory Azaphenothiazine Inhibits Interferon ? Expression and CXCL10 Production in KERTr Cells Molecules, 2018-09-24;23(10):. 2018-09-24 [PMID: 30250011] (Human)
• C Petes, V Mintsopoul, RL Finnen, BW Banfield, K Gee The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages J. Biol. Chem., 2018-09-21;0(0):. 2018-09-21 [PMID: 30242126] (Human)
• MC Mladinich, J Schwedes, ER Mackow Zika Virus Persistently Infects and Is Basolaterally Released from Primary Human Brain Microvascular Endothelial Cells MBio, 2017-07-11;8(4):. 2017-07-11 [PMID: 28698279] (Human)

FAQs for IFN-beta (DY814-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.