Human Haptoglobin DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Haptoglobin DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Haptoglobin DuoSet ELISA, 5 Plate

• binding peptide
• BP
• haptoglobin alpha(1S)-beta
• haptoglobin alpha(2FS)-beta
• Haptoglobin
• haptoglobin, alpha polypeptide
• haptoglobin, beta polypeptide
• HP
• HP-1
• hp2-alpha
• HP2ALPHA2
• HPA1S
• MGC111141
• zonulin

Background

Haptoglobin is an acute phase glycoprotein that is an inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and binds with high affinity to circulating Hemoglobin. Association of Haptoglobin with free Hemoglobin protects tissues from Hemoglobin-mediated oxidative damage and facilitates Hemoglobin clearance. In addition, Haptoglobin has anti-inflammatory properties that are independent of Hemoglobin binding. It inhibits prostaglandin synthesis, scavenges nitric oxide, dampens the inflammatory response to endotoxins, and inhibits T cell proliferation and neutrophil respiratory bursts. Deletion of Haptoglobin in mice facilitates the development of autoimmune inflammation.

Publications for Haptoglobin (DY8465-05)(4)

Publications using DY8465-05

• Asal, M;Thon, M;Waaijman, T;Bontkes, HJ;van Vliet, SJ;Mebius, RE;Gibbs, S; Integration of Stromal Cells and Hydrogel Below Epithelium Results in Optimal Barrier Properties of Small Intestine Organoid Models Biomedicines 2024-12-21 [PMID: 39767819] (Human)
• Custers, E;van der Burgh, YGR;Vreeken, D;Schuren, F;van den Broek, TJ;Verschuren, L;de Blaauw, I;Bouwens, M;Kleemann, R;Kiliaan, AJ;Hazebroek, EJ; Gastrointestinal complaints after Roux-en-Y gastric bypass surgery. Impact of microbiota and its metabolites Heliyon 2024-11-15 [PMID: 39559236] (Human)
• KN Lee, KH Park, YM Kim, I Cho, TE Kim Prediction of emergency cerclage outcomes in women with cervical insufficiency: The role of inflammatory, angiogenic, and extracellular matrix-related proteins in amniotic fluid PLoS ONE, 2022-05-10;17(5):e0268291. 2022-05-10 [PMID: 35536791] (Human)
• JH Back, SY Kim, MB Gu, HJ Kim, KN Lee, JE Lee Proteomic analysis of plasma to identify novel biomarkers for intra-amniotic infection and/or inflammation in preterm premature rupture of membranes Research square, 2023-01-01;0(0):. 2023-01-01 [PMID: 30335284] (Human)

FAQs for Haptoglobin (DY8465-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.