FGF-19 Antibodies

HAF017). A specific band was detected for FGF-19 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for FGF-19 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, conditioned media from COLO 205 cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human FGF-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FGF-19 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_thumb" />

969-FG) was serially diluted and captured by Mouse Anti-Human FGF‑19 Monoclonal Antibody (Catalog # MAB9691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 969-FG) was serially diluted and captured by Mouse Anti-Human FGF‑19 Monoclonal Antibody (Catalog # MAB9691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="FGF-19 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human FGF-19 Monoclonal Antibody (Catalog # MAB969) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

969-FG) was serially diluted and captured by Mouse Anti-Human FGF‑19 Monoclonal Antibody (Catalog # MAB9691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 969-FG) was serially diluted and captured by Mouse Anti-Human FGF‑19 Monoclonal Antibody (Catalog # MAB9691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human FGF‑19 (Catalog # 969-FG) was serially diluted and captured by Mouse Anti-Human FGF‑19 Monoclonal Antibody (Catalog # MAB9691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />