Human Fas Ligand/TNFSF6 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Fas Ligand/TNFSF6 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FASLG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Fas Ligand/TNFSF6 DuoSet ELISA, 15 Plate

• apoptosis (APO-1) antigen ligand 1
• Apoptosis antigen ligand
• APT1LG1CD95L
• APTL
• CD178 antigen
• CD178
• CD95L
• CD95-L
• Fas antigen ligand
• Fas ligand (TNF superfamily, member 6)
• Fas Ligand
• FASLCD95 ligand
• FASLG
• TNFSF6
• TNFSF6FasL
• tumor necrosis factor (ligand) superfamily, member 6
• tumor necrosis factor ligand superfamily member 6

Background

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.

Publications for Fas Ligand/TNFSF6 (DY126)(7)

Publications using DY126

• Lengvenyte, A;Cognasse, F;Hamzeh-Cognasse, H;Sénèque, M;Strumila, R;Olié, E;Courtet, P; Baseline circulating biomarkers, their changes, and subsequent suicidal ideation and depression severity at 6 months: A prospective analysis in patients with mood disorders Psychoneuroendocrinology 2024-07-06 [PMID: 39003840] (Human)
• P Bajgain, AG Torres Cha, K Balasubram, L Fleckenste, P Lulla, HE Heslop, J Vera, AM Leen Secreted Fas decoys enhance the antitumor activity of engineered and bystander T cells in Fas ligand-expressing solid tumors Oncogene, 2022-11-02;0(0):. 2022-11-02 [PMID: 36122411] (Human)
• C Ehrnthalle, S Braumüller, S Kellermann, F Gebhard, M Perl, M Huber-Lang Complement Factor C5a Inhibits Apoptosis of Neutrophils-A Mechanism in Polytrauma? Journal of Clinical Medicine, 2021-07-17;10(14):. 2021-07-17 [PMID: 34300323] (Human)
• JN Tsoporis, E Hatziagela, S Gupta, S Izhar, V Salpeas, A Tsiavou, AG Rigopoulos, AS Triantafyl, JC Marshall, TG Parker, IK Rizos Circulating Ligands of the Receptor for Advanced Glycation End Products and the Soluble Form of the Receptor Modulate Cardiovascular Cell Apoptosis in Diabetes Molecules, 2020-11-10;25(22):. 2020-11-10 [PMID: 33182705] (Human)
• M Kameda, M Otsuka, H Chiba, K Kuronuma, T Hasegawa, H Takahashi, H Takahashi CXCL9, CXCL10, and CXCL11; biomarkers of pulmonary inflammation associated with autoimmunity in patients with collagen vascular diseases-associated interstitial lung disease and interstitial pneumonia with autoimmune features PLoS ONE, 2020-11-02;15(11):e0241719. 2020-11-02 [PMID: 33137121] (Human)
• Hsu HY, Wittemann S, Schneider EM, Weiss M, Joos TO Suspension microarrays for the identification of the response patterns in hyperinflammatory diseases. Med Eng Phys, 2008-03-03;30(8):976-83. 2008-03-03 [PMID: 18313970] (Human)
• Venet F, Pachot A, Debard AL, Bohe J, Bienvenu J, Lepape A, Powell WS, Monneret G Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism. J. Immunol., 2006-11-01;177(9):6540-7. 2006-11-01 [PMID: 17056586] (Human)

FAQs for Fas Ligand/TNFSF6 (DY126). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FASLG