Human Enolase 2/Neuron-specific Enolase DuoSet ELISA, 5 Plt

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Enolase 2/Neuron-specific Enolase DuoSet ELISA, 5 Plt Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ENO2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Enolase 2/Neuron-specific Enolase DuoSet ELISA, 5 Plt

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma-enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.

Publications for Enolase 2/Neuron-specific Enolase (DY5169-05)(4)

Publications using DY5169-05

• Andreou, D;Steen, NE;Mørch-Johnsen, L;Jørgensen, KN;Wortinger, LA;Barth, C;Szabo, A;O'Connell, KS;Lekva, T;Hjell, G;Johansen, IT;Ormerod, MBEG;Haukvik, UK;Aukrust, P;Djurovic, S;Yolken, RH;Andreassen, OA;Ueland, T;Agartz, I; Toxoplasma gondii infection associated with inflammasome activation and neuronal injury Scientific reports 2024-03-04 [PMID: 38438515] (Human)
• M Adeel, CC Chen, BS Lin, HC Chen, JC Liou, YT Li, CW Peng Safety of Special Waveform of Transcranial Electrical Stimulation (TES): In Vivo Assessment International Journal of Molecular Sciences, 2022-06-20;23(12):. 2022-06-20 [PMID: 35743291] (Rat)
• BES de Araújo, R da Silva F, MC de Magalhã, F Lima-Setta, VB Paravidino, PM Riveiro, LB Pulcheri, M Dos Santos, MB Genuíno-Ol, JR Robaina, AJLA da Cunha, FF Cruz, PRM Rocco, FA Bozza, HC de Castro-, A Prata-Barb Clinical features, electroencephalogram, and biomarkers in pediatric sepsis-associated encephalopathy Oncogene, 2022-06-23;12(1):10673. 2022-06-23 [PMID: 35739230] (Human)
• CR Schindler, T Lustenberg, M Woschek, P Störmann, D Henrich, P Radermache, I Marzi Severe Traumatic Brain Injury (TBI) Modulates the Kinetic Profile of the Inflammatory Response of Markers for Neuronal Damage J Clin Med, 2020-06-01;9(6):. 2020-06-01 [PMID: 32492963] (Human)

FAQs for Enolase 2/Neuron-specific Enolase (DY5169-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ENO2