Human Endothelin-1 QuantiGlo ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Endothelin-1 QuantiGlo ELISA Kit Summary

• Background: The QuantiGlo Human Endothelin-1 Chemiluminescent Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Endothelin-1 levels in cell culture supernates, serum, EDTA plasma, and urine without extraction. It contains synthetic human Endothelin-1 and antibodies raised against the synthetic factor. This immunoassay has been shown to accurately quantitate human Endothelin-1. Result...s obtained using natural human Endothelin-1 showed dose curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Endothelin-1.
  Show More
• Specificity: Natural and synthetic human Endothelin-1
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: EDN1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 degreesC. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Endothelin-1 QuantiGlo ELISA Kit

• ARCND3
• EDN1
• endothelin 1
• Endothelin-1
• ET1
• HDLCQ7
• PPET1
• preproendothelin-1
• QME

Background

Endothelin-1 (ET-1), a peptide of 21 amino acid (aa) residues, is a pleiotropic molecule best known for its action as a potent vasoconstrictor (1). Originally isolated from porcine aortic endothelial cells, ET-1 is one of a family of three proteins encoded by distinct genes that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3) (2, 3). ET-2 and ET-3 differ from ET-1 by 2 and 6 amino acids, respectively (1, 2). All members of the Endothelin family contain two essential disulfide bridges and six conserved aa residues at the C-terminus. Human ET-1 is initially synthesized as a pre-pro-polypeptide of 212 amino acids (2, 4). It is proteolytically cleaved by a signal peptidase to produce pro-ET-1 and further processed by a Furin-like protease to yield a 38 aa peptide termed Big ET-1 (5, 6). Big ET-1 is then cleaved by the membrane-bound metalloprotease Endothelin-converting enzyme (ECE-1), producing the potent 21 aa mature form ET-1 (aa 1-21) (7, 8). Alternatively, ET-1 may exist in an active 31 aa form (ET-1 (aa 1-31)) following cleavage of Big ET-1 by chymase (9-12). The vascular endothelium is an abundant source of ET-1 (3, 13). It may also be expressed by leukocytes, smooth muscle cells, mesangial cells, cardiac myocytes, and astrocytes (14, 15). ET-1 can be induced in endothelial cells by many factors including mechanical stimulation, various hormones, and pro-inflammatory cytokines (16). Production is inhibited by nitric oxide (NO), Prostacyclin, and atrial natriuretic peptide (ANP) (17-19). Two receptors for the Endothelin family have been cloned and designated ETA and ETB (20-23). ETA and ETB belong to the large family of heptahelical G protein-coupled receptors. The ETA receptor shows a higher affinity for ET-1 than for ET-2 and lowest affinity for ET-3, while the ETB receptor shows approximately equal affinity for each of the three Endothelins (21, 22, 24). ETA is primarily responsible for the vasoconstrictor effects of ET-1 and is expressed by blood vessel smooth muscle cells (25, 26). The ETB receptor is also present in smooth muscle and the endothelia of blood vessels, kidney, lung, and brain (27). ET-1 has the ability to activate an array of signaling cascades including classical phosphatidylinositol turnover pathways leading to downstream PKC activation and Ca 2+ mobilization (28-32). Other potential signaling mediators activated or produced by ET-1 include PI 3-kinase/Akt, NO, FAK, and Rho GTPases (32-37). ET-1 signaling may also be mediated indirectly via transactivation of the EGF receptor leading to downstream signaling by Ras and MAP kinases (38, 39). Injection of a single dose of ET-1 produces an initial decrease in systemic blood pressure followed by a prolonged increase in blood pressure (16, 40, 41). Blockade of Endothelin receptors with a systemic injection of an ETA/ETB antagonist causes progressive vasodilation, and elevated levels of ET-1 are found in some forms of human hypertension (42, 43). ET-1 also stimulates cardiac contraction and the growth of cardiac myocytes, regulates the release of vasoactive substances, and stimulates smooth muscle cell mitogenesis (32, 44-46). It also acts as a pro-survival factor for endothelial cells and regulates secretion by hypothalamic and pituitary cells (47, 48). ET-1 may control inflammatory responses by promoting the adhesion and migration of neutrophils and stimulating the production of pro-inflammatory cytokines (49-53). It has also been implicated in cancer progression at several levels including regulating the proliferation and migration of tumor cells and acting as a pro-angiogenic factor (54, 55). In addition, ET-1 has putative roles in other pathologies including septic shock, atherosclerosis, heart failure, renal insufficiency, pulmonary hypertension, and cerebrovascular conditions associated with subarachnoid hemorrhage (15, 56-63).

Publications for Endothelin-1 (QET00B)(48)

Publications using QET00B

• Vassiliou, AG;Roumpaki, A;Keskinidou, C;Athanasiou, N;Tsipilis, S;Jahaj, E;Vrettou, CS;Giannopoulou, V;Halioti, A;Ferentinos, G;Dimopoulou, I;Kotanidou, A;Langleben, D;Orfanos, SE; Transpulmonary Plasma Endothelin-1 Arterial:Venous Ratio Differentiates Survivors from Non-Survivors in Critically Ill Patients with COVID-19-Induced Acute Respiratory Distress Syndrome International journal of molecular sciences 2024-10-02 [PMID: 39408968] (Human)
• Gouaref, I;Otmane, A;Makrelouf, M;Abderrhmane, SA;Haddam, AEM;Koceir, EA; Crucial Interactions between Altered Plasma Trace Elements and Fatty Acids Unbalance Ratio to Management of Systemic Arterial Hypertension in Diabetic Patients: Focus on Endothelial Dysfunction International journal of molecular sciences 2024-08-27 [PMID: 39273236] (Human)
• Gonsalves, AM;Baker, SE;Jacob, DW;Harper, JL;Manrique-Acevedo, CM;Limberg, JK; Effect of endothelin-1 on the blood pressure response to acute hypoxia and hyperoxia in healthy young men Physiological reports 2024-09-01 [PMID: 39218615] (Human)
• Cherpaz, M;Meugnier, E;Seillier, G;Pozzi, M;Pierrard, R;Leboube, S;Farhat, F;Vola, M;Obadia, JF;Amaz, C;Chalabreysse, L;May, C;Chanon, S;Brun, C;Givre, L;Bidaux, G;Mewton, N;Derumeaux, G;Bergerot, C;Paillard, M;Thibault, H; Myocardial transcriptomic analysis of diabetic patients with aortic stenosis: key role for mitochondrial calcium signaling Cardiovascular diabetology 2024-07-08 [PMID: 38978010] (Human)
• Fato, BR;Beard, S;Binder, NK;Pritchard, N;Kaitu'u-Lino, TJ;de Alwis, N;Hannan, NJ; The Regulation of Endothelin-1 in Pregnancies Complicated by Gestational Diabetes: Uncovering the Vascular Effects of Insulin Biomedicines 2023-09-28 [PMID: 37893034] (Human)
• Jeong, S;Linder, BA;Barnett, AM;Tharpe, MA;Hutchison, ZJ;Culver, MN;Sanchez, SO;Nichols, OI;Grosicki, GJ;Bunsawat, K;Nasci, VL;Gohar, EY;Fuller-Rowell, TE;Robinson, AT; Interplay of Race and Neighborhood Deprivation on Ambulatory Blood Pressure in Young Adults medRxiv : the preprint server for health sciences 2023-09-12 [PMID: 37745604] (Human)
• LH Willems, LMC Jacobs, LA Groh, H Ten Cate, HMH Spronk, B Wilson-Sto, G Hannink, SMJ van Kuijk, C Ghossein-D, M Nagy, DHJ Thijssen, AS van Peters, MC Warlé Vascular Function, Systemic Inflammation, and Coagulation Activation 18 Months after COVID-19 Infection: An Observational Cohort Study Journal of Clinical Medicine, 2023-02-10;12(4):. 2023-02-10 [PMID: 36835948] (Human)
• L van Doorn, WJ Visser, DCH van Dorst, KM Mirabito C, SLW Koolen, AVE de Mik, IM Garrelds, DM Bovée, EO de Hoop, S Bins, FALM Eskens, EJ Hoorn, AH Jan Danser, RHJ Mathijssen, J Versmissen Dietary sodium restriction prevents vascular endothelial growth factor inhibitor-induced hypertension British Journal of Cancer, 2022-11-10;0(0):1-9. 2022-11-10 [PMID: 36357702] (Human)
• M Haffke, H Freitag, G Rudolf, M Seifert, W Doehner, N Scherbakov, L Hanitsch, K Wittke, S Bauer, F Konietschk, F Paul, J Bellmann-S, C Kedor, C Scheibenbo, F Sotzny Endothelial dysfunction and altered endothelial biomarkers in patients with post-COVID-19 syndrome and chronic fatigue syndrome (ME/CFS) Journal of Translational Medicine, 2022-03-22;20(1):138. 2022-03-22 [PMID: 35317812] (Human)
• AD Kaze, X Gao, SK Musani, A Bidulescu, AG Bertoni, M Abdalla, JB Echouffo-T Association of Plasma Endothelin-1 with Blood Pressure Progression Among African Americans: The Jackson Heart Study American heart journal, 2022-01-02;0(0):. 2022-01-02 [PMID: 34986393] (Human)
• N Guo, P Wang, J Yang, X Yang, M van der Vo, M Wildwater, J Wei, X Tang, M Wang, H Yang Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals Biomarkers Relevant to Personalized Treatments Frontiers in Pharmacology, 2021-06-14;12(0):664320. 2021-06-14 [PMID: 34194326] (Human)
• M Žarak, A Perovi?, M Njire Brat, S Šupraha Go, J Dumi? Adaptive response triggered by the repeated SCUBA diving is reflected in cardiovascular, muscular, and immune biomarkers Physiological Reports, 2021-01-01;9(2):e14691. 2021-01-01 [PMID: 33463896] (Human)
• LG Douma, K Solocinski, SH Masten, DH Barral, SJ Barilovits, LA Jeffers, KD Alder, R Patel, CS Wingo, KD Brown, BD Cain, ML Gumz EDN1-AS, A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells Front Physiol, 2020-03-13;11(0):209. 2020-03-13 [PMID: 32231591] (Human)
• X Yang, W Yang, DG McVey, G Zhao, J Hu, RN Poston, M Ren, K Willeit, S Coassin, J Willeit, TR Webb, NJ Samani, M Mayr, S Kiechl, S Ye FURIN Expression in Vascular Endothelial Cells Is Modulated by a Coronary Artery Disease-Associated Genetic Variant and Influences Monocyte Transendothelial Migration J Am Heart Assoc, 2020-02-11;9(4):e014333. 2020-02-11 [PMID: 32067586] (Human)
• Y Chen, D Wu, L Sun Clinical Significance of High-Mobility Group Box 1 Protein (HMGB1) and Nod-Like Receptor Protein 3 (NLRP3) in Patients with Ulcerative Colitis Med. Sci. Monit., 2020-01-05;26(0):e919530. 2020-01-05 [PMID: 31901930] (Human)
• F Mayyas, N Saadeh, K Al-Muqbel, DR Van Wagone Plasma endothelin-1 levels are increased in atrial fibrillation patients with hyperthyroidism PLoS ONE, 2018-12-04;13(12):e0208206. 2018-12-04 [PMID: 30513109] (Human)
• M Chen, Z Li, Z Zhang, Y Du, Y Zhang, M Xu, C Sun Intervention of integrative medicine treatment has impact on serum levels of ET-1, TNF-?, MLT in RA-CVD Saudi J Biol Sci, 2018-05-14;25(5):959-964. 2018-05-14 [PMID: 30108447] (Human)
• KL Clines, GA Clines DKK1 and Kremen Expression Predicts the Osteoblastic Response to Bone Metastasis Transl Oncol, 2018-05-15;11(4):873-882. 2018-05-15 [PMID: 29772510] (Human)
• BM Fox, BK Becker, AS Loria, KA Hyndman, C Jin, H Clark, R Johns, M Yanagisawa, DM Pollock, JS Pollock Acute Pressor Response to Psychosocial Stress Is Dependent on Endothelium-Derived Endothelin-1 J Am Heart Assoc, 2018-02-16;7(4):. 2018-02-16 [PMID: 29453306] (Mouse)
• M Lantz, K Kostulas, M Settergren, C Sjöstrand Impaired endothelial function in patients with cryptogenic stroke and patent foramen ovale is not affected by closure J Interv Cardiol, 2017-04-25;0(0):. 2017-04-25 [PMID: 28439982] (Human)
• C De Miguel, WC Hamrick, JL Hobbs, DM Pollock, PK Carmines, JS Pollock Endothelin receptor-specific control of endoplasmic reticulum stress and apoptosis in the kidney Sci Rep, 2017-02-23;7(0):43152. 2017-02-23 [PMID: 28230089] (Rat)
• Free radical scavenging decreases endothelin-1 excretion and glomerular albumin permeability during type 1 diabetes Physiol Rep, 2016-12-01;4(24):. 2016-12-01 [PMID: 28039404] (Rat)
• Jun-Ichi Kira Allergic Inflammation Leads to Neuropathic Pain via Glial Cell Activation J. Neurosci., 2016-11-23;36(47):11929-11945. 2016-11-23 [PMID: 27881779] (Human)
• Malcolm Kohler Effect of OSA on hypoxic and inflammatory markers during CPAP withdrawal: Further evidence from three randomized control trials Respirology, 2016-11-18;0(0):. 2016-11-18 [PMID: 27860068] (Human)
• Albuminuria Is Associated with Endothelial Dysfunction and Elevated Plasma Endothelin-1 in Sickle Cell Anemia PLoS ONE, 2016-09-26;11(9):e0162652. 2016-09-26 [PMID: 27669006] (Human)
• Bradley Miller p66Shc regulates renal vascular tone in hypertension-induced nephropathy J Clin Invest, 2016-06-06;0(0):. 2016-06-06 [PMID: 27270176] (Rat)
• Parikh R, Ma Y, Scherzer R, Heringer A, Macgregor J, Martin J, Deeks S, Ganz P, Hsue P Endothelin-1 Predicts Hemodynamically Assessed Pulmonary Arterial Hypertension in HIV Infection. PLoS ONE, 2016-01-11;11(1):e0146355. 2016-01-11 [PMID: 26752193] (Human)
• Yu A, Tam B, Yau W, Chan K, Yu S, Chung T, Siu P Association of endothelin-1 and matrix metallopeptidase-9 with metabolic syndrome in middle-aged and older adults. Diabetol Metab Syndr, 2015-12-10;7(0):111. 2015-12-10 [PMID: 26692905] (Human)
• Roksnoer L, van Veghel R, de Vries R, Garrelds I, Bhaggoe U, Friesema E, Leijten F, Poglitsch M, Domenig O, Clahsen-van Groningen M, Hoorn E, Jan Danser A, Batenburg W Optimum AT1 receptor-neprilysin inhibition has superior cardioprotective effects compared with AT1 receptor blockade alone in hypertensive rats. Kidney Int, 2015-04-01;88(1):109-20. 2015-04-01 [PMID: 25830765] (Rat)
• Ranchoux B, Gunther S, Quarck R, Chaumais M, Dorfmuller P, Antigny F, Dumas S, Raymond N, Lau E, Savale L, Jais X, Sitbon O, Simonneau G, Stenmark K, Cohen-Kaminsky S, Humbert M, Montani D, Perros F Chemotherapy-induced pulmonary hypertension: role of alkylating agents. Am J Pathol, 2014-12-11;185(2):356-71. 2014-12-11 [PMID: 25497573] (Rabbit)
• Tannheimer S, Sorensen E, Cui Z, Kim M, Patel L, Baker W, Phillips G, Wright C, Salmon M The in vitro pharmacology of GS-5759, a novel bifunctional phosphodiesterase 4 inhibitor and long acting beta2-adrenoceptor agonist. J Pharmacol Exp Ther, 2014-02-10;349(1):85-93. 2014-02-10 [PMID: 24513870] (Human)
• Wojciak-Stothard B, Abdul-Salam V, Lao K, Tsang H, Irwin D, Lisk C, Loomis Z, Stenmark K, Edwards J, Yuspa S, Howard L, Edwards R, Rhodes C, Gibbs J, Wharton J, Zhao L, Wilkins M Aberrant chloride intracellular channel 4 expression contributes to endothelial dysfunction in pulmonary arterial hypertension. Circulation, 2014-02-06;129(17):1770-80. 2014-02-06 [PMID: 24503951] (Human)
• Guo F, Carter D, Leask A miR-218 regulates focal adhesion kinase-dependent TGFbeta signaling in fibroblasts. Mol Biol Cell, 2014-02-05;25(7):1151-8. 2014-02-05 [PMID: 24501422] (Human)
• Guo F, Carter DE, Mukhopadhyay A, Leask A Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair? PLoS ONE, 2011-11-02;6(11):e27097. 2011-11-02 [PMID: 22073262] (Human)
• Botden IP, Langendonk JG, Meima ME Daily red wine consumption improves vascular function by a soluble guanylyl cyclase-dependent pathway. Am. J. Hypertens., 2010-11-18;24(2):162-8. 2010-11-18 [PMID: 21088673] (Human)
• Pham I, Wuerzner G, Richalet JP, Peyrard S, Azizi M Endothelin receptors blockade blunts hypoxia-induced increase in PAP in humans. Eur. J. Clin. Invest., 2010-03-01;40(3):195-202. 2010-03-01 [PMID: 20415698] (Human)
• Loria AS, D'Angelo G, Pollock DM, Pollock JS Early life stress downregulates endothelin receptor expression and enhances acute stress-mediated blood pressure responses in adult rats. Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010-04-21;299(1):R185-91. 2010-04-21 [PMID: 20410476] (Rat)
• Lund AK, Lucero J, Lucas S, Madden MC, McDonald JD, Seagrave JC, Knuckles TL, Campen MJ Vehicular emissions induce vascular MMP-9 expression and activity associated with endothelin-1-mediated pathways. Arterioscler. Thromb. Vasc. Biol., 2009-01-15;29(4):511-7. 2009-01-15 [PMID: 19150882] (Human)
• Casanova G, Radavelli S, Lhullier F, Spritzer PM Effects of nonoral estradiol-micronized progesterone or low-dose oral estradiol-drospirenone therapy on metabolic variables and markers of endothelial function in early postmenopause. Fertil. Steril., 2008-08-15;92(2):605-12. 2008-08-15 [PMID: 18706557] (Human)
• Torgrimson BN, Meendering JR, Miller NP, Kaplan PF, Minson CT Endothelial function, endothelin-1, and fibrinogen in young women using the vaginal contraceptive ring. Fertil. Steril., 2008-08-15;92(2):441-7. 2008-08-15 [PMID: 18706555] (Human)
• Carroll VA, Ashcroft M Regulation of angiogenic factors by HDM2 in renal cell carcinoma. Cancer Res., 2008-01-15;68(2):545-52. 2008-01-15 [PMID: 18199551] (Human)
• Pegorier S, Wagner LA, Gleich GJ, Pretolani M Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells. J. Immunol., 2006-10-01;177(7):4861-9. 2006-10-01 [PMID: 16982928] (Human)
• Menzies-Gow NJ, Bailey SR, Stevens K, Katz L, Elliott J, Marr CM Digital blood flow and plasma endothelin concentration in clinically endotoxemic horses. Am. J. Vet. Res., 2005-04-01;66(4):630-6. 2005-04-01 [PMID: 15900943] (Equine)
• von Lueder TG, Kjekshus H, Edvardsen T, ØIe E, Urheim S, Vinge LE, Ahmed MS, Smiseth OA, Attramadal H Mechanisms of elevated plasma endothelin-1 in CHF: congestion increases pulmonary synthesis and secretion of endothelin-1. Cardiovasc. Res., 2004-07-01;63(1):41-50. 2004-07-01 [PMID: 15194460] (Porcine)
• Kuklin VN, Kirov MY, Evgenov OV, Sovershaev MA, Sjoberg J, Kirova SS, Bjertnaes LJ Novel endothelin receptor antagonist attenuates endotoxin-induced lung injury in sheep. Crit. Care Med., 2004-03-01;32(3):766-73. 2004-03-01 [PMID: 15090960] (Ovine)
• Sasser JM, Pollock JS, Pollock DM Renal endothelin in chronic angiotensin II hypertension. Am. J. Physiol. Regul. Integr. Comp. Physiol., 2002-07-01;283(1):R243-8. 2002-07-01 [PMID: 12069950] (Rat)
• Watt G, Kantipong P, Jongsakul K, de Souza M, Burnouf T Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study. QJM, 2001-11-01;94(11):599-607. 2001-11-01 [PMID: 11704689] (Human)
• Reinhart GA, 2018, Preusser LC, Opgenorth TJ, Wegner CD, Cox BF Endothelin and ET(A) receptors in long-term arterial pressure homeostasis in conscious nonhuman primates. 1364165, 2000-11-01;279(5):R1701-6. 2000-11-01 [PMID: 11049852] (Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))

Bioinformatics

• Gene Symbol: EDN1