Human Dkk-1 Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human Dkk-1 Quantikine ELISA Kit Summary

Background
The Quantikine® Human Dkk-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure human Dkk-1 in cell culture supernates, serum-free cell culture supernates, serum, and platelet-poor plasma. It contains Sf 21-expressed recombinant human Dkk-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Dkk-1 showed dose-respon...se curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Dkk-1.
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Specificity
Natural and recombinant human Dkk-1.
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DKK100B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Dkk-1 Quantikine ELISA Kit

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Dkk-1 (DKK100B)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.


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Human
(11)
Mouse
(1)
Rat
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using DKK100B Applications Species
M Diani, S Perego, V Sansoni, L Bertino, M Gomarasca, M Faraldi, PDM Pigatto, G Damiani, G Banfi, G Altomare, G Lombardi Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients Int J Mol Sci, 2019;20(22):. 2019 [PMID: 31717649] (Human) Human
S Mihai, E Codrici, ID Popescu, AM Enciu, E Rusu, D Zilisteanu, LG Necula, G Anton, C Tanase Inflammation-Related Patterns in the Clinical Staging and Severity Assessment of Chronic Kidney Disease Dis. Markers, 2019;2019(0):1814304. 2019 [PMID: 31687048] (Human) Human
C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms J. Cell. Mol. Med., 2018;0(0):. 2018 [PMID: 29975001] (Human) Human
DR Jadon, R Sengupta, A Nightingal, H Lu, J Dunphy, A Green, JT Elder, RP Nair, E Korendowyc, MA Lindsay, NJ McHugh Serum bone-turnover biomarkers are associated with the occurrence of peripheral and axial arthritis in psoriatic disease: a prospective cross-sectional comparative study Arthritis Res. Ther., 2017;19(1):210. 2017 [PMID: 28934972] (Human) Human
X Tang, J Lin, G Wang, J Lu MicroRNA-433-3p promotes osteoblast differentiation through targeting DKK1 expression PLoS ONE, 2017;12(6):e0179860. 2017 [PMID: 28628652] (Rat) Rat
TH Kim, JS Chang, KS Park, J Park, N Kim, JI Lee, ID Kong Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial PLoS ONE, 2017;12(2):e0171771. 2017 [PMID: 28178355] (Human) Human
CC Niu, SS Lin, LJ Yuan, LH Chen, CY Yang, AN Chung, ML Lu, TT Tsai, PL Lai, WJ Chen Correlation of blood bone turnover biomarkers and Wnt signaling antagonists with AS, DISH, OPLL, and OYL BMC Musculoskelet Disord, 2017;18(1):61. 2017 [PMID: 28153008] (Human) Human
EJ Lee, SM Kim, B Choi, EY Kim, YH Chung, EJ Lee, B Yoo, CK Lee, S Hong, BJ Kim, JM Koh, SH Kim, YG Kim, EJ Chang Interleukin-32 Gamma Stimulates Bone Formation by Increasing miR-29a in Osteoblastic Cells and Prevents the Development of Osteoporosis Sci Rep, 2017;7(0):40240. 2017 [PMID: 28079119] (Human) Human
Hartman M, Talar B, Noman M, Gajos-Michniewicz A, Chouaib S, Czyz M Gene expression profiling identifies microphthalmia-associated transcription factor (MITF) and Dickkopf-1 (DKK1) as regulators of microenvironment-driven alterations in melanoma phenotype. PLoS ONE, 2014;9(4):e95157. 2014 [PMID: 24733089] (Human) Human
Andre T, Meuleman N, Stamatopoulos B, De Bruyn C, Pieters K, Bron D, Lagneaux L Evidences of early senescence in multiple myeloma bone marrow mesenchymal stromal cells. PLoS ONE, 2013;8(3):e59756. 2013 [PMID: 23555770] (Human) Human
Show All 14 Publications.

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Product General Protocols

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FAQs for Dkk-1 (DKK100B). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DKK100B

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Diseases for Dkk-1 (DKK100B)

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Blogs on Dkk-1.

The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

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