Human Cystatin C Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Cystatin C Quantikine ELISA Kit Summary

• Background: The Quantikine Human Cystatin C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cystatin C in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Cystatin C and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cystatin C showed linear curves that were paral...lel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Cystatin C.
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• Specificity: Natural and recombinant human Cystatin C
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CST3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Cystatin C Quantikine ELISA Kit

• ARMD11
• bA218C14.4 (cystatin C)
• CST3
• cystatin 3
• cystatin C (amyloid angiopathy and cerebral hemorrhage)
• Cystatin C
• cystatin-3
• cystatin-C
• Gamma-trace
• MGC117328
• Neuroendocrine basic polypeptide
• Post-gamma-globulin

Background

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Cystatin C (DSCTC0)(32)

Publications using DSCTC0

• Lin, GH;Lin, CH;Wang, JS; Associations of cystatin C with incident chronic kidney disease and all-cause mortality in patients with normal glucose tolerance and prediabetes Scientific reports 2025-07-02 [PMID: 40596202] (Human)
• Divyaveer, S;Kashyap, M;Kekan, K;Yadav, AK;Kaur, J;Premkumar, M;Gandotra, A;Prajapati, K;De, A;Duseja, AK;Verma, N;Aggarwal, R;Venkatasubramanian, V;Tiwari, V;Bagur, V;Patil, AN;Safiq, N;Kohli, HS; Comparison of Measured Glomerular Filtration Rate Versus Estimated Glomerular Filtration Rate in Indian Cirrhotic Patients: Report of a Pilot Study Journal of clinical and experimental hepatology 2024-11-22 [PMID: 39760116] (Human)
• Hoskin, PJ;Malhi, A;Reczko, K;Hackshaw, A; Urinary biomarkers in metastatic bone pain: Results from a multicentre randomized trial of ibandronate compared to single dose radiotherapy for localized metastatic bone pain in prostate cancer (RIB) Journal of bone oncology 2024-08-01 [PMID: 39148581] (Human)
• Musia?, K;Stojanowski, J;Mi?kiewicz-Bujna, J;Ka?wak, K;Ussowicz, M; KIM-1, IL-18, and NGAL, in the Machine Learning Prediction of Kidney Injury among Children Undergoing Hematopoietic Stem Cell Transplantation-A Pilot Study International journal of molecular sciences 2023-10-31 [PMID: 37958774] (Human)
• Downes, KJ;Zuppa, AF;Sharova, A;Neely, MN; Optimizing Vancomycin Therapy in Critically Ill Children: A Population Pharmacokinetics Study to Inform Vancomycin Area under the Curve Estimation Using Novel Biomarkers Pharmaceutics 2023-04-25 [PMID: 37242578] (Human)
• MD Politis, M Yao, C Gennings, M Tamayo-Ort, D Valvi, S Kim-Schulz, J Qi, C Amarasiriw, I Pantic, MC Tolentino, G Estrada-Gu, JH Greenberg, MM Téllez-Roj, RO Wright, AP Sanders, MJ Rosa Prenatal Metal Exposures and Associations with Kidney Injury Biomarkers in Children Toxics, 2022-11-16;10(11):. 2022-11-16 [PMID: 36422900] (Human)
• CP Pérez-Mace, E Flores-Alf, LDC Alarcón-Ro, A Vences-Vel, N Castro-Ala, E Martínez-M, M Ramirez CD14 and CD26 from serum exosomes are associated with type 2 diabetes, exosomal Cystatin C and CD14 are associated with metabolic syndrome and atherogenic index of plasma PeerJ, 2022-07-12;10(0):e13656. 2022-07-12 [PMID: 35846887] (Human)
• AP Sanders, C Gennings, M Tamayo-Ort, S Mistry, I Pantic, M Martinez, G Estrada-Gu, A Espejel-Nu, LT Olascoaga, RO Wright, MM Téllez-Roj, M Arora, C Austin Prenatal and early childhood critical windows for the association of nephrotoxic metal and metalloid mixtures with kidney function Oncogene, 2022-06-27;166(0):107361. 2022-06-27 [PMID: 35797845] (Human)
• A Batte, S Menon, J Ssenkusu, S Kiguli, R Kalyesubul, J Lubega, EI Mutebi, RO Opoka, CC John, MC Starr, AL Conroy Acute kidney injury in hospitalized children with sickle cell anemia Bmc Nephrology, 2022-03-18;23(1):110. 2022-03-18 [PMID: 35303803] (Human)
• A Longobardi, R Nicsanu, S Bellini, R Squitti, M Catania, P Tiraboschi, C Saraceno, C Ferrari, R Zanardini, G Binetti, G Di Fede, L Benussi, R Ghidoni Cerebrospinal Fluid EV Concentration and Size Are Altered in Alzheimer's Disease and Dementia with Lewy Bodies Cells, 2022-01-28;11(3):. 2022-01-28 [PMID: 35159272] (Human)
• H Wada, T Shinozaki, M Suzuki, S Sakagami, Y Ajiro, J Funada, M Matsuda, M Shimizu, T Takenaka, Y Morita, K Yonezawa, H Matsubara, Y Ono, T Nakamura, K Fujimoto, A Ninomiya, T Kato, T Unoki, D Takagi, K Wada, M Wada, M Iguchi, H Yamakage, T Kusakabe, A Yasoda, A Shimatsu, K Kotani, N Satoh-Asah, M Abe, M Akao, K Hasegawa, EXCEED?J S Impact of Chronic Kidney Disease on the Associations of Cardiovascular Biomarkers With Adverse Outcomes in Patients With Suspected or Known Coronary Artery Disease: The EXCEED-J Study Journal of the American Heart Association, 2022-01-20;11(3):e023464. 2022-01-20 [PMID: 35048713] (Human)
• JM Forbes, S Le Bagge, S Righi, AK Fotheringh, LA Gallo, DA McCarthy, S Leung, T Baskervill, J Nisbett, A Morton, S Teasdale, N D'Silva, H Barrett, T Jones, J Couper, K Donaghue, N Isbel, DW Johnson, L Donnellan, P Deo, LK Akison, KM Moritz, T O'Moore-Su Advanced glycation end products as predictors of renal function in youth with type 1 diabetes Scientific Reports, 2021-05-03;11(1):9422. 2021-05-03 [PMID: 33941808] (Human)
• C Saylor, M Tamayo-Ort, I Pantic, C Amarasiriw, N McRae, G Estrada-Gu, S Parra-Hern, MC Tolentino, AA Baccarelli, JJ Fadrowski, C Gennings, LM Satlin, RO Wright, MM Tellez-Roj, AP Sanders Prenatal blood lead levels and reduced preadolescent glomerular filtration rate: Modification by body mass index Environment international, 2021-03-04;0(0):106414. 2021-03-04 [PMID: 33678412] (Human)
• C Kosmeri, H Milionis, AP Vlahos, T Benekos, E Bairaktari, V Cholevas, E Siomou The impact of dyslipidemia on early markers of endothelial and renal dysfunction in children Journal of clinical lipidology, 2020-12-11;0(0):. 2020-12-11 [PMID: 33478934] (Human)
• W Wo?yniec, K Kasprowicz, J Giebu?towi, N Korytowska, K Zorena, M Bartoszewi, P Rita-Tkach, M Renke, W Ratkowski Changes in Water Soluble Uremic Toxins and Urinary Acute Kidney Injury Biomarkers After 10- and 100-km Runs Int J Environ Res Public Health, 2019-10-28;16(21):. 2019-10-28 [PMID: 31661892] (Human)
• D Kostic, GPNS Beozzo, SB do Couto, AHT Kato, L Lima, P Palmeira, VLJ Krebs, V Bunduki, RPV Francisco, M Zugaib, FT Dénes, WB de Carvalh, VHK Koch The role of renal biomarkers to predict the need of surgery in congenital urinary tract obstruction in infants J Pediatr Urol, 2019-03-16;0(0):. 2019-03-16 [PMID: 30979613] (Human)
• LS Nguyen, V Spagnoli, M Kerneis, M Hauguel-Mo, O Barthélémy, JP Collet, G Montalesco, J Silvain Evaluation of neutrophil gelatinase-associated lipocalin and cystatin C as biomarkers of acute kidney injury after ST-segment elevation myocardial infarction treated by percutaneous coronary intervention Arch Cardiovasc Dis, 2019-01-09;0(0):. 2019-01-09 [PMID: 30639197] (Human)
• MC Edman, SR Janga, Z Meng, M Bechtold, AF Chen, C Kim, L Naman, A Sarma, N Teekappana, AY Kim, S Madrigal, S Singh, E Ortiz, S Christiana, DG Arkfeld, WJ Mack, M Heur, W Stohl, SF Hamm-Alvar Increased Cathepsin S activity associated with decreased protease inhibitory capacity contributes to altered tear proteins in Sj�gren's Syndrome patients Sci Rep, 2018-07-23;8(1):11044. 2018-07-23 [PMID: 30038391] (Human)
• I Lousa, H Nascimento, S Rocha, C Catarino, F Reis, C Rêgo, A Santos-Sil, A Seabra, S Ribeiro, L Belo Influence of the 6-month physical activity programs on renal function in obese boys Pediatr. Res., 2018-04-11;0(0):. 2018-04-11 [PMID: 29638229] (Human)
• R Scherzer, SJ Shah, E Secemsky, J Butler, C Grunfeld, MG Shlipak, PY Hsue Association of Biomarker Clusters With Cardiac Phenotypes and Mortality in Patients With HIV Infection Circ Heart Fail, 2018-04-01;11(4):e004312. 2018-04-01 [PMID: 29615435] (Human)
• V Drossou-Ag Neonatal acute kidney injury following Valsartan exposure in utero: report of two cases Hippokratia, 2016-01-01;20(1):73-75. 2016-01-01 [PMID: 27895448] (Human)
• Identifying biomarkers of dementia prevalent among amnestic mild cognitively impaired ethnic female patients Alzheimers Res Ther, 2016-10-18;8(1):43. 2016-10-18 [PMID: 27756387] (Human)
• Ali H, Hussain N, Naim M, Zayed M, Al-Mulla F, Kehinde E, Seaburg L, Sundsbak J, Harris P A novel PKD1 variant demonstrates a disease-modifying role in trans with a truncating PKD1 mutation in patients with autosomal dominant polycystic kidney disease. BMC Nephrol, 2015-03-01;16(0):26. 2015-03-01 [PMID: 25880449] (Human)
• Tung Y, Chang C, Chen Y, Chu P Combined Biomarker Analysis for Risk of Acute Kidney Injury in Patients with ST-Segment Elevation Myocardial Infarction. PLoS ONE, 2015-04-08;10(4):e0125282. 2015-04-08 [PMID: 25853556] (Human)
• Kay , Paul, Yang , Yit C, Hiscott , Paul, Gray , Donna, Maminishkis , Arvydas, Paraoan , Luminita Age-related changes of cystatin C expression and polarized secretion by retinal pigment epithelium: potential age-related macular degeneration links. Invest Ophthalmol Vis Sci, 2014-02-14;55(2):926-34. 2014-02-14 [PMID: 24458156] (Human)
• Chen TH, Chang CH, Lin CY, Jenq CC, Chang MY, Tian YC, Hung CC, Fang JT, Yang CW, Wen MS, Lin FC, Chen YC Acute kidney injury biomarkers for patients in a coronary care unit: a prospective cohort study. PLoS ONE, 2012-02-22;7(2):e32328. 2012-02-22 [PMID: 22384218] (Human)
• Kim PS, Woods C, Dutcher L, Georgoff P, Rosenberg A, Mican JA, Kopp JB, Smith MA, Hadigan C Increased prevalence of albuminuria in HIV-infected adults with diabetes. PLoS ONE, 2011-09-13;6(9):e24610. 2011-09-13 [PMID: 21931772] (Human)
• Gunawardana CG, Kuk C, Smith CR Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer. J. Proteome Res., 2009-10-01;8(10):4705-13. 2009-10-01 [PMID: 19663500] (Human)
• Tsuji-Akimoto S, Yabe I, Niino M, Kikuchi S, Sasaki H Cystatin C in cerebrospinal fluid as a biomarker of ALS. Neurosci. Lett., 2009-03-06;452(1):52-5. 2009-03-06 [PMID: 19444952] (Human)
• Chudleigh RA, Ollerton RL, Dunseath G Use of cystatin C-based estimations of glomerular filtration rate in patients with type 2 diabetes. Diabetologia, 2009-05-09;52(7):1274-8. 2009-05-09 [PMID: 19430759] (Human)
• Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE A urinary biomarker profile for children with HIV-associated renal diseases. Kidney Int., 2009-04-08;76(2):207-14. 2009-04-08 [PMID: 19357719] (Human)
• Tsai HT, Wang PH, Tee YT, Lin LY, Hsieh YS, Yang SF Imbalanced serum concentration between cathepsin B and cystatin C in patients with pelvic inflammatory disease. Fertil. Steril., 2008-03-07;91(2):549-55. 2008-03-07 [PMID: 18314113] (Human)

FAQs for Cystatin C (DSCTC0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CST3