Human CXCL16 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CXCL16 Quantikine ELISA Kit Summary

• Background: The Quantikine Human CXCL16 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CXCL16 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CXCL16 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CXCL16 showed linear curves that were parallel to the standard curves obt...ained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CXCL16.
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• Specificity: Natural and recombinant human CXCL16
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CXCL16

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL16 Quantikine ELISA Kit

• chemokine (C-X-C motif) ligand 16
• CXC chemokine ligand 16
• CXCL16
• CXCLG16
• Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
• SCYB16
• Small-inducible cytokine B16
• SRPSOXC-X-C motif chemokine 16
• SR-PSOXTransmembrane chemokine CXCL16

Background

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein that has a non-ELR CXC chemokine domain in its extracellular region. Fractalkine (CX3CL1) and CXCL16 constitute the only two transmembrane chemokines. Mouse and human CXCL16 share 70% amino acid sequence similarity within their chemokine domains and 49% overall amino acid sequence identity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL16 (DCX160)(14)

Publications using DCX160

• T Guan, F Emscherman, C Schories, P Groga-Bada, P Martus, O Borst, M Gawaz, T Geisler, D Rath, M Chatterjee Platelet SR-PSOX/CXCL16-CXCR6 Axis Influences Thrombotic Propensity and Prognosis in Coronary Artery Disease International Journal of Molecular Sciences, 2022-09-21;23(19):. 2022-09-21 [PMID: 36232370] (Human)
• C Helissey, N Guitard, H Théry, S Goulinet, P Mauduit, M Girleanu, AL Favier, M Drouet, C Parnot, C Chargari, S Cavallero, S François Two New Potential Therapeutic Approaches in Radiation Cystitis Derived from Mesenchymal Stem Cells: Extracellular Vesicles and Conditioned Medium Biology, 2022-06-28;11(7):. 2022-06-28 [PMID: 36101361] (Human)
• K Prajzlerov, O Kryšt?fkov, P Hánová, V Horváthová, M Gregová, K Pavelka, J Vencovský, L Šenolt, M Filková High miR-451 expression in peripheral blood mononuclear cells from subjects at risk of developing rheumatoid arthritis Scientific Reports, 2021-02-25;11(1):4719. 2021-02-25 [PMID: 33633196] (Human)
• Z Ma, C Ma, Q Zhang, Y Bai, K Mu, X Liu, Q Yang Role of CXCL16 in BLM-induced epithelial-mesenchymal transition in human A549 cells Respiratory Research, 2021-02-06;22(1):42. 2021-02-06 [PMID: 33549109] (Human)
• FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk Systemic Inflammation Precedes Microalbuminuria in Diabetes Kidney Int Rep, 2019-06-21;4(10):1373-1386. 2019-06-21 [PMID: 31701047] (Human)
• RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival Oncotarget, 2017-07-07;8(37):62081-62098. 2017-07-07 [PMID: 28977928] (Human)
• Gosuke Takiguchi, Michiru Nishita, Kana Kurita, Yoshihiro Kakeji, Yasuhiro Minami Wnt5a-Ror2 signaling in mesenchymal stem cells promotes proliferation of gastric cancer cells by activating CXCL16-CXCR6 axis. Cancer Science, 2016-02-09;0(0):1349-7006. 2016-02-09 [PMID: 26708384] (Human)
• Zeng M, Yan H, Chen Y, Zhao H, Lv Y, Liu C, Zhou P, Zhao B Suppression of NF-kappaB Reduces Myocardial No-Reflow. PLoS ONE, 2012-10-09;7(10):e47306. 2012-10-09 [PMID: 23056624] (Human)
• Santhekadur PK, Das SK, Gredler R, Chen D, Srivastava J, Robertson C, Baldwin AS, Fisher PB, Sarkar D Multifunction protein staphylococcal nuclease domain containing 1 (SND1) promotes tumor angiogenesis in human hepatocellular carcinoma through novel pathway that involves nuclear factor κB and miR-221. J. Biol. Chem., 2012-03-06;287(17):13952-8. 2012-03-06 [PMID: 22396537] (Human)
• Huang M, Han Y, Zhang X An intron polymorphism in the CXCL16 gene is associated with increased risk of coronary artery disease in Chinese Han population: a large angiography-based study. Atherosclerosis, 2009-11-10;210(1):160-5. 2009-11-10 [PMID: 19954776] (Human)
• Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis. Cancer Res., 2008-12-01;68(23):9996-10003. 2008-12-01 [PMID: 19047182] (Human)
• Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma. Int. J. Oncol., 2008-08-01;33(2):297-308. 2008-08-01 [PMID: 18636150] (Human)
• Smith C, Halvorsen B, Otterdal K, Waehre T, Yndestad A, Fevang B, Sandberg WJ, Breland UM, Froland SS, Oie E, Gullestad L, Damas JK, Aukrust P High levels and inflammatory effects of soluble CXC ligand 16 (CXCL16) in coronary artery disease: down-regulatory effects of statins. Cardiovasc. Res., 2008-03-13;79(1):195-203. 2008-03-13 [PMID: 18339644] (Human)
• Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas. Glia, 2008-05-01;56(7):764-74. 2008-05-01 [PMID: 18293410] (Human)

FAQs for CXCL16 (DCX160). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CXCL16