Human Complement Factor D Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Complement Factor D Quantikine ELISA Kit Summary

• Background: The Quantikine Human Complement Factor D Immunoassay is a 3.5 hour sandwich ELISA designed to measure human Complement Factor D in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human Complement Factor D and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Complement Factor D showed linear curves th...at were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Complement Factor D.
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• Specificity: Natural and recombinant human Complement Factor D
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CFD

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Complement Factor D Quantikine ELISA Kit

• Adipsin
• ADN
• ADNcomplement factor D
• AMBP-1
• C3 convertase activator
• CFD
• complement factor D (adipsin)
• complement factor D preproprotein
• Complement Factor D
• D component of complement (adipsin)
• DF
• EC 3.4.21
• EC 3.4.21.46
• PFD
• Properdin factor DADIPSIN

Background

Complement Factor D, also known as adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3.3 Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Complement Factor D/Adipsin (DFD00)(6)

Publications using DFD00

• Dumitrescu, G;Antovic, J;Soutari, N;Gran, C;Antovic, A;Al-Abani, K;Grip, J;Rooyackers, O;Taxiarchis, A; The role of complement and extracellular vesicles in the development of pulmonary embolism in severe COVID-19 cases PloS one 2024-08-23 [PMID: 39178205] (Human)
• Yusuke Murakami, Mitsuhiro Nishigori, Hiroaki Yagi, Tsukasa Osaki, Masaki Wakabayashi, Manabu Shirai et al. Serum proteomic identification and validation of two novel atherosclerotic aortic aneurysm biomarkers, profilin 1 and complement factor D Proteome Science 2023-08-05 [PMID: 37543598] (Human)
• M Milek, Y Moulla, M Kern, C Stroh, A Dietrich, MR Schön, D Gärtner, T Lohmann, M Dressler, P Kovacs, M Stumvoll, M Blüher, E Guiu-Jurad Adipsin Serum Concentrations and Adipose Tissue Expression in People with Obesity and Type 2 Diabetes International Journal of Molecular Sciences, 2022-02-17;23(4):. 2022-02-17 [PMID: 35216336] (Human)
• AM Risitano, AG Kulasekara, JW Lee, JP Maciejewsk, R Notaro, R Brodsky, M Huang, M Geffner, P Browett Danicopan: an oral complement factor D inhibitor for paroxysmal nocturnal hemoglobinuria Haematologica, 2021-12-01;0(0):0. 2021-12-01 [PMID: 33121236] (Human)
• Small-molecule Factor D inhibitors selectively block the alternative pathway of complement in paroxysmal nocturnal hemoglobinuria and atypical hemolytic uremic syndrome Haematologica, 2016-11-03;0(0):. 2016-11-03 [PMID: 27810992] (Human)
• Reynolds R, Hartnett ME, Atkinson JP, Giclas PC, Rosner B, Seddon JM Plasma complement components and activation fragments: associations with age-related macular degeneration genotypes and phenotypes. Invest. Ophthalmol. Vis. Sci., 2009-08-06;50(12):5818-27. 2009-08-06 [PMID: 19661236] (Human)

FAQs for Complement Factor D/Adipsin (DFD00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CFD