Human CCL4/MIP-1 beta DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CCL4/MIP-1 beta DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL4/MIP-1 beta DuoSet ELISA, 15 Plate

• ACT2
• ACT-2
• AT744.1
• C-C motif chemokine 4
• CCL4
• chemokine (C-C motif) ligand 4
• Exodus-3
• G-26 T-lymphocyte-secreted protein
• G-26
• HC21
• LAG1
• LAG-1
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• MIP1 beta
• MIP-1 beta
• MIP1B
• MIP1B1
• MIP-1-beta
• MIP-1-beta(1-69)
• PAT 744
• Protein H400
• SCYA2
• SCYA4
• secreted protein G-26
• SIS-gamma
• small inducible cytokine A4 (homologous to mouse Mip-1b)
• Small-inducible cytokine A4
• T-cell activation protein 2

Background

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.

Publications for CCL4/MIP-1 beta (DY271)(32)

Publications using DY271

• Haynes, AM;Cohen, SB;Pesaladinne, A;Sherman, DR;Urdahl, KB;Cox, JS;Hawn, TR; ESX-5 Deletions in Mycobacterium tuberculosis Alter Macrophage Cytokine Signaling and Bacterial Heavy Metal Response bioRxiv : the preprint server for biology 2025-10-02 [PMID: 41256704] (Human)
• Gkiokas, A;Papadatou-Gigante, M;Gkioka, AI;Koudouna, A;Tryfou, TM;Alexandropoulos, A;Bartzi, V;Kafasi, N;Kyrtsonis, MC; Tumor-Associated Macrophage (TAM)-Related Cytokines, sCD163, CCL2, and CCL4, as Novel Biomarkers for Overall Survival and Time to Treatment in Waldenstrom's Macroglobulinemia: Emphasis on Asymptomatic WM Cells 2025-02-13 [PMID: 39996747] (Human)
• Chai, N;Stachon, T;Häcker, S;Berger, T;Li, Z;Amini, M;Suiwal, S;Seitz, B;Langenbucher, A;Szentmáry, N; The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways PloS one 2025-01-27 [PMID: 39869570] (Human)
• Bekirian, C;Valsecchi, I;Bachellier-Bassi, S;Scandola, C;Guijarro, JI;Chauvel, M;Mourer, T;Gow, NAR;Aimanianda, VK;d'Enfert, C;Fontaine, T; ?-1,6-Glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wall eLife 2024-12-05 [PMID: 39636210] (Human)
• Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M; Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls PloS one 2024-07-15 [PMID: 39008499] (Human)
• deKay, JT;Chepurko, E;Chepurko, V;Knudsen, L;Lord, C;Searight, M;Tsibulnikov, S;Robich, MP;Sawyer, DB;Gagnon, DJ;May, T;Riker, R;Seder, DB;Ryzhov, S; Delayed CCL23 response is associated with poor outcomes after cardiac arrest Cytokine 2024-02-06 [PMID: 38325139]
• Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D; Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial PloS one 2023-11-02 [PMID: 37917760] (Human)
• Jeon, S;Jeon, JH;Jeong, J;Kim, G;Lee, S;Kim, S;Maruthupandy, M;Lee, K;Yang, SI;Cho, WS; Size- and oxidative potential-dependent toxicity of environmentally relevant expanded polystyrene styrofoam microplastics to macrophages Journal of hazardous materials 2023-08-14 [PMID: 37597397] (Human)
• MJ Tosiek, K Groesser, A Pekcec, M Zwirek, G Murugesan, E Borges Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response Journal of Immunology Research, 2022-02-25;2022(0):9926305. 2022-02-25 [PMID: 35252461] (Human)
• S Jamieson, A Mawdesley, D Deehan, J Kirby, J Holland, A Tyson-Capp Inflammatory responses to metal oxide ceramic nanopowders Scientific Reports, 2021-05-18;11(1):10531. 2021-05-18 [PMID: 34006936] (Human)
• L Wang, D Aschenbren, Z Zeng, X Cao, D Mayr, M Mehta, M Capitani, N Warner, J Pan, L Wang, Q Li, T Zuo, S Cohen-Keda, J Lu, RC Ardy, DJ Mulder, D Dissanayak, K Peng, Z Huang, X Li, Y Wang, X Wang, S Li, S Bullers, AN Gammage, K Warnatz, AI Schiefer, G Krivan, V Goda, WHA Kahr, M Lemaire, Genomics E, CY Lu, I Siddiqui, MG Surette, D Kotlarz, KR Engelhardt, HR Griffin, R Rottapel, H Decaluwe, RM Laxer, M Proietti, S Hambleton, S Elcombe, CH Guo, B Grimbacher, I Dotan, SC Ng, SA Freeman, SB Snapper, C Klein, K Boztug, Y Huang, D Li, HH Uhlig, AM Muise Gain-of-function variants in SYK cause immune dysregulation and systemic inflammation in humans and mice Nature Genetics, 2021-03-29;53(4):500-510. 2021-03-29 [PMID: 33782605] (Human)
• GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation Biomolecules, 2021-02-21;11(2):. 2021-02-21 [PMID: 33669910] (Human)
• H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist Frontiers in Pharmacology, 2020-12-08;11(0):598702. 2020-12-08 [PMID: 33363471] (Human)
• E Yeregui, C Viladés, P Domingo, A Ceausu, YM Pacheco, S Veloso, A Inciarte, J Vidal-Gonz, M Peraire, C Perpiñán, V Falcó, J Masip, V Alba, M Vargas, A Martí, L Reverté, J Mallolas, F Vidal, J Peraire, A Rull High circulating SDF-1and MCP-1 levels and genetic variations in CXCL12, CCL2 and CCR5: Prognostic signature of immune recovery status in treated HIV-positive patients EBioMedicine, 2020-11-06;62(0):103077. 2020-11-06 [PMID: 33166788] (Human)
• G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5 Cell Death Dis, 2019-03-19;10(4):264. 2019-03-19 [PMID: 30890699] (Human)
• Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL J. Cell. Mol. Med., 2018-10-24;0(0):. 2018-10-24 [PMID: 30353642] (Human)
• New Insights into the Pro-Inflammatory Activities of Ang1 on Neutrophils: Induction of MIP-1? Synthesis and Release PLoS ONE, 2016-09-15;11(9):e0163140. 2016-09-15 [PMID: 27632174] (Human)
• McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women. PLoS ONE, 2015-08-06;10(8):e0134619. 2015-08-06 [PMID: 26247200] (Human)
• Cohen-Kedar S, Baram L, Elad H, Brazowski E, Guzner-Gur H, Dotan I Human intestinal epithelial cells respond to beta-glucans via Dectin-1 and Syk. Eur J Immunol, 2014-10-27;44(12):3729-40. 2014-10-27 [PMID: 25251945] (Human)
• Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand. Nat Commun, 2014-06-09;5(0):4092. 2014-06-09 [PMID: 24910129] (Human)
• Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro. PLoS ONE, 2014-05-12;9(5):e96925. 2014-05-12 [PMID: 24819779] (Human)
• Yamaguchi Y, Shao Z, Sharif S, Du X, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung L Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage. J Biol Chem, 2012-11-30;288(5):3097-111. 2012-11-30 [PMID: 23204518] (Human)
• Dumitru CA, Fechner MK, Hoffmann TK A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer. J. Leukoc. Biol., 2012-01-18;91(4):591-8. 2012-01-18 [PMID: 22262799] (Human)
• Pedrosa E, Carretero-Iglesia L, Boada A, Colobran R, Faner R, Pujol-Autonell I, Palou E, Esteve A, Pujol-Borrell R, Ferrandiz C, Juan M, Carrascosa JM CCL4L Polymorphisms and CCL4/CCL4L Serum Levels Are Associated with Psoriasis Severity. J. Invest. Dermatol., 2011-05-26;131(9):1830-7. 2011-05-26 [PMID: 21614014] (Human)
• Savage ND, De Boer T, Walburg KV, Joosten SA, van Meijgaarden K, Geluk A, Ottenhoff TH Human anti-inflammatory macrophages induce Foxp3+ GITR+ CD25+ regulatory T cells, which suppress via membrane-bound TGFbeta-1. J. Immunol., 2008-08-01;181(3):2220-6. 2008-08-01 [PMID: 18641362] (Human)
• Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation. Am. J. Transplant., 2008-07-01;8(7):1512-22. 2008-07-01 [PMID: 18513272] (Human)
• Lamhamedi-Cherradi SE, Martin RE, Ito T, Kheradmand F, Corry DB, Liu YJ, Moyle M Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12. J. Immunol., 2008-05-01;180(9):6000-9. 2008-05-01 [PMID: 18424720] (Human)
• Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J. Clin. Invest., 2007-12-01;117(12):3664-3672. 2007-12-01 [PMID: 17992260] (Human)
• Brito A, Almeida A, Gonsalez CR, Mendonca M, Ferreira F, Fernandes SS, Duarte AJ, Casseb J Successful HAART is associated with high B-chemokine levels in chronic HIV type 1-infected patients. AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):906-12. 2007-07-01 [PMID: 17678475] (Human)
• Walker J, Tough DF Modification of TLR-induced activation of human dendritic cells by type I IFN: synergistic interaction with TLR4 but not TLR3 agonists. Eur. J. Immunol., 2006-07-01;36(7):1827-36. 2006-07-01 [PMID: 16783851] (Human)
• Holl V, Hemmerter S, Burrer R, Schmidt S, Bohbot A, Aubertin AM, Moog C Involvement of Fc gamma RI (CD64) in the mechanism of HIV-1 inhibition by polyclonal IgG purified from infected patients in cultured monocyte-derived macrophages. J. Immunol., 2004-11-15;173(10):6274-83. 2004-11-15 [PMID: 15528366] (Human)
• Choi EM, Chen JL, Wooldridge L, Salio M, Lissina A, Lissin N, Hermans IF, Silk JD, Mirza F, Palmowski MJ, Dunbar PR, Jakobsen BK, Sewell AK, Cerundolo V High avidity antigen-specific CTL identified by CD8-independent tetramer staining. J. Immunol., 2003-11-15;171(10):5116-23. 2003-11-15 [PMID: 14607910] (Human)

FAQs for CCL4/MIP-1 beta (DY271). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL4