Human CCL27/CTACK DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CCL27/CTACK DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL27

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL27/CTACK DuoSet ELISA, 15 Plate

• ALP
• C-C motif chemokine 27
• CCL27
• chemokine (C-C motif) ligand 27
• CTACK
• CTACKSmall-inducible cytokine A27
• CTAK
• cutaneous T-cell attracting chemokine
• Cutaneous T-cell-attracting chemokine
• ESKINE
• IL-11 Ralpha-locus chemokine
• ILC
• ILCCC chemokine ILC
• PESKY
• SCYA27IL-11 R-alpha-locus chemokine
• skinkine
• small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also known as Cutaneous T cell-attracting chemokine (CTACK), ALP, ILC and ESkine, is a CC chemokine discovered using expressed sequence tags (EST) in a homology search for novel chemokines. Mature human and mouse CCL27 are 84% similar. Mouse CCL27 shares 37% and 35% amino acid identity with mouse MIP-3 beta and mouse TECK, respectively. CCL27 is predominantly expressed in the skin. The expression is up-regulated by proinflammatory cytokines.

Publications for CCL27/CTACK (DY376)(6)

Publications using DY376

• W Francuzik, K Pažur, M Dalke, S Dölle-Bier, M Babina, M Worm Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis JCI Insight, 2022-04-08;0(0):. 2022-04-08 [PMID: 35202004] (Human)
• Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 2017-09-01;97(0):133-140. 2017-09-01 [PMID: 28648867] (Human)
• Kraynyak KA, Kutzler MA, Cisper NJ, Khan AS, Draghia-Akli R, Sardesal NY, Lewis MG, Yan J, Weiner DB Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques. Vaccine, 2010-02-23;28(8):1942-51. 2010-02-23 [PMID: 20188250] (Human)
• Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene. J. Immunol., 2009-02-01;182(3):1270-7. 2009-02-01 [PMID: 19155472] (Human)
• Baumgartner-Nielsen J, Vestergaard C, Thestrup-Pedersen K Glucocorticoid-induced tumour necrosis factor receptor (GITR) and its ligand (GITRL) in atopic dermatitis. Acta Derm. Venereol., 2006-01-01;86(5):393-8. 2006-01-01 [PMID: 16955181] (Human)
• Vestergaard C, Johansen C, Otkjaer K, Deleuran M, Iversen L Tumor necrosis factor-alpha-induced CTACK/CCL27 (cutaneous T-cell-attracting chemokine) production in keratinocytes is controlled by nuclear factor kappaB. Cytokine, 2005-01-21;29(2):49-55. 2005-01-21 [PMID: 15598438] (Human)

FAQs for CCL27/CTACK (DY376). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL27