Human Aggrecan DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Aggrecan DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ACAN

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Aggrecan DuoSet ELISA, 15 Plate

• ACAN
• AGC1
• AGC1SEDK
• aggrecan core protein
• Aggrecan
• Cartilage-specific proteoglycan core protein
• Chondroitin sulfate proteoglycan 1
• Chondroitin sulfate proteoglycan core protein 1
• CSPCP
• CSPG1
• CSPG1aggrecan 1
• CSPGCP
• large aggregating proteoglycan
• MSK16
• MSK16AGCAN
• SEDK

Background

Aggrecan belongs to the chondroitin sulfate (CS) proteoglycan family, which also includes Versican, Brevican, and Neurocan. Each Aggrecan molecule contains approximately 100 and 30 keratan sulfate and glycosaminoglycan (GAG) sidechains, respectively. Aggrecan non-covalently associates with hyaluronan via the link modules and an Ig domain in its N-terminus. It is the most abundant proteoglycan in cartilage, and contributes to the load-bearing capacity of this tissue.

Publications for Aggrecan (DY1220)(12)

Publications using DY1220

• Snuggs, JW;Senter, RK;Whitt, JP;Jackson, JD;Le Maitre, CL; PCRX-201, a novel IL-1Ra gene therapy treatment approach for low back pain resulting from intervertebral disc degeneration Gene therapy 2024-11-21 [PMID: 39572769] (Human)
• Sun, R;Zhu, J;Sun, K;Gao, L;Zheng, B;Shi, J; Strontium Ranelate Ameliorates Intervertebral Disc Degeneration via Regulating TGF-?1/NF-?B Axis International journal of medical sciences 2023-10-07 [PMID: 37928874] (Human)
• L Jain, SM Bolam, AP Monk, JT Munro, E Chen, J Tamatea, N Dalbeth, RC Poulsen Differential Effects of Hypoxia versus Hyperoxia or Physoxia on Phenotype and Energy Metabolism in Human Chondrocytes from Osteoarthritic Compared to Macroscopically Normal Cartilage International Journal of Molecular Sciences, 2023-04-19;24(8):. 2023-04-19 [PMID: 37108698] (Human)
• X Wang, D Wang, B Deng, L Yan Syringaresinol attenuates osteoarthritis via regulating the NF-kappaB pathway International immunopharmacology, 2023-03-28;118(0):109982. 2023-03-28 [PMID: 36989902] (Mouse)
• Z Zhou, L Zhang, Y Liu, C Huang, W Xia, H Zhou, Z Zhou, X Zhou Luteolin Protects Chondrocytes from H2O2-Induced Oxidative Injury and Attenuates Osteoarthritis Progression by Activating AMPK-Nrf2 Signaling Oxidative Medicine and Cellular Longevity, 2022-02-01;2022(0):5635797. 2022-02-01 [PMID: 35154568] (Mouse)
• J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants Bone Rep, 2020-09-11;13(0):100715. 2020-09-11 [PMID: 32995386] (Human)
• XH Xue, JX Xue, W Hu, FL Shi, Y Yang Nomilin targets the Keap1-Nrf2 signalling and ameliorates the development of osteoarthritis J. Cell. Mol. Med., 2020-06-21;0(0):. 2020-06-21 [PMID: 32564468] (Mouse)
• D Chen, D Lu, H Liu, E Xue, Y Zhang, P Shang, X Pan Pharmacological blockade of PCAF ameliorates osteoarthritis development via dual inhibition of TNF-alpha-driven inflammation and ER stress EBioMedicine, 2019-11-14;0(0):. 2019-11-14 [PMID: 31735552] (Human)
• Z Li, K Zhang, X Li, H Pan, S Li, F Chen, J Zhang, Z Zheng, J Wang, H Liu Wnt5a suppresses inflammation-driven intervertebral disc degeneration via a TNF-α/NF-κB -Wnt5a negative-feedback loop Osteoarthr. Cartil., 2018-04-12;0(0):. 2018-04-12 [PMID: 29656141] (Rat)
• F Wang, L Han, X Wang, Y Li, Y Zhu, J Wang, C Xue Sialoglycoprotein isolated from eggs of Carassius auratus promotes fracture healing in osteoporotic mice J Food Drug Anal, 2017-09-22;26(2):716-724. 2017-09-22 [PMID: 29567242] (Mouse)
• A Beierfu beta , H Dietrich, C Kremser, M Hunjadi, A Ritsch, T Rülicke, C Thomé, DS Mern Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders PLoS ONE, 2017-11-03;12(11):e0187564. 2017-11-03 [PMID: 29099857] (Rabbit)
• MK Kaneva, KV Greco, SE Headland, T Montero-Me, P Mori, K Greenslade, C Pitzalis, A Moore, M Perretti Identification of Novel Chondroprotective Mediators in Resolving Inflammatory Exudates J. Immunol, 2017-02-27;0(0):. 2017-02-27 [PMID: 28242648] (Human)

FAQs for Aggrecan (DY1220). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ACAN